(RRJoCB)

ISSN 2349-3720

Research & Reviews

Journal of Computational Biology

www.stmjournals.comSTM JOURNALSScientific Technical Medical

Jan - April 2014

sIn silico Evaluation of α-Amylase Inhibitory Activity of Some Commercially Available Flavonoids

sIsolation of 16S rRNA and Phylogenetic Analysis of Bacillus Flexus Colonizing in Berry of Coffea

arabica

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STM JOURNALS

1. In silico Evaluation of á-Amylase Inhibitory Activity of Some Commercially Available Flavonoids Arumugam Madeswaran, Kuppusamy Asokkumar, Muthuswamy Umamaheswari, Thirumalaisamy

Sivashanmugam, Varadharajan Subhadradevi, Puliyath Jagannath 1

2. Isolation of 16S rRNA and Phylogenetic Analysis of Bacillus Flexus Colonizing in Berry of Coffea arabica Anupama R, Makari HK, Hosmani Shankar P 6

ContentsResearch & Reviews : Journal of Computational Biology

RRJoCB (2014)© STM Journals 2014. All Rights Reserved

Research & Reviews: Journal of Computational Biology ISSN: 2349-3720

Volume 3, Issue 1

www.stmjournals.com

In silico Evaluation of α-Amylase Inhibitory Activity of

Some Commercially Available Flavonoids

Arumugam Madeswaran*, Kuppusamy Asokkumar, Muthuswamy Umamaheswari,

Thirumalaisamy Sivashanmugam, Varadharajan Subhadradevi, Puliyath Jagannath Department of Pharmacology, College of Pharmacy,

Sri Ramakrishna Institute of Paramedical Sciences, Coimbatore, Tamil Nadu, India.

Abstract Diabetes has become a leading killer disease in recent years. Diabetes mellitus is a metabolic disorder characterized by hyperglycemia resulting from defects in insulin

secretion, insulin action or both. The primary objective of this study was to investigate

the α-amylase inhibitory activity of flavonoids using in silico docking studies. In this perspective, flavonoids like apigenin, baicalin, fisetin, scopoletin and wogonin were

selected. Acarbose, a known α-amylase inhibitor was used as the standard. In silico

docking studies were carried out using AutoDock 4.2, based on the Lamarckian genetic algorithm principle. The results showed that all the selected flavonoids showed binding

energy ranging between -6.84 kcal/mol to -5.36 kcal/mol when compared with that of the standard (-2.94 kcal/mol). Inhibition constant (9.71 µM to 116.96 µM) and

intermolecular energy (-8.03 kcal/mol to -5.96 kcal/mol) of the flavonoids also coincide

with the binding energy. All the selected flavonoids showed excellent α-amylase inhibitory activity when compared with the standard because of their structural

properties. The α-amylase inhibitory activity of the selected flavonoids was in order of

baicalin>apigenin>fisetin>wogonin>scopoletin. These molecular docking analyses could lead to the further development of potential α-amylase inhibitors for the treatment

of diabetes.

Keywords: Acarbose, Diabetes, Binding energy, Inhibition constant, Intermolecular

energy

RRJoCB (2014)© STM Journals 2014. All Rights Reserved

Research & Reviews: Journal of Computational Biology ISSN: 2349-3720

Volume 3, Issue 1

www.stmjournals.com

Isolation of 16S rRNA and Phylogenetic Analysis of

Bacillus Flexus Colonizing in Berry of Coffea arabica

Anupama R1,

Makari HK2, Hosmani Shankar P

3* 1PRIST University, Vallam, Thanjuvar (TN), India

2IDSG Government College, Chickmagalure-577012, India

3SBRR Mahajana First Grade College, Jayalakshmipuram, Mysore-570012, India

Abstract Bacillus flexus is a novel Gram-positive, alkaliphilic bacterium. It was isolated from

bacterial populations in heavily infected fruits of Coffea arabica. It has the ability to

synthesize active enzymes under extreme pH conditions. The 16S rDNA sequence of the organism was analyzed using BLAST program (NCBI Genbank) to verify the most closely

related data base sequences. Clustal X version 1.8 was used to determine multiple sequence matches. 16S rDNA of the bacterium was amplified by PCR using inverted

primer (Eubacteria specific primers). Genbank, USA has confirmed the sequence by

allotting accession No. KJ459005 for the 16S rRNA sequence of Bacillus flexus. This strain has been colonizing in fruits of coffee acting as a co-infect ant, hitherto not

reported.

Keywords: Bacillus flexus, Coffea arabica, 16S rDNA sequence, Genbank Ac. No

KJ459005