Overview of Dendritic Cell Development Poster
1
Human Mouse IFN-I, IFN-III (IFN-λ) CXCL-10 (IP-10) TNF IL-1β, IL-6, IL-8 IL-10, IL-12 IL-23 TNF Lineage – , CD45 + , MHCII (HLA-DR) + , CD11c + CD16 CD64 CD1a, CD1c CD11b, CD14 +/– CD172a (Sirp-α) CD206, CD209 (DC-SIGN) CD367 (DCIR/CLEC4A) CD369 (Dectin-1/CLEC7A) CD371 (CLEC12A) CLEC6A TLR3 lo , TLR4, TLR7, TLR8 CD207 (Langerin) – Lineage – , CD45 + , MHCII (HLA-DR) + , CD11c + CD1a, CD64 CD11b CD1c CD172a (Sirp-α) CD367 (DCIR/CLEC4A) CD369 (Dectin-1/CLEC7A) CLEC6A TLR1–3, TLR6, TLR7, TLR10 CD14 – CD207 (Langerin) – CD209 (DC-SIGN) – CD324 (E-Cadherin) – CD326 (EpCAM) – Lineage – , CD45 + , MHCII (HLA-DR) + , CD11c + CD14 CD209 (DC-SIGN) CD11b CD1c CD172a (Sirp-α) + CD367 (DCIR/CLEC4A) CD369 (Dectin-1/CLEC7A) TLR1–3, TLR6, TLR7 CD1a – CD207 (Langerin) – CD324 (E-Cadherin) – CD326 (EpCAM) – IL-1β, IL-6, IL-10, IL-12, IL-23, TNF CD14 CD117 (c-kit) CD135/FLT3 CD117 (c-kit) IRF7, IRF8, SpiB Lineage – , CD45 + , MHCII (HLA-DR) + , CD11c + CD207 (Langerin) CD324 (E-Cadherin) CD326 (EpCAM) CD11b lo CD1a hi , CD1c CD172a (Sirp-α) CD369 (Dectin-1/CLEC7A) CD371 (CLEC12A) CLEC6A TLR1, TLR2, TLR3 lo , TLR6 CD14 – Lineage – , CD45 + , MHCII (HLA-DR) + , CD11c lo/– CD123 hi CD303 CD304 CD85g (ILT7) CD4 CD45RA CD272 (BTLA) CD367 (DCIR/CLEC4A) CD371 (CLEC12A) ± TLR7 hi , TLR9 hi CD11b – CD14 – IFN-I, IFN-III (IFN-λ) IL-6, IL-8 IP-10 (CXCL10) TNF Lineage – , CD45 + , MHCII (HLA-DR) + , CD11c +/lo CD141 hi CD370 (CLEC9A) NECL2 CD11b lo CD205 (DEC-205) hi CD272 (BTLA) hi CD366 (TIM-3) CD367 (DCIR/CLEC4A) CD369 (Dectin-1/CLEC7A) CD371 (CLEC12A) TLR3, TLR8 XCR1 CD1a – , CD14 – Lineage – , CD45 + , MHCII (HLA-DR) + , CD11c + CD1c CD172a (Sirp-α) CLEC6A CD11b +/lo CX3CR1 CD272(BTLA) CD366 (TIM-3) CD367 (DCIR/CLEC4A) CD369 (CLEC7A) CD371 (CLEC12A) TLR3 lo , TLR4 lo , TLR8, TLR10 lo CD1a – , CD14 lo/– CSF FLT3L M-CSF IL-3 Common Myeloid Progenitor Cells Monocyte CD1a + Dermal DCs CD14 + Dermal DCs Inflammatory Monocyte- derived DCs Langerhans Cells Plasmacytoid DCs IRF8, BATF3 CD141 + Conventional Myeloid DCs IRF4 CD1c + Conventional Myeloid DCs Common Dendritic Precursor Cells IFN-I, IFN-III (IFN-λ) IL-12 CXCL-10 (IP-10) TNF IL-1β, IL-6, IL-8 IL-10, IL-12 IL-23 TNF IL-1β, IL-6, IL-8 IL-10, IL-12 IL-23 TNF Lineage – , CD45 + , MHCII + , CD11c + CD64 CD11b CD209a (DC-SIGN) CD272 (BTLA) lo Ly-6C TLR1-6, TLR7-8, TLR10 CD8a – , CD14 – Lineage – , CD45 + , MHCII + , CD11c + CD207 (Langerin) CD11b lo CD172a (Sirp-α) – Lineage – , CD45 + , MHCII + , CD11c + CD11b ± CD172a (Sirp-α) CD207 (Langerin) – Lineage – , CD45 + , MHCII + , CD11c + CD11b CD172a (Sirp-α) CD209a (DC-SIGN) ± CD272 (BTLA) lo F4/80 TLR5, TLR7, TLR9 