BZ-X800 Quick Operation Guide 96M14856 · For a large dish or oversized slide: Set large dishes or...

6
Top panel OPEN Power ON Main power ON BZ-X800 compatible lenses This list shows the BZ-X800 lenses and their compatible container types. The lens currently used is displayed. Lens name Magnification Phase contrast Oil- immersion Slide/Glass bottom (Thickness 0.17 mm) WD (mm) 8.50 Plastic container (Thickness 1 mm or more) CFI Plan Apo λ 2x CFI Plan Apo λ 4x CFI Plan Apo λ 10x CFI Plan Fluor 20x CFI Plan Apo λ 20x CFI Plan Apo λ 40x 2x 4x 10x 20x 20x 40x 4x 10x 20x 40x 60x 100x S PL FL ELWD ADM 20xC S PL FL ELWD ADM 40xC CFI Plan Fluor DL 4x CFI Plan Fluor DL 10x CFI Plan Apo λ 60xH CFI Plan Apo λ 100xH Basic Lenses Phase Lenses Oil Lenses 20.00 4.00 1.00 2.10 0.21 0.13 0.13 16.50 15.20 8.2 to 6.9 3.6 to 2.8 Lens Slide glass Cover glass Set the specimen on the sample holder, and install the sample holder to the holder frame. Attach the holder frame to the XY stage. For slides: Set slides on the sample holder 468.833 mm (slide × 3). For 35 mm dishes: Set 35 mm dishes on the sample holder (35 mm dish × 3). For a well plate or flask: Set a multi-well plate or a flask directly on the holder frame. For a large dish or oversized slide: Set large dishes or oversized slides on the regular versatile holder. Note: When mounting specimens, invert slides so the cover glass faces downward. Power ON Mounting specimens Changing lenses and magnifications Quick Operation Guide BZ-X800 1 Click [Adjust Lens]. 1 Click the icon of the lens to adjust. 2 Open the top panel or front panel, and then apply oil or adjust the correction ring. 3 When the adjustment is complete, click [OK]. 4 2 Type of holder Lens adjustment When applying oil or adjusting a correction ring, use the lens adjustment function. Click the lens button. 1 1 Click the icon of the lens to adjust. 2 Lens change

Transcript of BZ-X800 Quick Operation Guide 96M14856 · For a large dish or oversized slide: Set large dishes or...

Page 1: BZ-X800 Quick Operation Guide 96M14856 · For a large dish or oversized slide: Set large dishes or oversized slides on the regular versatile holder. Note: When mounting specimens,

Top panel OPEN

Power ON

Main power ON

BZ-X800 compatible lenses This list shows the BZ-X800 lenses and their compatible container types.

The lens currently used is

displayed.

Lens name Magnification Phasecontrast

Oil-immersion

Slide/Glass bottom(Thickness 0.17 mm)

WD(mm)

8.50

Plastic container(Thickness 1 mm or more)

CFI Plan Apo λ 2x

CFI Plan Apo λ 4x

CFI Plan Apo λ 10x

CFI Plan Fluor 20x

CFI Plan Apo λ 20x

CFI Plan Apo λ 40x

2x

4x

10x

20x

20x

40x

4x

10x

20x

40x

60x

100x

S PL FLELWD ADM 20xC

S PL FLELWD ADM 40xC

CFI Plan Fluor DL 4x

CFI Plan Fluor DL 10x

CFI Plan Apo λ 60xH

CFI Plan Apo λ 100xH

Basic L

enses

Ph

ase Len

sesO

il Len

ses

20.00

4.00

1.00

2.10

0.21

0.13

0.13

16.50

15.20

8.2 to 6.9

3.6 to 2.8

Lens

Slide glass

Cover glass

Set the specimen on the sample holder, and install the sample holder to the holder frame.

Attach the holder frame to the XY stage.

For slides:Set slides on the sample holder 468.833 mm (slide × 3).

For 35 mm dishes: Set 35 mm dishes on the sample holder (35 mm dish × 3).

For a well plate or flask: Set a multi-well plate or a flask directly on the holder frame.

For a large dish or oversized slide: Set large dishes or oversized slides on the regular versatile holder.

