Search results for Intracellular Cytokine Staining (ICS) Flow Cytometry Assay 1 Kent J. Weinhold, Duke CFAR Director November 6th, 2013 CFAR Director’s Meeting Flow Cytometry.

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Primary Antibodies and Bioconjugates 3 Antibody Labeling Kits 4 Apoptosis and necrosis 8 Mitochondrial membrane potential dyes Flow Cytometry Accessories 10 Spectral Flow

Secondary antibodies and bioconjugates ..... p. 3 Antibody labeling kits ..... p. 4 Cellular assays ..... pp. 5-8 • Cell viability ..... p. 5 • Mitochondrial membrane

Supplementary data Supplementary Table e-1. Flow cytometry reagents and staining combinations Reagents CD3 PerCP-Cy5.5 SK7 Biolegend IL-22 PerCP- Integrin β7 FITC FIB504

Slide 1 Intracellular Cytokine Staining (ICS) Flow Cytometry Assay 1 Kent J. Weinhold, Duke CFAR Director November 6th, 2013 CFAR Director’s Meeting Flow Cytometry Workshop…

P P P P P P P P P P P P Tyr505 TCR Tyr319 Tyr171 Ras-Erk Rho/Rac pathways CD4 Lck Tyr394 Grb2 PLC 1γ Tyr142 Tyr191 Tyr783 Tyr113 Tyr128 LAT SLP76 Vav PIP2 DAGIP3 PKC Ca2+…

Polychromatic Flow Cytometry and Flow Applications Basic Concepts Kevin P. Weller Technical Applications Specialist T Cell ζ ζ TCR T Cell ζ ζ Co-stimulation MAPK promotor…

MoFlo™ Astrios* Enhanced Forward Scatter+: 02 μm to 30 μm Dynamic FSC Detection Dr Carley D Ross Mike Morrell Robin Morris Blake McCarty Alan Dean PhD and Nicole Lewis…

Table S1. Antibodies and reagents used for flow cytometry Antigen Conjugate Isotype Clone and source B220 PerCP Rat IgG2a κ RA3-6B2, BD Pharmingen TM CD19 Bv421 Rat IgG2a…

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Noh et al. Supplemental Data 1 Supplemental Methods Generation of murine ES cells with a dox-inducible Gata1 shRNA transgene. A2lox.cre ES cells (16) were maintained on irradiated…

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Morphological features of IFN-γ–stimulated mesenchymal stromal cells predict overall immunosuppressive capacity Matthew W. Klinkera,1, Ross A. Markleina,1, Jessica L.…

Glucose Metabolism and AMPK Signaling Regulate Dopaminergic Cell Death Induced by Gene α-Synuclein-Environment Paraquat Interactions Annadurai Anandhan1,2 Shulei Lei3 Roman…

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ATM ATR Perforin CTL IRE1α Caspase-10 Caspase-3 Caspase-6 Caspase-12 Caspase-9 ASK1 MKK7 PKC PI3K Akt PDK1 PIP2 PIP3 p90RSK Bad Bax Bim Bid tBid Bcl-2 Bcl-2 TRAF2 ASK1 IAP…

ENDOPLASMIC RETICULUM STRESS IN PANCREATIC β-CELLS ER STRESS AND CYTOKINE-MEDIATED β-CELL DYSFUNCTION AND APOPTOSIS ANALYSIS OF THE ER STRESS RESPONSE IN A PANCREATIC β-CELL…

Supplementary Materials and Methods Cell viability assay Cells were seeded onto a 96-well plate and cultured for 72 h Then 5 μgmL of 3-45-dimethylthiazol-2-yl-5-3-carboxymethoxyphenyl-…

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