WT KO

InsulinPdx4

Figure S1

A

Lactate levels (mice)A

P< 0.009

NADPH levels (livers)B

P< 0.03

Figure S2

Expression levels of glycolytic genes in KO MEFsA

Expression levels of TCA cycle genes in KO MEFsB

Figure S3

Expression levels of Pentose Phosphate Pathway genes (MEFs)C Expression levels of Gluconeogenesis genes (MEFs)D

Expression levels of Gluconeogenesis genes (livers)E

Figure S3

A HIF1α target genes expression (MCF7) HIF1α target genes expression (MCF7)B

C

P< 0.001

P< 0.09

P< 0.001

P< 0.01

HRE-Luciferase Assay HIF1α mRNA levels in skeletal musclesD

**** **

**

**

**

* * *

Figure S4

A NBDG-glucose uptake

CB HIF1α target genes expression in KO treated miceHIF1α target genes expression in WT treated mice

**

**

****

***

Figure S5

Cont

rol

GST

WW

1

GST

WW

2

GST

WW

1+2

GST

SD

R

Cont

rol

GST

WW

1

GST

WW

2

GST

WW

1+2

GST

SD

R

MG132 HypoxiaInput

Cont

rol

GST

WW

1

GST

WW

2

GST

WW

1+2

GST

SD

R

Cont

rol

GST

WW

1

GST

WW

2

GST

WW

1+2

GST

SD

R

MG132 HypoxiaPull down

A

HIFα

GST

HIFα

GST

HSP90

WWOX interacts physically with HIF1α via its first WW domain

HIFα-OH

LAMIN

HIFα

WWOX

Anti-

ww

ox

Anti-

HIF

α

Anti-

IgG

Lysi

s

Anti-

ww

ox

Anti-

HIF

α

Anti-

IgG

Lysi

s

Normoxia (+MG 132)

Hypoxia

IP:

Endogenous WWOX physically interacts with

endogenous HIF1α

B

Figure S6