Note:When mounting specimens, invert slides so the cover glass faces downward.1

1

Click [Multi-Color].

2 Click each channel to overlay and individually adjust the brightness and focus.

The overlaid image of the selected channels is displayed on the screen in real time.

3 Click [Capture] to save the images together in a group.

Power ON Mounting specimens

Changing lenses and magnifications

Quick Operation GuideBZ-X800

Advanced FunctionsBZ-X800

Z-stack

Hybrid Cell Count

This function automatically captures multiple focal planes in order to assemble a fully-focused image.

This can convert into numerals by extracting objects by brightness etc. by Analyzer.

If loading the captured image by the analysis software and perform the full focus, multiple images are composited to one image.

11

Click [Adjust Lens].1Click the icon of the lens to adjust.2

Open the top panel or front panel, and then apply oil or adjust the correction ring.

3When the adjustment is complete, click [OK].4

22

Click [Z-Stack].The [Z-Stack] button turns blue.

1 Start the hybrid cell count from Analyzer.

2 Select the type of the image, and click the [Start] button.

3 Set a threshold value of brightness to be extracted.

Furthermore, BZ-X800 can utilize the image cytometer function that can output statistical data of wells by observing multiple samples simultaneously.

4 Adjust the extraction area accordingly.

5 The extraction result can be output by histogram or CSV.

Focus the upper limit of the object, and click the [Set] button on the upper limit.Focus the lower limit as well, and click the [Set] button on the lower limit.

3 Click [Auto] to automatically set the Z-stack pitch.

45

Click [Start Capture] to save the Z-stack as a group.

Type of holder

Lens adjustment

When applying oil or adjusting a correction ring, use the lens adjustment function.

Click the lens button.1

1

Click the icon of the lens to adjust.2

Lens changeAnalyzer

Multi-color capture

3

The enhanced edit and analysis can be made by using Analyzer.

The Ctrl + mouse wheel enables scaling.

An arbitrary length can be displayed by: Inserting → Scale on the menu bar.

The image trimming can be made by specifying area and cutting the image.

Adjusting the level correction can display a dark image to be brighter.

Level correction

Scaling

Scale inserting Trimming

When the field-of-view is moved, click [Recapture All].All channels are updated to display the new location.

2

Note

1058-1 96M14856 Copyright (c) 2018 KEYENCE CORPORATION. All rights reserved.

E-mail: [email protected]

Page 2: BZ-X800 Quick Operation Guide 96M14856 · For a large dish or oversized slide: Set large dishes or oversized slides on the regular versatile holder. Note: When mounting specimens,

Adjusting focusAdjusting exposure (brightness)

Click a channel button to enable observation with the set fluorescence filter and observation method.

In the white balance, the degree of color is adjusted based on the area specified by .

Clicking [Auto-Focus] moves to the focus position automatically. To adjust the focus, right-click the mouse to select [Z Focus], and rotate the mouse wheel.

Switching filters

Click [Mono] or [Color] to switch the mode to monochrome observation (pseudo color) or color observation.

Select [Standard] for fluorescence observation.

Image quality settings:

High ResolutionStandardHigh Sensitivity

1920 × 1440 pixels960 × 720 pixels640 × 480 pixels

BrightDark

Click the buttons to adjust the exposure time. Clicking [Auto] adjusts exposure automatically.

Low photobleach mode:When observing fluorescent samples, set [Low Photobleach] to ON.

NavigationUse this function to display the entire sample, and to quickly move to areas of interest by clicking the desired location on the Navigation image.

1 Display the starting location to register.

2 Click [Navigation].

3

4 Click the location to observe on the navigation screen.

5

Method 2

Method 1

Image stitch (wide-area continuous capture)If the whole image cannot be accommodate, the stitched image can be captured easily.

Click the [Start Capture] button to start the capture.

Specifying the number of images

2

3

Setting the capture range automatically

1 Click [Stitching].

4 If the capture completes, click "Open with the Analysis Software." Or, double-click a group capture information file (*.gci) in the folder with captured images.

5 Loading stitched images with Analyzer starts WideImageViewer.

Basic OperationsBZ-X800 Advanced FunctionsBZ-X800

Select [Set Edge Points] and click [Set] on the edge of the area to capture.

