Insulin Pdx4

7
WT KO Insu lin Pdx4 Figure S1 A

description

Insulin Pdx4. A. WT. KO. Figure S1. A. Lactate levels (mice). B. NADPH levels (livers). P < 0.03. P < 0.009. Figure S2. Expression levels of glycolytic genes in KO MEFs. A. Expression levels of TCA cycle genes in KO MEFs. B. Figure S3. Expression levels of Pentose Phosphate - PowerPoint PPT Presentation

Transcript of Insulin Pdx4

Page 1: Insulin Pdx4

WT KO

InsulinPdx4

Figure S1

A

Page 2: Insulin Pdx4

Lactate levels (mice)A

P< 0.009

NADPH levels (livers)B

P< 0.03

Figure S2

Page 3: Insulin Pdx4

Expression levels of glycolytic genes in KO MEFsA

Expression levels of TCA cycle genes in KO MEFsB

Figure S3

Page 4: Insulin Pdx4

Expression levels of Pentose Phosphate Pathway genes (MEFs)C Expression levels of Gluconeogenesis genes (MEFs)D

Expression levels of Gluconeogenesis genes (livers)E

Figure S3

Page 5: Insulin Pdx4

A HIF1α target genes expression (MCF7) HIF1α target genes expression (MCF7)B

C

P< 0.001

P< 0.09

P< 0.001

P< 0.01

HRE-Luciferase Assay HIF1α mRNA levels in skeletal musclesD

**** **

**

**

**

* * *

Figure S4

Page 6: Insulin Pdx4

A NBDG-glucose uptake

CB HIF1α target genes expression in KO treated miceHIF1α target genes expression in WT treated mice

**

**

****

***

Figure S5

Page 7: Insulin Pdx4

Cont

rol

GST

WW

1

GST

WW

2

GST

WW

1+2

GST

SD

R

Cont

rol

GST

WW

1

GST

WW

2

GST

WW

1+2

GST

SD

R

MG132 HypoxiaInput

Cont

rol

GST

WW

1

GST

WW

2

GST

WW

1+2

GST

SD

R

Cont

rol

GST

WW

1

GST

WW

2

GST

WW

1+2

GST

SD

R

MG132 HypoxiaPull down

A

HIFα

GST

HIFα

GST

HSP90

WWOX interacts physically with HIF1α via its first WW domain

HIFα-OH

LAMIN

HIFα

WWOX

Anti-

ww

ox

Anti-

HIF

α

Anti-

IgG

Lysi

s

Anti-

ww

ox

Anti-

HIF

α

Anti-

IgG

Lysi

s

Normoxia (+MG 132)

Hypoxia

IP:

Endogenous WWOX physically interacts with

endogenous HIF1α

B

Figure S6