CX3CR1 CD4 – , CD8a – CD14 – XCR1 – IL-1β, IL-6 IL-10, IL-12, IL-23, TNF CD11b, CD115 Ly6C CD117 (c-kit) CD117 (c-kit) CD135/FLT3 IRF7, IRF8, BATF3hi SpiB, Zbtb46 – Lineage – , CD45 + , MHCII + , CD11c + CD207 (Langerin) CD324 (E-Cadherin) CD326 (EpCAM) CD11b CD24 CD172a (Sirp-α) CD205 (DEC-205) F4/80 TLR3, TLR11 CD8 – , CD14 – CD370 (Clec9a) – Lineage – , CD45 + , MHCII + , CD11c lo CD45R (B220) CD317 (BST-2) Ly6C Siglec-H CD4 CD172a (Sirp-α) CD209a (DC-SIGN) CD272 (BTLA) CD370 (Clec9a) TLR7 hi , TLR9 hi CD11b – CD14 – Lineage – , CD45 + , MHCII + , CD11c hi CD103 CD205 (DEC-205) CD207 (Langerin) CD272 (BTLA) hi CD370 (Clec9a) NECL2 TLR3, TLR4, TLR11 XCR1 CD4 – , CD8a – CD11b – , CD14 – F4/80 – CX3CR1 – IFN-I, IFN-III (IFN-λ) IL-6, IL-8 IP-10 (CXCL10) TNF IFN-I, IFN-III (IFN-λ) IL-12 CXCL-10 (IP-10) TNF IL-15 Lineage – , CD45 + , MHCII + , CD11c hi CD8a CD205 (DEC-205) CD207 (Langerin) +/– CD272 (BTLA) hi CD370 (Clec9a) NECL2 TLR3, TLR4, TLR11 XCR1 CD4 – CD11b – CD14 – Lineage – , CD45 + , MHCII + , CD11c hi CD4 CD11b CD172a (Sirp-α) CD205 (DEC-205) CD272 (BTLA) lo ESAM F4/80 TLR5, TLR7, TLR9 CX3CR1 CD8a – CD14 – CSF FLT3L TGFb1 M-CSF FLT3L IFN Type I FLT3L Common Myeloid Progenitor Cells Monocyte CD207 + Dermal DCs CD207– Dermal DCs Inflammatory Monocyte- derived DCs Langerhans Cells Plasmacytoid DCs IRF8, BATF3, Zbtb46 CD8a + Conventional Lymphoid Resident DCs IRF4, Zbtb46 BATF3 hi CD4+ Conventional Lymphoid Resident DCs IRF4, IRF2 Zbtb46, BATF3 hi CD11b + Conventional Migratory DCs Common Dendritic Precursor Cells Pre- Conventional Dendritic Cell IRF8, BATF3, Zbtb46 CD103 + Conventional Migratory DCs Zbtb46– IL-15 bdbiosciences.com © 2016 BD. BD and the BD Logo are trademarks of Becton, Dickinson and Company. BD Life Sciences, San Jose, CA, 95131, USA For Research Use Only. Not for use in diagnostic or therapeutic procedures. 23-19088-00 Overview of Dendritic Cell Development Core dendritic cell phenotyping markers Lineage (Hu): CD3 – , CD19 – , CD20 – , CD56 – , CD14 1 Lineage (Ms): CD3 – , CD19 – , CD49b – or NK1.1 – , CD14 – Dendritic cell subset markers (positive) TLR7, TLR8 and TLR9 are endosomal and require intracellular staining Dendritic cell subset markers (negative) Secreted cytokines Transcription factors Markers on this schematic can be altered as a result of cellular environment, differentiation state and other factors. DC biomarker profiles were obtained from RNA and protein level analysis provided in peer reviewed publications and public databases. Visit bdbiosciences.com/DC for more information. KEY 1 CD14 is negative or low in all Human DC subsets except for CD14 + Dermal DCs and inflammatory DCs.
