Online Resource 4

S10sh

RNA GFP

Con

trol

shRNA A

tg-7

SS

shRNA A

tg-5

SS

shRNA G

FP C

ontro

l SS

p62

β-Actin43kDa

62kDa

Day

0 1 2 3 4 5 6 7

Via

ble

Cell

Nu

mb

er

0

2.0x105

4.0x105

6.0x105

8.0x105

106

1.2x106

1.4x106

1.6x106ScrambleATG-7 KD 1,25D33

Day

0 1 2 3 4 5 6 7

Via

ble

Cel

l N

um

ber

0

105

2x105

3x105

4x105

5x105

6x105

7x105

ScrambleATG-5 KD 1,25D3

S11 S12

Online Resource 4S10 Autophagic Flux in cells with knockdown of Atg5 or Atg7 Control cells stably expressing shRNA against GFP and cells in which either ATG-5 or ATG 7 was knocked down were serum starved for 72 hours and p62 degradation was measured by western blot analysis. SS (Serum Starvation)S11/S12 Sensitivity to 1,25 D3 in cells with knockdown of Atg5 or Atg7 Cells were exposed to 100nm 1,25D3 and viability was determined based on trypan blue exclusion.

shRNA GFP Control shRNA GFP Control shRNA ATG-7 1,25D3shRNA ATG-5 1,25D3