Human Mouse

IFN-I, IFN-III (IFN-λ)CXCL-10 (IP-10)

TNF

IL-1β, IL-6, IL-8 IL-10, IL-12

IL-23TNF

Lineage–, CD45+, MHCII (HLA-DR)+, CD11c+

CD16CD64CD1a, CD1c CD11b, CD14+/–

CD172a (Sirp-α) CD206, CD209 (DC-SIGN)CD367 (DCIR/CLEC4A)CD369 (Dectin-1/CLEC7A)CD371 (CLEC12A)CLEC6ATLR3lo, TLR4, TLR7, TLR8CD207 (Langerin)–

Lineage–, CD45+,MHCII (HLA-DR)+, CD11c+

CD1a, CD64CD11bCD1cCD172a (Sirp-α)CD367 (DCIR/CLEC4A)CD369 (Dectin-1/CLEC7A)CLEC6ATLR1–3, TLR6, TLR7, TLR10CD14–

CD207 (Langerin)–

CD209 (DC-SIGN)–

CD324 (E-Cadherin)–

CD326 (EpCAM)–

Lineage–, CD45+,MHCII (HLA-DR)+, CD11c+

CD14CD209 (DC-SIGN)CD11bCD1cCD172a (Sirp-α)+ CD367 (DCIR/CLEC4A)CD369 (Dectin-1/CLEC7A)TLR1–3, TLR6, TLR7CD1a–

CD207 (Langerin)–

CD324 (E-Cadherin)–

CD326 (EpCAM)–

IL-1β, IL-6, IL-10,IL-12, IL-23, TNF

CD14

CD117 (c-kit)

CD135/FLT3CD117 (c-kit)

IRF7, IRF8,SpiB

Lineage–, CD45+,MHCII (HLA-DR)+, CD11c+

CD207 (Langerin)CD324 (E-Cadherin)CD326 (EpCAM)CD11blo

CD1ahi, CD1cCD172a (Sirp-α)CD369 (Dectin-1/CLEC7A)CD371 (CLEC12A)CLEC6A TLR1, TLR2, TLR3lo, TLR6CD14–

