TMEM173/STING PolyclonalantibodyCatalog Number:19851-1-AP Featured Product 42 Publications

For Research Use Only

www.ptglab.com

Basic Information Catalog Number:19851-1-AP

Size:150UL , Concentration: 333 μg/ml byBradford method using BSA as thestandard;

Source:Rabbit

Isotype:IgG

Immunogen Catalog Number:AG13921

GenBank Accession Number:BC047779

GeneID (NCBI):340061

Full Name:transmembrane protein 173

Calculated MW:379 aa, 42 kDa

Observed MW:35-40 kDa, 80 kDa

Purification Method:Antigen affinity purification

Recommended Dilutions:WB 1:1000-1:4000 IP 0.5-4.0 ug for IP and 1:500-1:2000for WBIHC 1:20-1:200 IF 1:50-1:500

Applications Tested Applications:FC, IF, IHC, IP, WB, ELISA

Cited Applications:IF, IHC, IP, WB

Species Specificity:human, mouse, rat

Cited Species:human, mouse, rat

Note-IHC: suggested antigen retrieval withTE buffer pH 9.0; (*) Alternatively, antigenretrieval may be performed with citratebuffer pH 6.0

Positive Controls:

WB : HEK-293 cells, HL-60 cells, HT-29 cells, HepG2cells, human heart tissue, mouse spleen tissue, Rajicells, rat heart tissue, mouse thymus

IP : mouse spleen tissue,

IHC : human spleen tissue, human tonsillitis tissue, ratspleen tissue

IF : HepG2 cells,

Background Information TMEM173, also named as ERIS, MITA, STING and MPYS, is a facilitator of innate immune signaling that promotes theproduction of type I IFN (IFN-alpha and IFN-beta). TMEM173 mediates death signaling via activation of theextracellular signal-regulated kinase (ERK) pathway. There’re monomer (35-40 kDa)(PMID: 27324217;29632140;30918080) and dimer (80 kDa) form of TMEM173 in western blot test.

Notable Publications Author Pubmed ID Journal Application

Lili Tao 32886065 Elife WB,IP

Dianyun Ren 33042621 Am J Cancer Res WB

Yue Guo 33115500 Cell Commun Signal IF

Storage Storage:Store at -20°C. Stable for one year after shipment.Storage Buffer:PBS with 0.02% sodium azide and 50% glycerol pH 7.3.

Aliquoting is unnecessary for -20ºC storage

T: 1 (888) 4PTGLAB (1-888-478-4522) (toll freein USA), or 1(312) 455-8498 (outside USA)

E: proteintech@ptglab.com W: ptglab.com

For technical support and original validation data for this product please contact: This product is exclusively available under ProteintechGroup brand and is not available to purchase from anyother manufacturer.

Selected Validation Data

Various lysis were subjected to SDS PAGE followedby western blot with 19851-1-AP (TMEM173/STINGantibody) at dilution of 1:2000 incubated at roomtemperature for 1.5 hours.

Various lysates were subjected to SDS PAGEfollowed by western blot with 19851-1-AP(TMEM173/STING antibody) at dilution of 1:3000incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded human spleen using 19851-1-AP(TMEM173/STING antibody) at dilution of 1:100(under 10x lens).

Immunohistochemical analysis of paraffin-embedded human spleen using 19851-1-AP(TMEM173/STING antibody) at dilution of 1:100(under 40x lens).

IP Result of anti-TMEM173/STING (IP:19851-1-AP,4ug; Detection:19851-1-AP 1:1000) with mousespleen tissue lysate 4000ug.

Immunofluorescent analysis of (-20 Ethanol) fixedHepG2 cells using 19851-1-AP (TMEM173/STINGantibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

1X10^6 MCF-7 cells were stained with 0.2ugTMEM173/STING antibody (19851-1-AP, red) andcontrol antibody (blue). Fixed with 90% MeOHblocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)with dilution 1:1000.