Transcript of Overview of Dendritic Cell Development Poster
Human Mouse
IFN-I, IFN-III (IFN-λ)CXCL-10 (IP-10)
TNF
IL-1β, IL-6, IL-8 IL-10, IL-12
IL-23TNF
Lineage–, CD45+, MHCII (HLA-DR)+, CD11c+
CD16CD64CD1a, CD1c CD11b, CD14+/–
CD172a (Sirp-α) CD206, CD209 (DC-SIGN)CD367 (DCIR/CLEC4A)CD369 (Dectin-1/CLEC7A)CD371 (CLEC12A)CLEC6ATLR3lo, TLR4, TLR7, TLR8CD207 (Langerin)–
Lineage–, CD45+,MHCII (HLA-DR)+, CD11c+
CD1a, CD64CD11bCD1cCD172a (Sirp-α)CD367 (DCIR/CLEC4A)CD369 (Dectin-1/CLEC7A)CLEC6ATLR1–3, TLR6, TLR7, TLR10CD14–
CD207 (Langerin)–
CD209 (DC-SIGN)–
CD324 (E-Cadherin)–
CD326 (EpCAM)–
Lineage–, CD45+,MHCII (HLA-DR)+, CD11c+
CD14CD209 (DC-SIGN)CD11bCD1cCD172a (Sirp-α)+ CD367 (DCIR/CLEC4A)CD369 (Dectin-1/CLEC7A)TLR1–3, TLR6, TLR7CD1a–
CD207 (Langerin)–
CD324 (E-Cadherin)–
CD326 (EpCAM)–
IL-1β, IL-6, IL-10,IL-12, IL-23, TNF
CD14
CD117 (c-kit)
CD135/FLT3CD117 (c-kit)
IRF7, IRF8,SpiB
Lineage–, CD45+,MHCII (HLA-DR)+, CD11c+
CD207 (Langerin)CD324 (E-Cadherin)CD326 (EpCAM)CD11blo
CD1ahi, CD1cCD172a (Sirp-α)CD369 (Dectin-1/CLEC7A)CD371 (CLEC12A)CLEC6A TLR1, TLR2, TLR3lo, TLR6CD14–
Lineage–, CD45+,MHCII (HLA-DR)+, CD11clo/–
CD123hi
CD303CD304 CD85g (ILT7)CD4CD45RACD272 (BTLA)CD367 (DCIR/CLEC4A)CD371 (CLEC12A)±
TLR7hi, TLR9hi
CD11b–
CD14–
IFN-I, IFN-III (IFN-λ)IL-6, IL-8
IP-10 (CXCL10)TNF
Lineage–, CD45+, MHCII (HLA-DR)+, CD11c+/lo
CD141hi
CD370 (CLEC9A)NECL2 CD11blo
CD205 (DEC-205)hi
CD272 (BTLA)hi
CD366 (TIM-3)CD367 (DCIR/CLEC4A)CD369 (Dectin-1/CLEC7A)CD371 (CLEC12A)TLR3, TLR8XCR1CD1a–, CD14–
Lineage–, CD45+,MHCII (HLA-DR)+, CD11c+
CD1cCD172a (Sirp-α)CLEC6ACD11b+/lo
CX3CR1CD272(BTLA)CD366 (TIM-3)CD367 (DCIR/CLEC4A)CD369 (CLEC7A)CD371 (CLEC12A)TLR3lo, TLR4lo, TLR8, TLR10lo
CD1a–, CD14lo/–
CSFFLT3L
M-CSF
IL-3
CommonMyeloid
ProgenitorCells
Monocyte
CD1a+
DermalDCs
CD14+
DermalDCs
InflammatoryMonocyte-
derivedDCs
LangerhansCells
PlasmacytoidDCs
IRF8, BATF3
CD141+
ConventionalMyeloid DCsIRF4
CD1c+
ConventionalMyeloid DCs
CommonDendriticPrecursor
Cells
IFN-I, IFN-III (IFN-λ)IL-12
CXCL-10 (IP-10)TNF
IL-1β, IL-6, IL-8IL-10, IL-12
IL-23TNF
IL-1β, IL-6, IL-8IL-10, IL-12
IL-23TNF
Lineage–, CD45+,MHCII+, CD11c+
CD64 CD11bCD209a (DC-SIGN) CD272 (BTLA)lo
Ly-6CTLR1-6, TLR7-8, TLR10CD8a–, CD14–
Lineage–, CD45+,MHCII+, CD11c+
CD207 (Langerin)CD11blo
CD172a (Sirp-α)–
Lineage–, CD45+,MHCII+, CD11c+
CD11b±
CD172a (Sirp-α)CD207 (Langerin)–
Lineage–, CD45+,MHCII+, CD11c+
CD11bCD172a (Sirp-α)CD209a (DC-SIGN)±
CD272 (BTLA)lo
F4/80TLR5, TLR7, TLR9CX3CR1CD4–, CD8a–