Lineage–, CD45+,MHCII (HLA-DR)+, CD11clo/–

CD123hi

CD303CD304 CD85g (ILT7)CD4CD45RACD272 (BTLA)CD367 (DCIR/CLEC4A)CD371 (CLEC12A)±

TLR7hi, TLR9hi

CD11b–

CD14–

IFN-I, IFN-III (IFN-λ)IL-6, IL-8

IP-10 (CXCL10)TNF

Lineage–, CD45+, MHCII (HLA-DR)+, CD11c+/lo

CD141hi

CD370 (CLEC9A)NECL2 CD11blo

CD205 (DEC-205)hi

CD272 (BTLA)hi

CD366 (TIM-3)CD367 (DCIR/CLEC4A)CD369 (Dectin-1/CLEC7A)CD371 (CLEC12A)TLR3, TLR8XCR1CD1a–, CD14–

Lineage–, CD45+,MHCII (HLA-DR)+, CD11c+

CD1cCD172a (Sirp-α)CLEC6ACD11b+/lo

CX3CR1CD272(BTLA)CD366 (TIM-3)CD367 (DCIR/CLEC4A)CD369 (CLEC7A)CD371 (CLEC12A)TLR3lo, TLR4lo, TLR8, TLR10lo

CD1a–, CD14lo/–

CSFFLT3L

M-CSF

IL-3

CommonMyeloid

ProgenitorCells

Monocyte

CD1a+

DermalDCs

CD14+

DermalDCs

InflammatoryMonocyte-

derivedDCs

LangerhansCells

PlasmacytoidDCs

IRF8, BATF3

CD141+

ConventionalMyeloid DCsIRF4

CD1c+

ConventionalMyeloid DCs

CommonDendriticPrecursor

Cells

IFN-I, IFN-III (IFN-λ)IL-12

CXCL-10 (IP-10)TNF

IL-1β, IL-6, IL-8IL-10, IL-12

IL-23TNF

IL-1β, IL-6, IL-8IL-10, IL-12

IL-23TNF

Lineage–, CD45+,MHCII+, CD11c+

CD64 CD11bCD209a (DC-SIGN) CD272 (BTLA)lo

Ly-6CTLR1-6, TLR7-8, TLR10CD8a–, CD14–

Lineage–, CD45+,MHCII+, CD11c+

CD207 (Langerin)CD11blo

CD172a (Sirp-α)–

Lineage–, CD45+,MHCII+, CD11c+

CD11b±

CD172a (Sirp-α)CD207 (Langerin)–

Lineage–, CD45+,MHCII+, CD11c+

CD11bCD172a (Sirp-α)CD209a (DC-SIGN)±

CD272 (BTLA)lo

F4/80TLR5, TLR7, TLR9CX3CR1CD4–, CD8a–

CD14–

XCR1–

IL-1β, IL-6 IL-10,IL-12, IL-23, TNF

CD11b, CD115Ly6C

CD117 (c-kit)

CD117 (c-kit)CD135/FLT3

IRF7, IRF8, BATF3hiSpiB, Zbtb46–

Lineage–, CD45+,MHCII+, CD11c+

CD207 (Langerin)CD324 (E-Cadherin)CD326 (EpCAM)CD11bCD24CD172a (Sirp-α)CD205 (DEC-205)F4/80TLR3, TLR11CD8–, CD14–

CD370 (Clec9a)–

Lineage–, CD45+,MHCII+, CD11clo

CD45R (B220)CD317 (BST-2)Ly6CSiglec-HCD4CD172a (Sirp-α)CD209a (DC-SIGN)CD272 (BTLA) CD370 (Clec9a)TLR7hi, TLR9hi

CD11b–

CD14–

Lineage–, CD45+,MHCII+, CD11chi

CD103CD205 (DEC-205)CD207 (Langerin)CD272 (BTLA)hi

CD370 (Clec9a)NECL2TLR3, TLR4, TLR11XCR1CD4–, CD8a–

CD11b–, CD14–

F4/80–

CX3CR1–

IFN-I, IFN-III (IFN-λ)IL-6, IL-8

IP-10 (CXCL10)TNF

IFN-I, IFN-III (IFN-λ)IL-12

CXCL-10 (IP-10)TNF

IL-15

Lineage–, CD45+,MHCII+, CD11chi

CD8aCD205 (DEC-205)CD207 (Langerin)+/–

CD272 (BTLA)hi

CD370 (Clec9a) NECL2TLR3, TLR4, TLR11XCR1CD4–

CD11b–

CD14–

Lineage–, CD45+,MHCII+, CD11chi

CD4CD11bCD172a (Sirp-α)CD205 (DEC-205)CD272 (BTLA)lo

ESAMF4/80TLR5, TLR7, TLR9 CX3CR1CD8a–

CD14–

CSFFLT3L

TGFb1M-CSF

FLT3LIFN Type I

FLT3L

CommonMyeloid

ProgenitorCells

Monocyte

CD207+

DermalDCs

CD207–Dermal

DCs

InflammatoryMonocyte-

derivedDCs

LangerhansCells

PlasmacytoidDCs

IRF8, BATF3,Zbtb46

CD8a+

ConventionalLymphoid

Resident DCsIRF4, Zbtb46

BATF3hi

CD4+Conventional

LymphoidResident DCs

IRF4, IRF2Zbtb46, BATF3hi

CD11b+

ConventionalMigratory

DCs

CommonDendriticPrecursor

Cells

Pre-Conventional

DendriticCell

IRF8, BATF3,Zbtb46

CD103+

ConventionalMigratory

DCs

Zbtb46–

IL-15

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Overview of Dendritic Cell Development

Core dendritic cell phenotyping markers Lineage (Hu): CD3–, CD19–, CD20–, CD56–, CD141

Lineage (Ms): CD3–, CD19–, CD49b– or NK1.1–, CD14–

Dendritic cell subset markers (positive) TLR7, TLR8 and TLR9 are endosomal and require

intracellular staining

Dendritic cell subset markers (negative)

Secreted cytokinesTranscription factorsMarkers on this schematic can be altered as a result of cellular environment, differentiation state and other factors. DC biomarker profiles were obtained from RNA and protein level analysis provided in peer reviewed publications and public databases. Visit bdbiosciences.com/DC for more information.

KEY

1 CD14 is negative or low in all Human DC subsets except for CD14+ Dermal DCs and inflammatory DCs.