Specify the capture range by setting the

central field-of-view and the total number of

images.

Specify the capture range by setting the

outermost edges, and the number of images

to be captured is calculated automatically.

Select [Set Center and Number of Images] and enter the number of images to capture in the horizontal and vertical directions.

Note: If the bottom surface such as the plate is in focus, click [Focus Up] again.

Tip You can adjust the focus by keeping on pressing the key on the keyboard with moving the mouse wheel as well.

[Ctrl] + [Shift] key + wheel[Ctrl] key + wheel [Shift] key + wheel

Super rough operationRough operationSlight operation

Clicking on the stage view performs the XY movement.

The stage moves to the pressed arrow direction. Pressing the [C] key moves to the original point.

Double-clicking on the observation screen moves the position to the center of the screen.The XY movement can be made by dragging on the screen as well.

Using the well plate, the stage moves to the center of the well by double-clicking.

X-Y movement

Black balance (Fluorescence observation)

White balance (Brightfield observation)

Drag the rectangle frame on the background to be black on the screen, and click the set.

Black balance/White balance

Observation of fluorescence signals is much clearer after using Black Balance.

The tilt correction can be made based on the Z-axis location of the specified point in the edge specification.If the influence from the tilt is big because of the shallow depth such as by high magnification lens, using the edge specification can capture images better.

An image of which resolution exceeds 4080 x 3072 cannot be handled in the analysis software, so the resolution is made small.If you want to save it with the same resolution as the original, save it as a KTF file by Wide Image Viewer.

The file of KTF is the original KEYENCE file format which handles a high resolution image with high speed.

Click [Load]

Click [Start Stitching]

Wide Image Viewer opens

1

2

5

3

4

Sectioning capture

1 Adjust the position and focus of the image on the observation screen, and then click [Sectioning].

1

2

4

5

2 Select [1D slit] for the type and set the slide bar at the left end (10). If the grid pattern does not display as clear, distinct lines, move the slide bar to the right.

3 Click [Auto] for Brightness. Saturated pixels are displayed in the set color. Confirm that the image contains no saturated pixels prior to capture.

4 Click [Preview] to view the sectioning image.

5 Click [Capture] to save the image.

Setting capture conditions for separate locations

1

The Multi-Point function allows up to 999 different locations to be sequentially captured and saved using individual capture settings (including magnification, channel, observation method, exposure time, etc.).

2 Select the [Use Individual Setting] check box, select the desired area for registration on the stage view, and click the [Set] button.

Capture locations are displayed as points on the stage view.

The capture condition is displayed if clicking [Setting].

1 Click [Multi-Point].The [Multi-Point] button turns blue.

3 Click [Start Capture] to automatically capture all set locations.

Note

Switching CCDs

Click [Add] on the navigation screen. Image capture begins with the specified position in the center, and creates the navigation image.

Clicking [Set] for Point Memo allows up to 30 capture locations to be registered.The registered locations are also displayed on the navigation screen.

Page 3: BZ-X800 Quick Operation Guide 96M14856 · For a large dish or oversized slide: Set large dishes or oversized slides on the regular versatile holder. Note: When mounting specimens,

Top panel OPEN

Power ON

Main power ON

BZ-X800 compatible lenses This list shows the BZ-X800 lenses and their compatible container types.

The lens currently used is

displayed.

Lens name Magnification Phasecontrast

Oil-immersion

Slide/Glass bottom(Thickness 0.17 mm)

WD(mm)

8.50

Plastic container(Thickness 1 mm or more)

CFI Plan Apo λ 2x

CFI Plan Apo λ 4x

CFI Plan Apo λ 10x

CFI Plan Fluor 20x

CFI Plan Apo λ 20x

CFI Plan Apo λ 40x

2x

4x

10x

20x

20x

40x

4x

10x

20x

40x

60x

100x

S PL FLELWD ADM 20xC

S PL FLELWD ADM 40xC

CFI Plan Fluor DL 4x

CFI Plan Fluor DL 10x

CFI Plan Apo λ 60xH

CFI Plan Apo λ 100xH

Basic L

enses

Ph

ase Len

sesO

il Len

ses

20.00

4.00

1.00

2.10

0.21

0.13

0.13

16.50

15.20

8.2 to 6.9

3.6 to 2.8

Lens

Slide glass

Cover glass

Set the specimen on the sample holder, and install the sample holder to the holder frame.

