Anti-acetyl-Histone H2A (Lys5) rabbit pAb ChIP Gradepeptide corresponding to residues surrounding...
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100 μl supplied in liquid form Cat#: Species: Antibody type: Size: Form: This product is produced by immunizing rabbits with a synthetic acetyl peptide corresponding to residues surrounding Lys5 of human histone H2A. Antibodies are purified by protein A-conjugated agarose followed by acetylated histone H2A (Lys5) peptide affinity chromatography. Anti-rabbit secondary antibodies must be used to detect this antibody. 14kDa Molecular Wt. Acetylated H2A (Lys5)-KLH Immunogen Eukaryotes Species Reactivity IP, ChIP Applications P04908 Uniprot ID: # Polyclonal Rabbit GC-116 Anti-acetyl-Histone H2A (Lys5) rabbit pAb ChIP Grade Order: [email protected]; Support: 400-100-1145 [email protected]; Web: www.ptm-biolab.com.cn; PTM Bio, lnc. Figure B: ChIP assay Figure A: Dot blot Sonicated chromatin prepared from 1 x 105 HeLa cells treated with sodium butyrate was subjected to chromatin immunoprecipitation (ChIP) using either 4µl acetyl-histone H2A (Lys5) rabbit pAb or 1 μg of normal rabbit IgG as a negative control. Real time quantitative PCR was performed on immunoprecipitated DNA using primers specific for the human GAPDH promoter and α Satellite. Data are presented as enrichment of each IPed sample relative to total amount of input chromatin at each Dot blotting analysis on indicated amount of acetylated H2A peptide at Lys5 (lane 1), acetylated H2A peptide at Lys9 (lane2), and unmodified H2A peptide at Lys5 (lane 3) using acetyl-histone H2A (Lys5) rabbit pAb. Specificity: Recommended Application: Immunoprecipitation, Chromatin Immunoprecipitation. Recommended antibody dilution: IP: 1:25 ChIP: 1:25. Use at an assay dependent concentration. Optimal dilutions/concentrations should be determined by the end user. Source & Purification: Specificity: Technical Support Academic Platform Storage & Stability: The antibody is kept in PBS with 50% glycerol and 0.01% sodium azide. Upon receipt, please centrifuge the antibody at 12,000 x g for 20 seconds and store the antibody at -20°C. Avoid repeated freeze/thaw cycles. Stable for 12 months from date of receipt. Leave the antibody at room temperature for 2 minutes and gently mixed using pipette before usage. Schematic diagram for reconstitution instructions 12,000 x g 20 seconds Cut the pipette tip Pipet up and down multiple times Histone acetylation alters chromatin structure Scientific Background: Histone post-translational modifications (PTMs) are key mechanisms of epigenetics that modulate chromatin structures, termed as “histone code”. The PTMs on histone including acetylation, methylation, phosphorylation and novel acylations directly affect the accessibility of chromatin to transcription factors and other epigenetic regulators, altering genome stability, gene transcription, etc. Histone acetylation occurs primarily at multiple lysine residues on the amino-ter- minal of core histones, in response to various stimuli and plays vital roles in the regulation of gene expression, DNA damage repair, chromatin dynamics, etc. Mostly, histone H2A is primarily acetylated at Lys5, 9, 15, and 36; H2B is primarily acetylated at Lys5, 12, 15,16,and 20. Histone H3 is primarily acetylated at Lys4, 9, 14, 18, 23, 27, 56,and 79. Histone H4 is primarily acetylated at Lys5, 8, 12, 16, and 20. Histone acetyltransferases (HATs) and histone deacetylases (HDACs) are major regulating factors. This antibody detects histone H2A only when it is acetylated at Lys5, but not the acetylated H2A peptide at Lys9 or the unmodified H2A peptide at Lys5.
Transcript of Anti-acetyl-Histone H2A (Lys5) rabbit pAb ChIP Gradepeptide corresponding to residues surrounding...
100 μl
supplied in liquid form
Cat#:Species:Antibody type:
Size:Form:
This product is produced by immunizing rabbits with a synthetic acetyl peptide corresponding to residues surrounding Lys5 of human histone H2A. Antibodies are purified by protein A-conjugated agarose followed by acetylated histone H2A (Lys5) peptide affinity chromatography.
Anti-rabbit secondary antibodies must be used to detect this antibody.
14kDa
Molecular Wt.
Acetylated H2A (Lys5)-KLH
Immunogen
Eukaryotes
Species Reactivity
IP, ChIP
Applications
P04908Uniprot ID:#Polyclonal
RabbitGC-116
Anti-acetyl-Histone H2A (Lys5) rabbit pAb ChIP Grade
Order: [email protected]; Support: 400-100-1145 [email protected]; Web: www.ptm-biolab.com.cn; PTM Bio, lnc.
Figure B: ChIP assay
Figure A: Dot blot
Sonicated chromatin prepared from 1 x 105 HeLa cells treated with sodium butyrate was subjected to chromatin immunoprecipitation (ChIP) using either 4µl acetyl-histone H2A (Lys5) rabbit pAb or 1 μg of normal rabbit IgG as a negative control. Real time quantitative PCR was performed on immunoprecipitated DNA using primers specific for the human GAPDH promoter and α Satellite. Data are presented as enrichment of each IPed sample relative to total amount of input chromatin at each
Dot blotting analysis on indicated amount of acetylated H2A peptide at Lys5 (lane 1), acetylated H2A peptide at Lys9 (lane2), and unmodified H2A peptide at Lys5 (lane 3) using acetyl-histone H2A (Lys5) rabbit pAb.
Specificity:
Recommended Application: Immunoprecipitation, Chromatin Immunoprecipitation.Recommended antibody dilution: IP: 1:25 ChIP: 1:25.Use at an assay dependent concentration. Optimal dilutions/concentrations should be determined
by the end user.
Source & Purification:
Specificity:
Technical SupportAcademic Platform
Storage & Stability: The antibody is kept in PBS with 50% glycerol and 0.01% sodium azide. Upon receipt, please centrifuge the antibody at 12,000 x g for 20 seconds and store the antibody at -20°C. Avoid repeated freeze/thaw cycles. Stable for 12 months from date of receipt. Leave the antibody at room temperature for 2 minutes and gently mixed using pipette before usage.
Schematic diagram for reconstitution instructions
12,000 x g 20 seconds Cut the pipette tip
Pipet up and down multiple times
Histone acetylation alters chromatin structure
Scientific Background: Histone post-translational modifications (PTMs) are key mechanisms of epigenetics that modulate chromatin structures, termed as “histone code”. The PTMs on histone including acetylation, methylation, phosphorylation and novel acylations directly affect the accessibility of chromatin to transcription factors and other epigenetic regulators, altering genome stability, gene transcription, etc. Histone acetylation occurs primarily at multiple lysine residues on the amino-ter-minal of core histones, in response to various stimuli and plays vital roles in the regulation of gene expression, DNA damage repair, chromatin dynamics, etc. Mostly, histone H2A is primarily acetylated at Lys5, 9, 15, and 36; H2B is primarily acetylated at Lys5, 12, 15,16,and 20. Histone H3 is primarily acetylated at Lys4, 9, 14, 18, 23, 27, 56,and 79. Histone H4 is primarily acetylated at Lys5, 8, 12, 16, and 20. Histone acetyltransferases (HATs) and histone deacetylases (HDACs) are major regulating factors.
This antibody detects histone H2A only when it is acetylated at Lys5, but not the acetylated H2A peptide at Lys9 or the unmodified H2A peptide at Lys5.
