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(RRJoCB)
ISSN 2349-3720
Research & Reviews
Journal of Computational Biology
www.stmjournals.comSTM JOURNALSScientific Technical Medical
Jan - April 2014
sIn silico Evaluation of α-Amylase Inhibitory Activity of Some Commercially Available Flavonoids
sIsolation of 16S rRNA and Phylogenetic Analysis of Bacillus Flexus Colonizing in Berry of Coffea
arabica
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STM JOURNALS
1. In silico Evaluation of á-Amylase Inhibitory Activity of Some Commercially Available Flavonoids Arumugam Madeswaran, Kuppusamy Asokkumar, Muthuswamy Umamaheswari, Thirumalaisamy
Sivashanmugam, Varadharajan Subhadradevi, Puliyath Jagannath 1
2. Isolation of 16S rRNA and Phylogenetic Analysis of Bacillus Flexus Colonizing in Berry of Coffea arabica Anupama R, Makari HK, Hosmani Shankar P 6
ContentsResearch & Reviews : Journal of Computational Biology
RRJoCB (2014)© STM Journals 2014. All Rights Reserved
Research & Reviews: Journal of Computational Biology ISSN: 2349-3720
Volume 3, Issue 1
www.stmjournals.com
In silico Evaluation of α-Amylase Inhibitory Activity of
Some Commercially Available Flavonoids
Arumugam Madeswaran*, Kuppusamy Asokkumar, Muthuswamy Umamaheswari,
Thirumalaisamy Sivashanmugam, Varadharajan Subhadradevi, Puliyath Jagannath Department of Pharmacology, College of Pharmacy,
Sri Ramakrishna Institute of Paramedical Sciences, Coimbatore, Tamil Nadu, India.
Abstract Diabetes has become a leading killer disease in recent years. Diabetes mellitus is a metabolic disorder characterized by hyperglycemia resulting from defects in insulin
secretion, insulin action or both. The primary objective of this study was to investigate
the α-amylase inhibitory activity of flavonoids using in silico docking studies. In this perspective, flavonoids like apigenin, baicalin, fisetin, scopoletin and wogonin were
selected. Acarbose, a known α-amylase inhibitor was used as the standard. In silico
docking studies were carried out using AutoDock 4.2, based on the Lamarckian genetic algorithm principle. The results showed that all the selected flavonoids showed binding
energy ranging between -6.84 kcal/mol to -5.36 kcal/mol when compared with that of the standard (-2.94 kcal/mol). Inhibition constant (9.71 µM to 116.96 µM) and
intermolecular energy (-8.03 kcal/mol to -5.96 kcal/mol) of the flavonoids also coincide
with the binding energy. All the selected flavonoids showed excellent α-amylase inhibitory activity when compared with the standard because of their structural
properties. The α-amylase inhibitory activity of the selected flavonoids was in order of
baicalin>apigenin>fisetin>wogonin>scopoletin. These molecular docking analyses could lead to the further development of potential α-amylase inhibitors for the treatment
of diabetes.
Keywords: Acarbose, Diabetes, Binding energy, Inhibition constant, Intermolecular
energy
RRJoCB (2014)© STM Journals 2014. All Rights Reserved
Research & Reviews: Journal of Computational Biology ISSN: 2349-3720
Volume 3, Issue 1
www.stmjournals.com
Isolation of 16S rRNA and Phylogenetic Analysis of
Bacillus Flexus Colonizing in Berry of Coffea arabica
Anupama R1,
Makari HK2, Hosmani Shankar P
3* 1PRIST University, Vallam, Thanjuvar (TN), India
2IDSG Government College, Chickmagalure-577012, India
3SBRR Mahajana First Grade College, Jayalakshmipuram, Mysore-570012, India
Abstract Bacillus flexus is a novel Gram-positive, alkaliphilic bacterium. It was isolated from
bacterial populations in heavily infected fruits of Coffea arabica. It has the ability to
synthesize active enzymes under extreme pH conditions. The 16S rDNA sequence of the organism was analyzed using BLAST program (NCBI Genbank) to verify the most closely
related data base sequences. Clustal X version 1.8 was used to determine multiple sequence matches. 16S rDNA of the bacterium was amplified by PCR using inverted
primer (Eubacteria specific primers). Genbank, USA has confirmed the sequence by
allotting accession No. KJ459005 for the 16S rRNA sequence of Bacillus flexus. This strain has been colonizing in fruits of coffee acting as a co-infect ant, hitherto not
reported.
Keywords: Bacillus flexus, Coffea arabica, 16S rDNA sequence, Genbank Ac. No
KJ459005