MOLPHARM/2005/012682Fig S1

FL

AG

hsp

90 [

1-30

9]

FL

AG

hsp

90 [

1-45

6]

FL

AG

hsp

90 [

1-53

0]

FL

AG

hsp

90 [

530-

724]

IP: anti-FLAG WB: anti-1

100

55.9

38.3

kDa

100

55.9

38.3

kDa

IP: anti-FLAG WB: anti-FLAG

Cell lysateWB: anti-FLAG

Cell lysateWB: anti-1

myc-1

1

1

Fig. S1. COS cells were co-transfected with the cDNAs encoding myc-tagged full-length β1 and various deletion mutants of hsp90. Immuno-precipitation (IP) was performed using a FLAG antibody and precipitates were tested for the presence of β1. Blots are representative of experiments repeated at least twice with identical results. Numbers identify the relative positions in the amino acid sequence; retained sequences are bracketed.