ENO1Polyclonal ANTIBODYCatalog Number:11204-1-AP Featured Product 24 Publications

For Research Use Only

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Basic Information Catalog Number:11204-1-APSize:40 μg/150 μlSource:RabbitIsotype:IgGPurif ication Method:Antigen aff inity purif icationImmunogen Catalog Number:AG1692

GenBank Accession Number:BC015641GeneID (NCBI):2023Full Name:enolase 1, (alpha)Calculated MW:47 kDaObserved MW:47 kDa

Recommended Dilutions:WB 1:500-1:1000 IP 0.5-4.0 ug for IP and 1:500-1:1000 for WBIHC 1:100-1:400 IF 1:50-1:500

Applications Tested Applications:FC, IF, IHC, IP, WB, ELISACited Applications:IF, IHC, IP, WBSpecies Specif icity:human,mouse,ratCited Species:human, mouse, ratNote-IHC: suggested an gen retrieval withTE buffer pH 9.0; (*) Alterna vely, an genretrieval may be performed with citratebuffer pH 6.0

Positive Controls:WB : human skeletal muscle tissue; PC-3 cells,SGC-7901 cells, mouse skeletal muscle tissueIP : mouse skeletal muscle tissue;IHC : human colon cancer tissue; human livercancer tissue, human stomach cancer tissueIF : HepG2 cells;

Background Information Enolase is an important glycolytic enzyme involved in the interconversion of 2-phosphoglycerate to phosphoenolpyruvate.Enolase were down-regulated inoxLDL-treated cells or in VLDL-treated cells .And it identif ied which are associated with glucose metabolism were down-regulated in the process of foam cellsformation.(19756395)

Notable Publications Author Pubmed ID Journal Application

Bing Sun 27684953 PLoS One WB

Si Gao 28910549 Can J Physiol Pharmacol WB

Xixi Guo 31480692 Biomolecules WB

Storage Storage:Store at -20ºC. Stable for one year af ter shipment.Storage Buffer:PBS with 0.1% sodium azide and 50% glycerol pH 7.3.Aliquoting is unnecessary for -20ºC storage

T: 1 (888) 4PTGLAB (1-888-478-4522) (tollf ree in USA), or 1(312) 455-8498 (outsideUSA)

E: proteintech@ptglab.com W: ptglab.com

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This product is exclusively available underProteintech Group brand and is not available topurchase from any other manufacturer.

Selected Validation Data

human skeletal muscle tissue were subjected toSDS PAGE followed by western blot with 11204-1-AP(ENO1 antibody) at dilution of 1:500incubated at room temperature for 1.5 hours

IP Result of anti-ENO1 (IP:11204-1-AP, 3ug;Detection:11204-1-AP 1:500) with mouse skeletalmuscle tissue lysate 3500ug.

Immunohistochemical analysis of paraff in-embedded human colon cancer tissue slide using11204-1-AP( ENO1 Antibody) at dilution of 1:200(under 10x lens). heat mediated antigen retrievedwith Tris-EDTA buffer(pH9).

Immunohistochemical analysis of paraff in-embedded human colon cancer tissue slide using11204-1-AP( ENO1 Antibody) at dilution of 1:200(under 40x lens). heat mediated antigen retrievedwith Tris-EDTA buffer(pH9).

Immunofluorescent analysis of ( -20 Ethanol )f ixed HepG2 cells using 11204-1-AP(ENO1antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Aff iniPure Goat Anti-Rabbit IgG(H+L)

1X10̂ 6 HeLa cells were stained with 0.2ug ENO1antibody (11204-1-AP, red) and control antibody(blue). Fixed with 90% MeOH blocked with 3%BSA (30 min). Alexa Fluor 488-conjugatedAff iniPure Goat Anti-Rabbit IgG(H+L) with dilution1:1500.