Global Regulatory Mechanisms FIND CHAP TOC

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Global Regulatory Mechanisms 13 FIND CHAP TOC ������������������ �� Table 13.1

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chap_13

























1.0
0.1
Time
Glucose
Galactose
IIAGlc
XXX
P~
P~
Glucose
Glucose-6-P
Glycolysis
IIGlc
β′β
G –70 –55 –35 –10 +1
T G G T T A GA C T C A
C
A
A C C A A T CT G A G T G mRNA
C RNA Pol interaction region
+1–61.5
I1 I2
–35 hexamer
–10 hexamer
RNA Polymerase
σCRP AraC
Figure 13.4
CAP
cAMP
CAP site
RNA Pol interactions Operator
–35 region –10 region
G T G A G T T
CAP site
C A C
L8
T C A A T C G A G T G
T A T G T T G T G T GG A +1
A T T
A T A C
Class IIIClass IIClass I
A A C A C A C C T T A A
–10 –35
AA TT
Low NH3
NH3 + α-ketoglutarate glutamate Glutamate dehydrogenase
NH3 + glutamate
Figure 13.7
σ54
–140
P
Periplasm
Cytoplasm
D
N
D H
p1 p2 glnA ntrB ntrCp3
p3 σ70
p1 σ70
p2 σ54
–35 σ70 promoter
T T A
A T A A T A T A T T A
+1
–24 –12 +1
C T GG X A G A C C X T
T T A
T G C A A A C G T
T C T C X C C C T T G A A A G A G X GGG A A C T T
–10
C C C C A T X T A GGGG T A X A T
Figure 13.10
N C










RNA Pol
NtrC binding
UAS Promoter
σ54 binding
G C A C C A N3 T GG T G C
T T GG C A C A G A T T T C G C T
C G T GG T N3 A C C A C G
σ54
ON














1 3 2
OFF
ompT toxR toxS ctxA ctxBtcpP tcpH tcpA–F toxT
OmpT
ToxS
Cytoplasm
bvgSbvgA G BvgS+GGGGG C C C C
G C+
C C
G C–
bvgSbvgA G BvgS+GGGGG C C C C C C
Figure 13.14
σ32
p1
σ24
Global Regulatory Mechanisms13 FIND CHAP TOC
thrU tyrU glyT thrT tufB rplK rplA rplJ rplL rpoB rpoC
Figure 13.16
mRNA
rplArplK
One copy Gene A
EF-Tu
RelA
ppGpp
ppGpp
C U G U U U A A A C A C A A A
A A A
U U
Ribosome stalls
Gln NH2
C U G U U U A A A A A A
Phe
Glass slide
Affix DNA
More expression in
Somewhat more expression in
B Athan
mix