Attach the holder frame to the XY stage.

For slides:Set slides on the sample holder 468.833 mm (slide × 3).

For 35 mm dishes: Set 35 mm dishes on the sample holder (35 mm dish × 3).

For a well plate or flask: Set a multi-well plate or a flask directly on the holder frame.

For a large dish or oversized slide: Set large dishes or oversized slides on the regular versatile holder.

Note:When mounting specimens, invert slides so the cover glass faces downward.1

1

Click [Multi-Color].

2 Click each channel to overlay and individually adjust the brightness and focus.

The overlaid image of the selected channels is displayed on the screen in real time.

3 Click [Capture] to save the images together in a group.

Power ON Mounting specimens

Changing lenses and magnifications

Quick Operation GuideBZ-X800

Advanced FunctionsBZ-X800

Z-stack

Hybrid Cell Count

This function automatically captures multiple focal planes in order to assemble a fully-focused image.

This can convert into numerals by extracting objects by brightness etc. by Analyzer.

If loading the captured image by the analysis software and perform the full focus, multiple images are composited to one image.

11

Click [Adjust Lens].1Click the icon of the lens to adjust.2

Open the top panel or front panel, and then apply oil or adjust the correction ring.

3When the adjustment is complete, click [OK].4

22

Click [Z-Stack].The [Z-Stack] button turns blue.

1 Start the hybrid cell count from Analyzer.

2 Select the type of the image, and click the [Start] button.

3 Set a threshold value of brightness to be extracted.

Furthermore, BZ-X800 can utilize the image cytometer function that can output statistical data of wells by observing multiple samples simultaneously.

4 Adjust the extraction area accordingly.

5 The extraction result can be output by histogram or CSV.

Focus the upper limit of the object, and click the [Set] button on the upper limit.Focus the lower limit as well, and click the [Set] button on the lower limit.

3 Click [Auto] to automatically set the Z-stack pitch.

45

Click [Start Capture] to save the Z-stack as a group.

Type of holder

Lens adjustment

When applying oil or adjusting a correction ring, use the lens adjustment function.

Click the lens button.1

1

Click the icon of the lens to adjust.2

Lens changeAnalyzer

Multi-color capture

3

The enhanced edit and analysis can be made by using Analyzer.

The Ctrl + mouse wheel enables scaling.

An arbitrary length can be displayed by: Inserting → Scale on the menu bar.

The image trimming can be made by specifying area and cutting the image.

Adjusting the level correction can display a dark image to be brighter.

Level correction

Scaling

Scale inserting Trimming

When the field-of-view is moved, click [Recapture All].All channels are updated to display the new location.

2

Note

1058-1 96M14856 Copyright (c) 2018 KEYENCE CORPORATION. All rights reserved.

E-mail: [email protected]

Page 4: BZ-X800 Quick Operation Guide 96M14856 · For a large dish or oversized slide: Set large dishes or oversized slides on the regular versatile holder. Note: When mounting specimens,

Adjusting focusAdjusting exposure (brightness)

Click a channel button to enable observation with the set fluorescence filter and observation method.

In the white balance, the degree of color is adjusted based on the area specified by .

Clicking [Auto-Focus] moves to the focus position automatically. To adjust the focus, right-click the mouse to select [Z Focus], and rotate the mouse wheel.

Switching filters

Click [Mono] or [Color] to switch the mode to monochrome observation (pseudo color) or color observation.

Select [Standard] for fluorescence observation.

Image quality settings:

High ResolutionStandardHigh Sensitivity

1920 × 1440 pixels960 × 720 pixels640 × 480 pixels

BrightDark

Click the buttons to adjust the exposure time. Clicking [Auto] adjusts exposure automatically.

Low photobleach mode:When observing fluorescent samples, set [Low Photobleach] to ON.

NavigationUse this function to display the entire sample, and to quickly move to areas of interest by clicking the desired location on the Navigation image.

