MOLPHARM/2005/012682Fig S1
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MOLPHARM/2005/012682Fig S1 FLAG hsp90 [1-309] FLAG hsp90 [1-456] FLAG hsp90 [1-530] FLAG hsp90 [530-724] IP: anti-FLAG WB: anti- 1 100 55.9 38.3 kDa 100 55.9 38.3 kDa IP: anti-FLAG WB: anti-FLAG Cell lysate WB: anti-FLAG Cell lysate WB: anti- 1 myc- 1 1 1 co-transfected with the cDNAs encoding myc-tagged full-length β1 and various deletion mutants of hsp performed using a FLAG antibody and precipitates were tested for the presence of β1. Blots are repres least twice with identical results. Numbers identify the relative positions in the amino acid sequen
description
myc- b 1. FLAG hsp90 [530-724]. FLAG hsp90 [1-309]. FLAG hsp90 [1-456]. FLAG hsp90 [1-530]. IP: anti-FLAG WB: anti- b 1. b 1. b 1. 100. 55.9. IP: anti-FLAG WB: anti-FLAG. 38.3. kDa. Cell lysate WB: anti- b 1. 100. 55.9. Cell lysate WB: anti-FLAG. 38.3. kDa. - PowerPoint PPT Presentation
Transcript of MOLPHARM/2005/012682Fig S1
MOLPHARM/2005/012682Fig S1
FL
AG
hsp
90 [
1-30
9]
FL
AG
hsp
90 [
1-45
6]
FL
AG
hsp
90 [
1-53
0]
FL
AG
hsp
90 [
530-
724]
IP: anti-FLAG WB: anti-1
100
55.9
38.3
kDa
100
55.9
38.3
kDa
IP: anti-FLAG WB: anti-FLAG
Cell lysateWB: anti-FLAG
Cell lysateWB: anti-1
myc-1
1
1
Fig. S1. COS cells were co-transfected with the cDNAs encoding myc-tagged full-length β1 and various deletion mutants of hsp90. Immuno-precipitation (IP) was performed using a FLAG antibody and precipitates were tested for the presence of β1. Blots are representative of experiments repeated at least twice with identical results. Numbers identify the relative positions in the amino acid sequence; retained sequences are bracketed.
