MERGE DAPI α-Kac MERGE DAPIα-Kcrα-HA α-HA
D1
09
F-V
RP
P
D1
09
F-V
RP
P
D1
09
F
D1
09
F
H1
41
AS
42
1A
S4
21
D
H1
41
AS
42
1A
S4
21
D
HA
-HD
AC
1
HA
-HD
AC
1
A
E4
26
AE
42
4A
E4
26
AE
42
4A
B
Supplementary Figure S7. Various HDAC1 mutations result in loss of both HDAC andHDCR activities. (A) and (B) The various HDAC1 mutants were examined for HDCR activity(A) or HDAC activity (B) by ectopic expression in HeLa cells followed by immunostainingusing pan-Kcr or pan-Kac antibody as indicated. Scale bar:15 μm.
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