AMPK Signaling Pathway - media.cellsignal.com · Phospho-AMPKα (Thr172) Sandwich ELISA Kit #9839...

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www.cellsignal.com/ampk For pathway key and background please visit: Other RTKs α-Adrenergic Receptor AMPKα β γ PFKFB3 LKB1 MO25α STRAD Adiponectin Receptor Leptin PKA Ras Rheb PLCβ Gα q SirT1 GS TBC1D1 ACC CPT1 Malonyl CoA CaMKK HMG-CoA Reductase FIP200 TSC2 TSC1 PGC1α Insulin SREBP-1 Fatty Acid Synthase HSL ATGL Akt B-Raf Erk p90RSK PI3K Insulin Receptor p70 S6K 4E-BP1 Beclin mTOR GβL Raptor ULK1 PI3K Class III ATG13 Atg14 p300 HNF4α TORC2/ CRTC2 CREB FoxO HDAC7 HDAC5 HDAC4 CRY1 Glut4 vesicle Glut1 vesicle Sterol/Isoprenoid Synthesis Fatty Acid Oxidation Lipolysis Glycolysis Glycogen Synthesis [cAMP] Low Glucose, Hypoxia, Ischemia, Heat Shock AICAR Exercise Brain [Ca 2+ ] Histamine Thrombin Glut Trafficking Glut4 Glut1 Autophagy Lipogenesis AMP + ADP ATP Gluconeogenesis Glucose Glut Translocation Microtubules Actin Dynamics MBS85 MYPT1 TXNIP Myosin Light Chain2 CLIP170 Tau C ytoskeletal Signaling A c u t e R e g u l a t i o n o f M e t a b o li s m Transcriptional C ontrol of M eta b olis m P r o t e in M e ta b o lis m a n d A u t o p h a g y Energy Metabolism AMPK Signaling Pathway

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Transcript of AMPK Signaling Pathway - media.cellsignal.com · Phospho-AMPKα (Thr172) Sandwich ELISA Kit #9839...

www.cellsignal.com/ampkFor pathway key and background please visit:

Other RTKs-AdrenergicReceptor

AMPK

PFKFB3

LKB1MO25STRAD

Adiponectin Receptor

Leptin

PKA Ras

Rheb

PLC Gq

SirT1

GS TBC1D1

ACC

CPT1Malonyl

CoA

CaMKK

HMG-CoAReductase

FIP200

TSC2TSC1

PGC1

Insulin

SREBP-1

Fatty AcidSynthase

HSL

ATGL

Akt

B-Raf

Erk

p90RSK

PI3K

InsulinReceptor

p70S6K

4E-BP1

Beclin

mTOR GLRaptor

ULK1

PI3KClass III

ATG13

Atg14

p300

HNF4

TORC2/CRTC2

CREB

FoxO

HDAC7HDAC5HDAC4

CRY1

Glut4vesicle

Glut1vesicle

Sterol/IsoprenoidSynthesis

Fatty AcidOxidation

Lipolysis

Glycolysis

GlycogenSynthesis

[cAMP]

Low Glucose,Hypoxia, Ischemia,

Heat ShockAICAR

ExerciseBrain

[Ca2+]

HistamineThrombin

GlutTrafficking

Glut4Glut1

Autophagy

Lipogenesis

AMP + ADPATP

Gluconeogenesis

Glucose

Glut Translocation

Microtubules

ActinDynamics

MBS85MYPT1

TXNIP

MyosinLight

Chain2

CLIP170Tau

Cytoskeletal Signaling

Acute Regulation of M

etabolism

Transcriptio

nal Contr

ol of M

etabol

ism

Prot

ein

Met

abol

ism a

nd A

utop

hagy

Energy Metabolism

AMPK Signaling Pathway

www.cellsignal.com/ampkFor Research Use Only. Not For Use In Diagnostic Procedures.

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YENG

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FM10

3ENG

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2015 Cell Signaling Technology, Inc. Cell Signaling Technology, CST, PathScan, PhosphoPlus, and XP are trademarks of Cell Signaling Technology, Inc. All other trademarks are the property of their respective owners.

Key Products for Energy Metabolism

ANTIBODIES APPLICATIONS REACTIVITY#3676 Acetyl-CoA Carboxylase (C83B10) Rabbit mAb WB, IHC-P, IF-IC, F H, M, R, Hm

#11818 Phospho-Acetyl-CoA Carboxylase (Ser79) (D7D11) Rabbit mAb WB, IP, IHC-P, IF-IC H, M, R

#5831 AMPK (D5A2) Rabbit mAb WB, IP H, M, R, Mk, B

#2532 AMPK Antibody WB, IP H, M, R, Hm, Mk

#2793 AMPK (F6) Mouse mAb WB H, M, R, Hm, Mk

#2535 Phospho-AMPK (Thr172) (40H9) Rabbit mAb WB, IP, IHC-P H, M, R, Hm, Mk, Dm, Sc, (C, Z, B, Pg)

#2795 AMPK1 Antibody WB H, Mk, (Pg)

#2757 AMPK2 Antibody WB, IP H, Mk

#4185 Phospho-AMPK1 (Ser485)/AMPK2 (Ser491) Antibody WB H, M, R, Mk

#4150 AMPK1/2 (57C12) Rabbit mAb WB, IHC-P, IF-IC, F H, M, R, Hm, Mk

#4181 Phospho-AMPK1 (Ser108) Antibody WB, IP H, M, R, Mk

#4458 ARK5 Antibody WB H, M, R, Mk, (B)

