100 μl at

supplied in liquid form

Species reactivity is determined by WB. Kept at -20°C after reconstituted.

Cat#:Species:Antibody type:

Size:Form:

Western blot analysis on 30 ug of crude proteins from HeLa whole cell lysates with (lane2) or without (lane1) treatment of sodium butyrate (30 mM, 4 hours) using Anti-acetyl-Histone H2B (Lys5) mouse pAb (1:2000).

Dot blotting analysis on indicated amount of peptides using Anti-acetyl-Histone H2B (Lys5) mouse pAb. The list of peptides is included in the table.

This product is produced by immunizing mice with a synthetic acetyl peptide corresponding to residues surrounding Lys5 of human histone H2B. Antibodies are purified by protein A-conjugated agarose followed by Acetylated H2B (Lys5) peptide affinity chromatography.

Anti-mouse secondary antibodies must be used to detect this antibody.

14kDa

Molecular Wt.

Acetylated H2B (Lys5)-KLH

Immunogen

Human, Mouse, Rat

Species Reactivity

ELISA, Dot, WB, IP

Applications

P62807Uniprot ID:(1H7)Monoclonal

Mouse

0.19 mg/mlPTM-152

Anti-acetyl-Histone H2B (Lys5) mouse pAb

Order: order@ptm-biolab.com; Support: 400-100-1145 service@ptm-biolab.com; Web: www.ptm-biolab.com.cn; PTM Bio, lnc.

Figure B: Western blot

Figure A: Dot blotSpecificity:

Recommended Application: ELISA, Dot blot, Western blot. Recommended antibody dilution: WB: 1:2000

NOTE: For WB, incubate membrane with diluted antibody in 5% nonfat milk, 1 x TBS, 0.1% Tween-20 for two hours at room temperature with gentle shaking. Prepare working dilution immediately before use. Use at an assay dependent concentration. Optimal dilutions/concentrations should be determined by the end user. Not yet tested in other applica-tions.

Source & Purification:

Technical SupportAcademic Platform

Storage & Stability: The antibody is kept in PBS with 50% glycerol and 0.01% sodium azide. Upon receipt, please centrifuge the antibody at 12,000 x g for 20 seconds and store the antibody at -20°C. Avoid repeated freeze/thaw cycles. Stable for 12 months from date of receipt. Leave the antibody at room temperature for 2 minutes and gently mixed using pipette before usage.

Schematic diagram for reconstitution instructions

12,000 x g 20 seconds Cut the pipette tip

Pipet up and down multiple times

Histone acetylation alters chromatin structure

Scientific Background: Histone post-translational modifications (PTMs) are key mechanisms of epigenetics that modulate chromatin structures, termed as “histone code”. The PTMs on histone including acetylation, methylation, phosphorylation and novel acylations directly affect the accessibility of chromatin to transcription factors and other epigenetic regulators, altering genome stability, gene transcription, etc. Histone acetylation occurs primarily at multiple lysine residues on the amino-ter-minal of core histones, in response to various stimuli and plays vital roles in the regulation of gene expression, DNA damage repair, chromatin dynamics, etc. Mostly, histone H2A is primarily acetylated at Lys5, 9, 15, and 36; H2B is primarily acetylated at Lys5, 12, 15,16,and 20. Histone H3 is primarily acetylated at Lys4, 9, 14, 18, 23, 27, 56,and 79. Histone H4 is primarily acetylated at Lys5, 8, 12, 16, and 20. Histone acetyltransferases (HATs) and histone deacetylases (HDACs) are major regulating factors.

Figure C: IP with PTM-152

0.6mg HeLa whole cell extracts with sodium butyrate treatment (30 mM, 4 hours) was subjected to immunoprecipitation (IP) using Acetyl-Histone H2B (Lys5) mouse mAb (PTM-152) diluted at 1:3 and 1:12 as indicated. And loading 30 μg HeLa cell proteins as input. The western blot analysis was performed using Acetyl-Histone H2B (Lys5) rabbit pAb (PTM-107).