Supplementary Figures

Figure Legends

Suppl. Figure 1. Flow cytometry analysis to monitor apoptosis levels in MCF-7 cells . As control MCF-7 cells grown in complete media were stain just with IP (1st graph) or double stained (IP and Annexin V). 2 μM of Doxorubicin was used as a positive control of apoptosis. Results are shown after treating the cells with doxorubicin in the presence or absence of FBS. The kinetics experiment after serum-deprivation shows an overall increase in apoptosis with increasing time in serum-free media.

 Suppl. Figure 2. IHC detection of secretome proteins in both normal breast tissue and breast cancer tissue obtained from Protein Atlas database. In each case, one normal breast tissue and three different breast tumors are shown.

Complete media + doxorubicinSerum-free media + doxorubicin 0h Serum-free media

6h Serum-free media 24h Serum-free media 48h Serum-free media 72h Serum-free media 96h Serum-free media

Complete media only IP staining Complete media

2.02%

96.48%

0.09%

1.41%

84.39%

0.70%

14.83%

0.08%

84.14%

1.20%

14.60%

0.06%

0.35%

96.54%

1.65%

1.46%

0.78%

95.30%

1.97%

1.96%

0.18%

97.52%

1.31%

1.00%

0.19%

97.35%

1.57%

0.88%

1.35%

95.45%

2.48%

0.71%

IP+ IP+, A+

IP-, A-

0.39%

97.53%

1.57%

0.51%

0.33%

95.52%

2.31%

1.85%

Suppl. Figure 1

CTGF

Suppl. Figure 2

CYR61

IGFBP3

MIF

MMP2

ANP32A

APEX1

HNRNPA2B1

HNRNPK

SUB1

SNRNP70

SSB

SUMO2

THRAP3

UB2L3