1 Display the starting location to register.

2 Click [Navigation].

3

4 Click the location to observe on the navigation screen.

5

Method 2

Method 1

Image stitch (wide-area continuous capture)If the whole image cannot be accommodate, the stitched image can be captured easily.

Click the [Start Capture] button to start the capture.

Specifying the number of images

2

3

Setting the capture range automatically

1 Click [Stitching].

4 If the capture completes, click "Open with the Analysis Software." Or, double-click a group capture information file (*.gci) in the folder with captured images.

5 Loading stitched images with Analyzer starts WideImageViewer.

Basic OperationsBZ-X800 Advanced FunctionsBZ-X800

Select [Set Edge Points] and click [Set] on the edge of the area to capture.

Specify the capture range by setting the

central field-of-view and the total number of

images.

Specify the capture range by setting the

outermost edges, and the number of images

to be captured is calculated automatically.

Select [Set Center and Number of Images] and enter the number of images to capture in the horizontal and vertical directions.

Note: If the bottom surface such as the plate is in focus, click [Focus Up] again.

Tip You can adjust the focus by keeping on pressing the key on the keyboard with moving the mouse wheel as well.

[Ctrl] + [Shift] key + wheel[Ctrl] key + wheel [Shift] key + wheel

Super rough operationRough operationSlight operation

Clicking on the stage view performs the XY movement.

The stage moves to the pressed arrow direction. Pressing the [C] key moves to the original point.

Double-clicking on the observation screen moves the position to the center of the screen.The XY movement can be made by dragging on the screen as well.

Using the well plate, the stage moves to the center of the well by double-clicking.

X-Y movement

Black balance (Fluorescence observation)

White balance (Brightfield observation)

Drag the rectangle frame on the background to be black on the screen, and click the set.

Black balance/White balance

Observation of fluorescence signals is much clearer after using Black Balance.

The tilt correction can be made based on the Z-axis location of the specified point in the edge specification.If the influence from the tilt is big because of the shallow depth such as by high magnification lens, using the edge specification can capture images better.

An image of which resolution exceeds 4080 x 3072 cannot be handled in the analysis software, so the resolution is made small.If you want to save it with the same resolution as the original, save it as a KTF file by Wide Image Viewer.

The file of KTF is the original KEYENCE file format which handles a high resolution image with high speed.

Click [Load]

Click [Start Stitching]

Wide Image Viewer opens

1

2

5

3

4

Sectioning capture

1 Adjust the position and focus of the image on the observation screen, and then click [Sectioning].

1

2

4

5

2 Select [1D slit] for the type and set the slide bar at the left end (10). If the grid pattern does not display as clear, distinct lines, move the slide bar to the right.

3 Click [Auto] for Brightness. Saturated pixels are displayed in the set color. Confirm that the image contains no saturated pixels prior to capture.

4 Click [Preview] to view the sectioning image.

5 Click [Capture] to save the image.

Setting capture conditions for separate locations

1

The Multi-Point function allows up to 999 different locations to be sequentially captured and saved using individual capture settings (including magnification, channel, observation method, exposure time, etc.).

2 Select the [Use Individual Setting] check box, select the desired area for registration on the stage view, and click the [Set] button.

Capture locations are displayed as points on the stage view.

The capture condition is displayed if clicking [Setting].

1 Click [Multi-Point].The [Multi-Point] button turns blue.

3 Click [Start Capture] to automatically capture all set locations.

Note

Switching CCDs

Click [Add] on the navigation screen. Image capture begins with the specified position in the center, and creates the navigation image.

Clicking [Set] for Point Memo allows up to 30 capture locations to be registered.The registered locations are also displayed on the navigation screen.

Page 5: BZ-X800 Quick Operation Guide 96M14856 · For a large dish or oversized slide: Set large dishes or oversized slides on the regular versatile holder. Note: When mounting specimens,

Adjusting focusAdjusting exposure (brightness)

Click a channel button to enable observation with the set fluorescence filter and observation method.

In the white balance, the degree of color is adjusted based on the area specified by .

Clicking [Auto-Focus] moves to the focus position automatically. To adjust the focus, right-click the mouse to select [Z Focus], and rotate the mouse wheel.

