Tweez 200si System

Aresis Advanced Research Instrumentation Solutions

1. Technical leadership

Key technical parameters

Resolution and accuracy

Uniformity (of intensity / trap stiffness)

Linearity (of AOD response)

System stability

Speed (ghost trap elimination)

Resolution and accuracy - definition

Δ

Resolution: Accuracy:

1 2

δ = δ(t)

1 1

how precisely we can regulate relative distance between two traps (1 and 2)

how precisely we can hold the trap in one position

Resolution – Tweez 200si performance

Relative distance between two traps of which one is sine modulated with amplitude 100 nm and frequency 1Hz.

Power spectrum of mean square displacement between the two traps.

Trap positioning resolution in Tweez 200si is < 0.001 nm - well below the detection limit of particle’s position resolution.

Accuracy – managing thermal drift

Positions of trap 1 (at X1) and trap 2 (at X2) are slowly drifting (~4 nm/min).

Relative distance of trap 1 (at X1) and trap 2 (at X2) remains stable with time (~0.05 nm/min).

Uniformity (of intensity / trap stiffness)

Deviation of the AOD response reduced from ~30% to below 1%.

How uniform is the trap stiffness over the working field?

Linearity (of AOD response)

Deviation from linearity of the AOD response is reduced to below 1%.

How linear is the trap stiffness with respect to AOD RF driving power?

Stability and speed

1. Managing positional noise

Tweez 200si technological solution

High stability RF generator High-stability time base (10-6 ppm)

Mechanical stability

-Thermal drift

-Acoustic noise

Optical design (short optical path and geometry of the set-up)

Mechanical design (compact, single block machined aluminum construction)

Pointing stability of laser Fiber laser

2. Managing intensity noise Tweez 200si technological solution

Amplitude stability of laser Active laser power stabilization (<0,1%)

3. Managing speed Tweez 200si technological solution

High trap-to-trap switching frequency

Up to 100 kHz via digital signal processing (DSP) and field programmable gate array (FPGA) hardware + prevention of ghosting

Ghost-trap elimination

Tweez 200si system

Ghost traps are reduced from relative intensity of 25% to below 0.1%.

How effectively are the ghost traps eliminated at high trap-to-trap switching rates?

2. Integrated design

Integrated design of Tweez 200si system

Tweez 200si Control Unit (TCU)

Tweez 200si IR Laser Unit (TILU)

Tweez 200si Optical Unit (TOU)

Tweez 200si mounted on Nikon Eclipse TE 2000 with TIRF module installed.

Tweez 200si TCU and TILU

TCU

TILU

Size of both units mounted on each other: 430 mm x 300 mm

TCU connected to PC via USB

TCU connected to TOU via RF and control cables

TILU connected to TOU via optical fiber

Tweez 200si TOU

Features of TOU Tweez 200si Benefits

Optical module made from a solid block of a high quality machined aluminium

Stability and low mechanical noise

Completely closed system No cleaning of optics required No (re)alignment of optics required

Compatibility with other microscope add-ons

Enhancing versatility of applications

Tweez 200si mounts directly on a microscope body

Slika Stran 2 v prospektu levo spoda,

Lahko še slika, ki pokaže, kako je TWEEZ

povezan z Nikonom

Key benefits:

Complete turn-key system

Compatible with Nikon microscope add-ons (TIRF, confocal, etc.)

Ports to other Nikon modules remain open

Intuitive and powerful Tweez 200si software

Features of Tweez 200si software Tweez 200si benefits

Easy-to-use interface Effective control over trapping patterns- easy manual manipulation of optical traps- easy automatization

Open architecture Full control by a third partyTweez 200si can be controlled via TCP by user-written control software => tailoring to demands of a particular experiment

Tweez 200si advanced manipulation features

extended trapping patterns in the form of:

point traps – single diffraction limited laser spot,

complex traps – user defined pattern of laser spots.

trap automation in the form of:

manual traps – moved and adjusted manually (mouse control),

automated traps – trap is moved (rotated/scaled if it is a complex trap) automatically on a user defined path.

Tweez 200si control window example

Screen shot shows three traps. Trap 1 is a complex trap (green square pattern) periodically moved, scaled and rotated on a circular path. Traps 2 and 3 are manual traps moved with mouse cursor.

4 Examples of software use (movie)

3. Customer support

Customer support

- Technical consulting (choice of microscope components, e.g. objective etc.)

- Training- E-mail hotline (info@aresis.com)

- Remote software and firmware upgrades- 1 year limited warranty on materials and craftsmanship- Fast service times