Monoclonal ANTIBODY Myoglobin
2
Myoglobin Monoclonal antibody Catalog Number: 66205-1-Ig For Research Use Only www.ptglab.com Basic Information Catalog Number: 66205-1-Ig Size: 407 μg/ml Source: Mouse Isotype: IgG1 Immunogen Catalog Number: AG8979 GenBank Accession Number: BC014547 GeneID (NCBI): 4151 Full Name: myoglobin Calculated MW: 154 aa, 17 kDa Observed MW: 17-18 kDa Purification Method: Protein A purification CloneNo.: 1A4F7 Recommended Dilutions: WB 1:5000-1:80000 IHC 1:50-1:500 Applications Tested Applications: IHC, WB, ELISA Species Specificity: human, rat, mouse, rabbit, pig Note-IHC: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 Positive Controls: WB : human skeletal muscle tissue, pig heart tissue, rat heart tissue, mouse heart tissue, rabbit heart tissue, human heart tissue IHC : human heart tissue, Background Information Myoglobin is a cytoplasmic hemoprotein that is expressed primarily in cardiomyocytes and oxidative skeletal muscle fibers, functioning on facilitating oxygen transport and modulating nitric oxide homeostasis within cardiac and skeletal myocytes. Recent studies indicated that myoglobin was also expressed in non-muscle tissues. This antibody well recognized endogenous myoglobin in muscle lysates. Storage Storage: Store at -20°C. Stable for one year after shipment. Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Aliquoting is unnecessary for -20 º C storage T: 4006900926 E: [email protected] W: ptgcn.com For technical support and original validation data for this product please contact: This product is exclusively available under Proteintech Group brand and is not available to purchase from any other manufacturer.
Transcript of Monoclonal ANTIBODY Myoglobin
Myoglobin Monoclonal antibodyCatalog Number:66205-1-Ig
For Research Use Only
www.ptglab.com
Basic Information Catalog Number:66205-1-Ig
Size:407 μg/ml
Source:Mouse
Isotype:IgG1
Immunogen Catalog Number:AG8979
GenBank Accession Number:BC014547
GeneID (NCBI):4151
Full Name:myoglobin
Calculated MW:154 aa, 17 kDa
Observed MW:17-18 kDa
Purification Method:Protein A purification
CloneNo.:1A4F7
Recommended Dilutions:WB 1:5000-1:80000 IHC 1:50-1:500
Applications Tested Applications:IHC, WB, ELISA
Species Specificity:human, rat, mouse, rabbit, pig
Note-IHC: suggested antigen retrieval withTE buffer pH 9.0; (*) Alternatively, antigenretrieval may be performed with citratebuffer pH 6.0
Positive Controls:
WB : human skeletal muscle tissue, pig heart tissue, ratheart tissue, mouse heart tissue, rabbit heart tissue,human heart tissue
IHC : human heart tissue,
Background Information Myoglobin is a cytoplasmic hemoprotein that is expressed primarily in cardiomyocytes and oxidative skeletalmuscle fibers, functioning on facilitating oxygen transport and modulating nitric oxide homeostasis within cardiacand skeletal myocytes. Recent studies indicated that myoglobin was also expressed in non-muscle tissues. Thisantibody well recognized endogenous myoglobin in muscle lysates.
Storage Storage:Store at -20°C. Stable for one year after shipment.Storage Buffer:PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Aliquoting is unnecessary for -20ºC storage
T: 4006900926 E: [email protected] W: ptgcn.comFor technical support and original validation data for this product please contact: This product is exclusively available under Proteintech
Group brand and is not available to purchase from anyother manufacturer.
Selected Validation Data
human skeletal muscle tissue were subjected toSDS PAGE followed by western blot with 66205-1-Ig (Myoglobin antibody at dilution of 1:50000incubated at room temperature for 1.5 hours.
Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66205-1-Ig (Myoglobin antibody at dilution of 1:200 (under10x lens).
Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66205-1-Ig (Myoglobin antibody at dilution of 1:200 (under40x lens).
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