Immunofluorescence Antibody Customer Review for Anti-Phospho-IκB-α (S32/S36) Polyclonal Antibody...
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Result “A signal was detected using Phospho-IkappaB-alpha (S32/S26). Recent reports place IkappaB-alpha (S32/S26) at the axon inial segment (AIS). However, no enrichment at the AIS was detected. ” - P. Brites , Instuto de Biologia Molecular e Celular-IBMC. Cell Line: In vitro cultured primary hip- pocampal neurons Method of validaon: Immunofluorescence Primary Anbody: STJ90311 An-Phospho-IκB-α (S32/S36) Secondary Anbody: Donkey an-rabbit AF568 Diluon rao: 1:250, 1:1000 Protocol Neurons were fixed with 2%PFA Cells were washed 3 mes for 5 minutes with PBS Cells were permeabilised with 0.2% triton X100 for 5 minutes at RT Cells were washed 3 mes for 5 minutes with PBS Blocking occured with 0.5% fish gelan in PBS for 1 hour at 37ºC The primary anbody was incubated overnight at 4ºC and then diluted to 1:250 or 1:1000 in 0.5% fish gelan in PBS Cells were washed 3 mes for 5 minutes with PBS The secondary anbody was incubated at RT for 1 hour and then diluted to 1:1000 in 0.5% fish gelan in PBS Cells were washed 3 mes for 5 minutes with PBS Cells were mounted in fluoroshield with DAPI moulding medium Anbody Customer Review: STJ90311 An-Phospho-IκB-α (S32/S36) Anbody Rang: 1:250 diluon 1:1000 diluon
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Transcript of Immunofluorescence Antibody Customer Review for Anti-Phospho-IκB-α (S32/S36) Polyclonal Antibody...
Result
“A signal was detected using Phospho-IkappaB-alpha (S32/S26). Recent reports place IkappaB-alpha (S32/S26) at the axon initial segment (AIS). However, no enrichment at the AIS was detected.” - P. Brites , Instituto de Biologia Molecular e Celular-IBMC.
Cell Line: In vitro cultured primary hip-pocampal neurons
Method of validation: Immunofluorescence
Primary Antibody: STJ90311 Anti-Phospho-IκB-α (S32/S36)
Secondary Antibody: Donkey anti-rabbit AF568
Dilution ratio: 1:250, 1:1000
Protocol
Neurons were fixed with 2%PFA
Cells were washed 3 times for 5 minutes with PBS
Cells were permeabilised with 0.2% triton X100 for 5 minutes at RT
Cells were washed 3 times for 5 minutes with PBS
Blocking occured with 0.5% fish gelatin in PBS for 1 hour at 37ºC
The primary antibody was incubated overnight at 4ºC and then diluted to 1:250 or 1:1000 in 0.5% fish gelatin in PBS
Cells were washed 3 times for 5 minutes with PBS
The secondary antibody was incubated at RT for 1 hour and then diluted to 1:1000 in 0.5% fish gelatin in PBS
Cells were washed 3 times for 5 minutes with PBS
Cells were mounted in fluoroshield with DAPI moulding medium
Antibody Customer Review: STJ90311 Anti-Phospho-IκB-α (S32/S36) Antibody Rating:
1:250 dilution
1:1000 dilution