#8057 ASCT2 (D7C12) Rabbit mAb WB, IP, IF-IC, F H, M, Mk

#4332 ATP-Citrate Lyase Antibody WB H, M, R, Mk

#4331 Phospho-ATP-Citrate Lyase (Ser455) Antibody WB, IP H, M, (R)

#12980 Catalase (D4P7B)XP Rabbit mAb WB, IP, IF-IC H

#14309 Citrate Synthase (D7V8B) Rabbit mAb WB, IF-IC H, M, R, Hm, Mk

#4850 COX IV (3E11) Rabbit mAb WB, IP, IHC-P, IHC-F, IF-IC, F H, R, Mk, Z, B, Pg

#11967 COX IV (4D11-B3-E8) Mouse mAb WB, IP, IHC-P, IF-IC H, M, R, Mk

#12963 Cytochrome c (6H2.B4) Mouse mAb IP, IF-IC H, M, R

#12362 DLAT (4A4-B6-C10) Mouse mAb WB, IP, IF-IC H, Mk

#3838 EAAT2 Antibody WB, IF-F M, R, (H)

#8137 IDH1 (D2H1) Rabbit mAb WB, IP, IF-IC, F H, M, R, Mk

#12652 IDH2 (D7H6Q) Rabbit mAb WB, IP H, M, R, Mk

#3582 LDHA (C4B5) Rabbit mAb WB, IHC-P, IF-IC H, Mk

#8176 Phospho-LDHA (Tyr10) Antibody WB H, M, R

#3047 LKB1 (D60C5) Rabbit mAb WB H, M, R, Mk

#13031 LKB1 (D60C5F10) Rabbit mAb (IHC Formulated) IHC-P H, M, Mk

#3482 Phospho-LKB1 (Ser428) (C67A3) Rabbit mAb WB H, M, R, Mk

#2716 MO25/CAB39 (C49D8) Rabbit mAb WB, IP H, M, R, Mk

#2183 NFAT3 (23E6) Rabbit mAb WB H, M, R

#12721 NRF2 (D1Z9C)XP Rabbit mAb WB, IP, F, ChIP H, M, Mk

#3820 PDHK1 (C47H1) Rabbit mAb WB, IP H, M, R, Mk, (B)

#3205 Pyruvate Dehydrogenase (C54G1) Rabbit mAb WB, IHC-P H, M, R, Mk

#11998 SDHA (D6J9M) XP Rabbit mAb WB, IP, IHC-P, IF-IC H, M, R, Hm, Mk

#8071 Succinyl-CoA Synthetase (D8A11) Rabbit mAb WB, IP, IF-IC H, M, R, Mk

#13002 Syntaxin 1A Antibody WB H, M, R, (Mk)

#8076 TFAM (D5C8) Rabbit mAb WB, IP, IF-IC H

#14670 UCP1(D9D6X) Rabbit mAb WB, IP M, (H)

#13508 VAMP2 (D6O1A) Rabbit mAb WB, IP, IHC-P, IF-F, IF-IC H, M, R

#12331 VGLUT1 Antibody WB M, R, (H)

#12691 xCT/SLC7A11 (D2M7A) Rabbit mAb WB, IP H, (Mk)

RELATED KITS AND ASSAYS#7959 PathScan Phospho-AMPK (Thr172) Sandwich ELISA Kit

#9839 AMPK Subunit Antibody Sampler Kit

#8660 PPAR Regulated Fatty Acid Metabolism Antibody Sampler Kit

#8674 Mitochondrial Marker Antibody Sampler Kit

#5132 PhosphoPlus LKB1 (Ser428) Antibody Duet

kDa

Phospho-AMPK(Thr172)

AMPK

Oligomycin

100

80

60

50

40

100

80

60

50

40 +

Phospho-AMPK (Thr172) (40H9) Rabbit mAb #2535: WB analysis of extracts from 293 cells, untreated (-) or treated with Oligomycin #9996 (0.5 M, 30 min; +), using #2535 (upper) or AMPK Antibody #2532 (lower).

kDa

LKB1

CAKI

COS

L929

PC12

140

10080

6050

40

LKB1 (D60C5) Rabbit mAb #3047: WB analysis of extracts from various cell lines using #3047.

Visit www.cellsignal.com/ampklist for full listing of antibodies, related kits, and reagents.

COX IV (3E11) Rabbit mAb #4850: Confocal IF analysis of HeLa cells using #4850 (green). Actin filaments were labeled with Alexa Fluor 555 Phalloidin #8953 (red). Blue pseudocolor = DRAQ5 #4084 (fluorescent DNA dye).

SDHA (D6J9M) XP Rabbit mAb #11998: IHC analysis of paraffin-embedded human colon carcinoma using #11998 in the presence of control peptide (top) or antigen-specific peptide (bottom).

APPLICATIONS & REACTIVITY KEY: WB Western Blotting / IP Immunoprecipitation / IHC Immunohistochemistry / IF Immunofluorescence / F Flow Cytometry / ChIP Chromatin Immunoprecipitation / -IC Immunocytochemistry / -P Paraffin / -F Frozen / E-P Peptide ELISA / H human / M mouse / R rat / Hm hamster / Mk monkey / C chicken / Mi mink / Dm D. melanogaster / X Xenopus / Z zebra fish / B bovine / Dg dog / Pg pig / Sc S. cerevisiae / All all species expected / ( ) 100% sequence homology