Switching filters

Click [Mono] or [Color] to switch the mode to monochrome observation (pseudo color) or color observation.

Select [Standard] for fluorescence observation.

Image quality settings:

High ResolutionStandardHigh Sensitivity

1920 × 1440 pixels960 × 720 pixels640 × 480 pixels

BrightDark

Click the buttons to adjust the exposure time. Clicking [Auto] adjusts exposure automatically.

Low photobleach mode:When observing fluorescent samples, set [Low Photobleach] to ON.

NavigationUse this function to display the entire sample, and to quickly move to areas of interest by clicking the desired location on the Navigation image.

1 Display the starting location to register.

2 Click [Navigation].

3

4 Click the location to observe on the navigation screen.

5

Method 2

Method 1

Image stitch (wide-area continuous capture)If the whole image cannot be accommodate, the stitched image can be captured easily.

Click the [Start Capture] button to start the capture.

Specifying the number of images

2

3

Setting the capture range automatically

1 Click [Stitching].

4 If the capture completes, click "Open with the Analysis Software." Or, double-click a group capture information file (*.gci) in the folder with captured images.

5 Loading stitched images with Analyzer starts WideImageViewer.

Basic OperationsBZ-X800 Advanced FunctionsBZ-X800

Select [Set Edge Points] and click [Set] on the edge of the area to capture.

Specify the capture range by setting the

central field-of-view and the total number of

images.

Specify the capture range by setting the

outermost edges, and the number of images

to be captured is calculated automatically.

Select [Set Center and Number of Images] and enter the number of images to capture in the horizontal and vertical directions.

Note: If the bottom surface such as the plate is in focus, click [Focus Up] again.

Tip You can adjust the focus by keeping on pressing the key on the keyboard with moving the mouse wheel as well.

[Ctrl] + [Shift] key + wheel[Ctrl] key + wheel [Shift] key + wheel

Super rough operationRough operationSlight operation

Clicking on the stage view performs the XY movement.

The stage moves to the pressed arrow direction. Pressing the [C] key moves to the original point.

Double-clicking on the observation screen moves the position to the center of the screen.The XY movement can be made by dragging on the screen as well.

Using the well plate, the stage moves to the center of the well by double-clicking.

X-Y movement

Black balance (Fluorescence observation)

White balance (Brightfield observation)

Drag the rectangle frame on the background to be black on the screen, and click the set.

Black balance/White balance

Observation of fluorescence signals is much clearer after using Black Balance.

The tilt correction can be made based on the Z-axis location of the specified point in the edge specification.If the influence from the tilt is big because of the shallow depth such as by high magnification lens, using the edge specification can capture images better.

An image of which resolution exceeds 4080 x 3072 cannot be handled in the analysis software, so the resolution is made small.If you want to save it with the same resolution as the original, save it as a KTF file by Wide Image Viewer.

The file of KTF is the original KEYENCE file format which handles a high resolution image with high speed.

Click [Load]

Click [Start Stitching]

Wide Image Viewer opens

1

2

5

3

4

Sectioning capture

1 Adjust the position and focus of the image on the observation screen, and then click [Sectioning].

1

2

4

5

2 Select [1D slit] for the type and set the slide bar at the left end (10). If the grid pattern does not display as clear, distinct lines, move the slide bar to the right.

3 Click [Auto] for Brightness. Saturated pixels are displayed in the set color. Confirm that the image contains no saturated pixels prior to capture.

4 Click [Preview] to view the sectioning image.

5 Click [Capture] to save the image.

Setting capture conditions for separate locations

1

The Multi-Point function allows up to 999 different locations to be sequentially captured and saved using individual capture settings (including magnification, channel, observation method, exposure time, etc.).

2 Select the [Use Individual Setting] check box, select the desired area for registration on the stage view, and click the [Set] button.

Capture locations are displayed as points on the stage view.

The capture condition is displayed if clicking [Setting].

1 Click [Multi-Point].The [Multi-Point] button turns blue.

3 Click [Start Capture] to automatically capture all set locations.

Note

Switching CCDs

Click [Add] on the navigation screen. Image capture begins with the specified position in the center, and creates the navigation image.