CD14–
XCR1–
IL-1β, IL-6 IL-10,IL-12, IL-23, TNF
CD11b, CD115Ly6C
CD117 (c-kit)
CD117 (c-kit)CD135/FLT3
IRF7, IRF8, BATF3hiSpiB, Zbtb46–
Lineage–, CD45+,MHCII+, CD11c+
CD207 (Langerin)CD324 (E-Cadherin)CD326 (EpCAM)CD11bCD24CD172a (Sirp-α)CD205 (DEC-205)F4/80TLR3, TLR11CD8–, CD14–
CD370 (Clec9a)–
Lineage–, CD45+,MHCII+, CD11clo
CD45R (B220)CD317 (BST-2)Ly6CSiglec-HCD4CD172a (Sirp-α)CD209a (DC-SIGN)CD272 (BTLA) CD370 (Clec9a)TLR7hi, TLR9hi
CD11b–
CD14–
Lineage–, CD45+,MHCII+, CD11chi
CD103CD205 (DEC-205)CD207 (Langerin)CD272 (BTLA)hi
CD370 (Clec9a)NECL2TLR3, TLR4, TLR11XCR1CD4–, CD8a–
CD11b–, CD14–
F4/80–
CX3CR1–
IFN-I, IFN-III (IFN-λ)IL-6, IL-8
IP-10 (CXCL10)TNF
IFN-I, IFN-III (IFN-λ)IL-12
CXCL-10 (IP-10)TNF
IL-15
Lineage–, CD45+,MHCII+, CD11chi
CD8aCD205 (DEC-205)CD207 (Langerin)+/–
CD272 (BTLA)hi
CD370 (Clec9a) NECL2TLR3, TLR4, TLR11XCR1CD4–
CD11b–
CD14–
Lineage–, CD45+,MHCII+, CD11chi
CD4CD11bCD172a (Sirp-α)CD205 (DEC-205)CD272 (BTLA)lo
ESAMF4/80TLR5, TLR7, TLR9 CX3CR1CD8a–
CD14–
CSFFLT3L
TGFb1M-CSF
FLT3LIFN Type I
FLT3L
CommonMyeloid
ProgenitorCells
Monocyte
CD207+
DermalDCs
CD207–Dermal
DCs
InflammatoryMonocyte-
derivedDCs
LangerhansCells
PlasmacytoidDCs
IRF8, BATF3,Zbtb46
CD8a+
ConventionalLymphoid
Resident DCsIRF4, Zbtb46
BATF3hi
CD4+Conventional
LymphoidResident DCs
IRF4, IRF2Zbtb46, BATF3hi
CD11b+
ConventionalMigratory
DCs
CommonDendriticPrecursor
Cells
Pre-Conventional
DendriticCell
IRF8, BATF3,Zbtb46
CD103+
ConventionalMigratory
DCs
Zbtb46–
IL-15
bdbiosciences.com© 2016 BD. BD and the BD Logo are trademarks of Becton, Dickinson and Company.
BD Life Sciences, San Jose, CA, 95131, USA
For Research Use Only. Not for use in diagnostic or therapeutic procedures.23-19088-00
Overview of Dendritic Cell Development
Core dendritic cell phenotyping markers Lineage (Hu): CD3–, CD19–, CD20–, CD56–, CD141
Lineage (Ms): CD3–, CD19–, CD49b– or NK1.1–, CD14–
Dendritic cell subset markers (positive) TLR7, TLR8 and TLR9 are endosomal and require
intracellular staining
Dendritic cell subset markers (negative)
Secreted cytokinesTranscription factorsMarkers on this schematic can be altered as a result of cellular environment, differentiation state and other factors. DC biomarker profiles were obtained from RNA and protein level analysis provided in peer reviewed publications and public databases. Visit bdbiosciences.com/DC for more information.
KEY
1 CD14 is negative or low in all Human DC subsets except for CD14+ Dermal DCs and inflammatory DCs.
