100 μl

supplied in liquid form

Cat#:Species:Antibody type:

Size:Form:

This product is produced by immunizing rabbits with a synthetic acetyl peptide corresponding to residues surrounding Lys5 of human histone H2B. Antibodies are purified by protein A-conjugated agarose followed by acetylated histone H2B (Lys5) peptide affinity chromatography.

Anti-rabbit secondary antibodies must be used to detect this antibody.

14kDa

Molecular Wt.

Acetylated H2B (Lys5)-KLH

Immunogen

Eukaryotes

Species Reactivity

IP, ChIP

Applications

P62807Uniprot ID:#Polyclonal

RabbitGC-117

Anti-acetyl-Histone H2B (Lys5) rabbit pAb ChIP Grade

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Figure B:

Sonicated chromatin prepared from 1x 105 HeLa cells treated with sodium butyrate was subjected to chromatin immunoprecipi-tation (ChIP) using either 4 μl acetyl-histone H2B (Lys5) rabbit pAb or 1 μg of normal rabbit IgG as a negative control. Real time quantitative PCR was performed on immunoprecipitated DNA using primers specific for the human GAPDH promoter and α Satellite. Data are presented as enrichment of each IPed sample relative to total amount of input chromatin at each

Figure A:Specificity:

Recommended Application: Immunoprecipitation, Chromatin Immunoprecipitation.Recommended antibody dilution: IP: 1:25, ChIP: 1:25.

Dot blotting analysis on indicated amount of acetylated H2B peptide at Lys5 (lane 1), butyrylated H2B peptide at Lys5 (lane2), and unmodified H2B peptide at Lys5 (lane 3) using acetyl-histone H2B (Lys5) rabbit pAb.

Source & Purification:

Specificity:

Technical SupportAcademic Platform

Storage & Stability: The antibody is kept in PBS with 50% glycerol and 0.01% sodium azide. Upon receipt, please centrifuge the antibody at 12,000 x g for 20 seconds and store the antibody at -20°C. Avoid repeated freeze/thaw cycles. Stable for 12 months from date of receipt. Leave the antibody at room temperature for 2 minutes and gently mixed using pipette before usage.

Schematic diagram for reconstitution instructions

12,000 x g 20 seconds Cut the pipette tip

Pipet up and down multiple times

Histone acetylation alters chromatin structure

Scientific Background: Histone post-translational modifications (PTMs) are key mechanisms of epigenetics that modulate chromatin structures, termed as “histone code”. The PTMs on histone including acetylation, methylation, phosphorylation and novel acylations directly affect the accessibility of chromatin to transcription factors and other epigenetic regulators, altering genome stability, gene transcription, etc. Histone acetylation occurs primarily at multiple lysine residues on the amino-ter-minal of core histones, in response to various stimuli and plays vital roles in the regulation of gene expression, DNA damage repair, chromatin dynamics, etc. Mostly, histone H2A is primarily acetylated at Lys5, 9, 15, and 36; H2B is primarily acetylated at Lys5, 12, 15,16,and 20. Histone H3 is primarily acetylated at Lys4, 9, 14, 18, 23, 27, 56,and 79. Histone H4 is primarily acetylated at Lys5, 8, 12, 16, and 20. Histone acetyltransferases (HATs) and histone deacetylases (HDACs) are major regulating factors.

This antibody detects histone H2B only when it is acetylated at Lys5, but not the structurally similar butyrylated peptide at Lys5 or the unmodified peptide.