Clicking [Set] for Point Memo allows up to 30 capture locations to be registered.The registered locations are also displayed on the navigation screen.

Page 6: BZ-X800 Quick Operation Guide 96M14856 · For a large dish or oversized slide: Set large dishes or oversized slides on the regular versatile holder. Note: When mounting specimens,

Top panel OPEN

Power ON

Main power ON

BZ-X800 compatible lenses This list shows the BZ-X800 lenses and their compatible container types.

The lens currently used is

displayed.

Lens name Magnification Phasecontrast

Oil-immersion

Slide/Glass bottom(Thickness 0.17 mm)

WD(mm)

8.50

Plastic container(Thickness 1 mm or more)

CFI Plan Apo λ 2x

CFI Plan Apo λ 4x

CFI Plan Apo λ 10x

CFI Plan Fluor 20x

CFI Plan Apo λ 20x

CFI Plan Apo λ 40x

2x

4x

10x

20x

20x

40x

4x

10x

20x

40x

60x

100x

S PL FLELWD ADM 20xC

S PL FLELWD ADM 40xC

CFI Plan Fluor DL 4x

CFI Plan Fluor DL 10x

CFI Plan Apo λ 60xH

CFI Plan Apo λ 100xH

Basic L

enses

Ph

ase Len

sesO

il Len

ses

20.00

4.00

1.00

2.10

0.21

0.13

0.13

16.50

15.20

8.2 to 6.9

3.6 to 2.8

Lens

Slide glass

Cover glass

Set the specimen on the sample holder, and install the sample holder to the holder frame.

Attach the holder frame to the XY stage.

For slides:Set slides on the sample holder 468.833 mm (slide × 3).

For 35 mm dishes: Set 35 mm dishes on the sample holder (35 mm dish × 3).

For a well plate or flask: Set a multi-well plate or a flask directly on the holder frame.

For a large dish or oversized slide: Set large dishes or oversized slides on the regular versatile holder.

Note:When mounting specimens, invert slides so the cover glass faces downward.1

1

Click [Multi-Color].

2 Click each channel to overlay and individually adjust the brightness and focus.

The overlaid image of the selected channels is displayed on the screen in real time.

3 Click [Capture] to save the images together in a group.

Power ON Mounting specimens

Changing lenses and magnifications

Quick Operation GuideBZ-X800

Advanced FunctionsBZ-X800

Z-stack

Hybrid Cell Count

This function automatically captures multiple focal planes in order to assemble a fully-focused image.

This can convert into numerals by extracting objects by brightness etc. by Analyzer.

If loading the captured image by the analysis software and perform the full focus, multiple images are composited to one image.

11

Click [Adjust Lens].1Click the icon of the lens to adjust.2

Open the top panel or front panel, and then apply oil or adjust the correction ring.

3When the adjustment is complete, click [OK].4

22

Click [Z-Stack].The [Z-Stack] button turns blue.

1 Start the hybrid cell count from Analyzer.

2 Select the type of the image, and click the [Start] button.

3 Set a threshold value of brightness to be extracted.

Furthermore, BZ-X800 can utilize the image cytometer function that can output statistical data of wells by observing multiple samples simultaneously.

4 Adjust the extraction area accordingly.

5 The extraction result can be output by histogram or CSV.

Focus the upper limit of the object, and click the [Set] button on the upper limit.Focus the lower limit as well, and click the [Set] button on the lower limit.

3 Click [Auto] to automatically set the Z-stack pitch.

45

Click [Start Capture] to save the Z-stack as a group.

Type of holder

Lens adjustment

When applying oil or adjusting a correction ring, use the lens adjustment function.

Click the lens button.1

1

Click the icon of the lens to adjust.2

Lens changeAnalyzer

Multi-color capture

3

The enhanced edit and analysis can be made by using Analyzer.

The Ctrl + mouse wheel enables scaling.

An arbitrary length can be displayed by: Inserting → Scale on the menu bar.

The image trimming can be made by specifying area and cutting the image.

Adjusting the level correction can display a dark image to be brighter.

Level correction

Scaling

Scale inserting Trimming

When the field-of-view is moved, click [Recapture All].All channels are updated to display the new location.

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Note

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