ISOMET CORPORATION
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BRIEFS E n z y m a t i c D e t e r m i n a t i o n o f P o l y u n s a t u r a t e d Fa t ty A c i d s
A rapid enzymatic method is developed for determining polyunsaturated fatty acids using lipoxidase. The enzyme produces the conjugated diene hydroperoxide which is measured at 234 πΐμ. Direct measurements are made in fats, oils, fatty acids, blood plasma, microorganisms, and plant seed with good sensitivity and accuracy.
JOSEPH MacGEE, The Procter and Gamble Co., Cincinnati, Ohio
Anal. Chem. 31,298 (1959)
Spot Tests fo r A r o m a t i c C o m p o u n d s U s i n g 2 , 4 , 7 -Tr in i t ro f tuorenone
A qualitative spot test for aromatic compounds is devised by spraying spots on filter paper with 2,4,7-trinitro-fluorenone. Color and fluorescence vary with the type of aromatic compound reacting. Certain complexes are extractable in iso-octane and ethyl alcohol.
H. T. GORDON and M. J. HURAUX, University of California, Berkeley, Calif.
Anal. Chem. 31,302 (1959)
A n a l y s i s o f L o n g - C h a i n Fat ty A c i d s b y G a s - L i q u i d C h r o m a t o g r a p h y . M i c r o m e t h o d for P r e p a r a t i o n o f M e t h y l Esters
A technique is devised for the quantitative formation of methyl esters of long-chain fatty acids. The esters, separated in pure form by sublimation, ate ready for gas-liquid chromatographic studies. The technique eliminates saponification with its several disadvantages.
WILHELM STOFFEL, FLORENCE CHU, and E. H. AHRENS, Jr., Rockefeller Institute, New York 2 1 , Ν. Υ.
Anal. Chem. 3 1 , 307 (1959)
D e t e r m i n a t i o n o f Q u i n q u e v a l e n t , T r i v a l e n t , a n d O r g a n i c Phosphorus in the A t m o s p h e r e a n d in A q u e o u s Solut ions
A method for determining quinquevalent, trivalent, and organic phosphorous in mixtures is based on the oxidation of organic phosphorous with alkaline ammonium persulfate. Calculations are made by differences. Application is made to atmospheric and aqueous phosphorous content.
ROY MAY, Tennessee Valley Authority, Wilson Dam, Ala.
Anal. Chem. 3 1 , 308 (1959)
M i c r o d e t e r m i n a t i o n o f A c e t o n e in B io log ica l Fluids
A micromethod for determining acetone is based on the diffusion of the acetone on a filter paper strip into 2,4-dinitrophenylhydrazine solution. The color produced after the addition of sodium hydroxide is read in a photoelectric colorimeter. Interference due to acetaldehyde can be calculated. Method is applicable to biological materials.
M. U. TSAO, G. H. LOWREY, and E. J. GRAHAM, University of Michigan Medical School, Ann Arbor, Mich.
Anal. Chem. 3 1 , 311 (1959)
NaCI KBr CsBr Csl
CELL WINDOWS FOR INFRARED
SPECTROMETERS • All commonly used cell windows stocked for im
mediate shipment. Available either polished and optically f lat or in the form of cleaved or sawed blanks.
• Special cell windows made to order. Let us quote on your requirements.
• ISOMET optical cell windows have excellent transmission characteristics and are precision finished.
• Repair Service available for damaged cell windows and prisms.
• Attractive prices enable you to stretch your laboratory budget.
Write for Technical Bulletin 7 57K
I S O M E T CORPORATION
P. 0 . Box 3 4 6 Palisades Park, N . J . —SPECIALISTS IN CRYSTAL GROW/NG AND FABRICATION—
Circle No. 57 on Readers' Service Card
Locate and Interpret SPECTROGRAMS
FASTER-EASIER WITH
S ADTLER... STANDARD SPECTRA—MIDGET EDITION
• A really serviceable collection of 13,750 usableorganiccompounds • Indexed by ASTM-IBM cards—5 Indices—Specfinder • Identification not complete until compared with known spectra
5 SADTLER INDICES • Alphabetical—Chemical Classes—Empirical Formula • Numerical—Uses, Alphabetical
SPECFINDER • Reduces time for locating, examining and verifying Spectra to
2 or 3 minutes • Reduces need and expense of mechanical sorter • Answers inadequacies of shaker and machine sorter systems
COMMERCIAL SPECTRA • Twenty five groups of usable materials like Surface Active
Agents, Rosins, Plasticizers, Polyols, Soaps, Fatty Oils, Drying Oils, Waxes, Polymers and Pigments and Dyes.
SA0T/I SADTLER RESEARCH LABORATORIES SERVICES 1517 VINE STREET LOcust 4-0835 PHILA. 2, PA.
Circle No. 75 on Readers' Service Card
VOL. 3 1 , NO. 2, FEBRUARY 1959 · 1 7 A