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CYTOKINES  CYTOKINES  

MEFST, Medical studies in EnglishMEFST, Medical studies in English

2012/13.2012/13.

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What are cytokines?What are cytokines?

Interleukins (IL 1Interleukins (IL 1--35)35) Interpherons (IF type 1,2,3)Interpherons (IF type 1,2,3)

 

  Colony stimulating factors (CSF)Colony stimulating factors (CSF)

Others (TGFOthers (TGF-- ββ, LIF, MIF), LIF, MIF)

ChemokinesChemokines

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General properties of cytokinesGeneral properties of cytokines II

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General properties of cytokines IIGeneral properties of cytokines II

NOT STOREDNOT STORED

HAVE LOCAL AND SYSTEMIC EFFECTSHAVE LOCAL AND SYSTEMIC EFFECTS

ONE STIMULATES SECRETION OF OTHER ONE STIMULATES SECRETION OF OTHER 

HIGH RECEPTOR AFFINITY HIGH RECEPTOR AFFINITY 

CHANGE THE LEVEL OF GENE TRANSCRIPTIONCHANGE THE LEVEL OF GENE TRANSCRIPTION(except chemokines, and sometimes TNF)(except chemokines, and sometimes TNF)

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Cytokine receptor familiesCytokine receptor families

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Innate and adaptive immunityInnate and adaptive immunity

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CytokineCytokine classificationclassification

INNATE IMMUNITY CYTOKINESINNATE IMMUNITY CYTOKINES

 

 

HEMATOPOIETIC CYTOKINESHEMATOPOIETIC CYTOKINES

IMMUNOSUPPRESSIVE CYTOKINESIMMUNOSUPPRESSIVE CYTOKINES

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INNATE IMMUNITYINNATE IMMUNITYCYTOKINESCYTOKINES

Secreted by macrophagesSecreted by macrophages Stimmulated by LPS, nucleic acids,Stimmulated by LPS, nucleic acids,

Those are:Those are:

TNFTNF--αααααααα , IL , IL--1, IL1, IL--12, IF12, IFαααααααα ii ββββββββ

ILIL--6, IL6, IL--8, IL8, IL--18, IL18, IL--23, IL23, IL--27,…27,…

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Tumor necrosis factorTumor necrosis factor

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TNF receptor cell sygnalingTNF receptor cell sygnaling

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ILIL--1 family1 family

 A complex network of proinflamatory A complex network of proinflamatorycytokinescytokines

  -- --  ,, , ..., ...

FFunction simmilar to TNFunction simmilar to TNF

Do NOT lead to apoptosis or septicDo NOT lead to apoptosis or septic

shock shock  Syntesized from a stored precursorSyntesized from a stored precursor

proteinprotein

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Other innate immunity cytokinesOther innate immunity cytokines

ILIL--1010 Inhibits ILInhibits IL--12 and TNF12 and TNF--αααααααα production, as well asproduction, as well as

MHCMHC – –class II (immunosuppression)class II (immunosuppression)

ILIL--66 Stimulates acuteStimulates acute--phase proteins andphase proteins and

lymphocite T/B growth (adaptive immunity)lymphocite T/B growth (adaptive immunity)

ILIL--1515 Stimulates NK cell proliferationStimulates NK cell proliferation

ILIL--8, IL8, IL--18, IL18, IL--23, IL23, IL--2727

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 Adaptive immunity cytokines Adaptive immunity cytokines

Secreted by TSecreted by T--lymphocyteslymphocytes Stimulated by antigenesStimulated by antigenes

Those are:Those are:

ILIL--2, IL2, IL--4, IL4, IL--5, IF5, IF--γγ

ILIL--6, IL6, IL--11, IL11, IL--13, IL13, IL--17,…17,…

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(Th)

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(Th-2)

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ILIL--55

 Activates eosinophiles Activates eosinophiles

Stimulates the production of Th2Stimulates the production of Th2

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(TL, NK, makrofagi)

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Immunosuppression cytokinesImmunosuppression cytokines

produced by CD4+Tproduced by CD4+T--ly, macrophagesly, macrophages

Suppress macrophages and Th1Suppress macrophages and Th1

Those are:Those are:

 TGFTGF--ββββββββ , IL , IL--1010 ILIL--4, IL4, IL--13,…13,…

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TGFTGF--ßß

Inhibits lymphocyte T and macrophagesInhibits lymphocyte T and macrophages

Stimulates IgA secretionStimulates IgA secretion

Stimulates production of extracellularStimulates production of extracellularmatrixmatrix (healing and reparation)(healing and reparation)

ILIL--1010 Inhibits macrophagesInhibits macrophages

IILL--44 andand ILIL--1313 Inhibit cellInhibit cell--mediated and stimulatemediated and stimulate

humoral immunityhumoral immunity

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Th lymphocytes

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Th1 cell functionTh1 cell function

Proliferation anddifferentiation (IFN-γ, IL-12)

Th2 ll f tiTh2 ll f ti

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Th2 cell functionTh2 cell function

I L - 4I L - 1 0I L - 1 3

Proliferation anddifferentiation (IL-4)

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Th-17lymphocytes

Proliferation anddifferentiation(TGF-β, IL-6, IL-23)

Discovered in 2009.Role in:- host deffense against patogene- tissue inflammation

- autoimmune diseases

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HEMATOPOIETIC CYTOKINESHEMATOPOIETIC CYTOKINES

Produced by bone marrow cells andProduced by bone marrow cells andleukocytesleukocytes

Those are:Those are:

CSFCSF

ILIL--77 ILIL--3 (multi CSF)3 (multi CSF)

ILIL--1, IL1, IL--5, IL5, IL--6, erythropoietin, …6, erythropoietin, …

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