varian instruments
1
1. IMPURITY 2. PHENETOLE 3. NITROBENZENE 4. METHYL BENZOATE 5. CARBAZOLE 6. ACÉTOPHENONE 7. Z, 4- DlNITROBENZCNC Instrument: Varian Aerograph 4100 Liquid Chromatograph Column: MicroPak SI-10 Length: 15 cm Diameter: 2.1 mm, i.d. Mobile Phase: hexanes Sample Size: 4 μι Flow Rate: 400 ml/hrj Pressure 1400 psi Sample Concentration: Approx. 1 mg/ml of each in hexane Oetector: UV NEW-MicroPak™columns for high performance liquid chromatography A quantum advance in speed, capacity, resolution Varian Aerograph's new MicroPak columns make high performance liquid-solid chroma- tography a reality. Packed with small particle size (about 10 μτη) porous support materials, they provide higher sample capacity and chromatographic efficiency than any other LC columns available. MicroPak columns overcome the problem of slow sample diffusion into and out of the deep pores in large particle size (>40 /an) porous adsorbents. The best way to shorten the dif- fusion path is to decrease the pore depth by decreasing the adsorbent particle diameter. Theory said it could be done. MicroPak turns theory into a powerful new separating medium for liquid chromatography. These new columns offer many advantages. Sample sizes of 1 m g / g of adsorbent can be injected without loss of column efficiency. The silica particles used to pack the columns are 2700 MITCHELL DR / WALNUT CREEK / CALIFORNIA / USA 94598 D 6358 VISCOUNT RD / MALTON / ONTARIO / CANADA STEINHAUSERSTRASSE / 6300 / ZUG / SWITZERLAND Π 679 SPRINGVALE RD / N. SPRINGVALE / VICTORIA / AUSTRALIA the same particles used in TLC, only smaller. Thus, you can extrapolate directly from TLC to high performance liquid chromatography, with the added benefits of easier quantitation, greater speed and efficiency, and convenient sample collection. Flow programming can be used without the large decrease in efficiency at high flow rates often observed for larger particle sizes. Samples having widely varying distribution coefficients can be separated using gradient elution. To get the full benefit of their high efficiency, MicroPak columns should be used on high per- formance liquid chromatographs with a mini- mum volume between the column outlet and the detector. Varian Aerograph's LC-4000 and LC-4100 instruments are ideal. Brochures describing both the columns and the instru- ments are yours for the asking. varian instruments CIRCLE 215 ON READER SERVICE CARD 26 A · ANALYTICAL CHEMISTRY, VOL. 44, NO. 8, JULY 1972
Transcript of varian instruments
1. IMPURITY 2. PHENETOLE 3. NITROBENZENE 4. METHYL BENZOATE 5. CARBAZOLE 6. ACÉTOPHENONE 7. Z, 4- DlNITROBENZCNC
Instrument: Varian Aerograph 4100 L iqu id Chromatograph
Column: MicroPak SI-10 Length: 15 cm Diameter: 2.1 m m , i.d. Mobile Phase: hexanes Sample Size: 4 μ ι F low Rate: 400 ml/hr j Pressure 1400 psi Sample Concentrat ion: Approx . 1 mg/ml
of each in hexane Oetector: UV
NEW-MicroPak™columns for high performance liquid chromatography A quantum advance in speed, capacity, resolution
Varian Aerograph's new MicroPak columns make high performance liquid-solid chromatography a reality. Packed with small particle size (about 10 μτη) porous support materials, they provide higher sample capaci ty and chromatographic efficiency than any other LC columns available. MicroPak columns overcome the problem of slow sample diffusion into and out of the deep pores in large particle size ( > 4 0 /an) porous adsorbents. The best way to shorten the diffusion path is to decrease the pore depth by decreasing the adsorbent particle diameter. Theory said it could be done. MicroPak turns theory into a powerful new separating medium for liquid chromatography. These new columns offer many advantages. Sample sizes of 1 mg/g of adsorbent can be injected without loss of column efficiency. The silica particles used to pack the columns are
2700 MITCHELL DR / WALNUT CREEK / CALIFORNIA / USA 94598 D 6358 VISCOUNT RD / MALTON / ONTARIO / CANADA STEINHAUSERSTRASSE / 6300 / ZUG / SWITZERLAND Π 679 SPRINGVALE RD / N. SPRINGVALE / VICTORIA / AUSTRALIA
the same particles used in TLC, only smaller. Thus, you can extrapolate directly from TLC to high performance liquid chromatography, with the added benefits of easier quantitation, greater speed and efficiency, and convenient sample collection. Flow programming can be used without the large decrease in efficiency at high flow rates often observed for larger particle sizes. Samples having widely varying distribution coefficients can be separated using gradient elution. To get the full benefit of their high efficiency, MicroPak columns should be used on high performance liquid chromatographs with a minimum volume between the column outlet and the detector. Varian Aerograph's LC-4000 and LC-4100 instruments are ideal. Brochures describing both the columns and the instruments are yours for the asking.
varian instruments
CIRCLE 2 1 5 O N READER SERVICE CARD
26 A · ANALYTICAL CHEMISTRY, VOL. 44, NO. 8, JULY 1972
