Complies with KB of February, 8 , 1999 (BS 21.04.99) and MB of February, 12, 1999(BS 21.04.99) concerning trade and use of substances destined for animal feeding.

The only EU registered β-mannanase

FOR MORE INFORMATION:VITAMEX N.V. ● Booiebos 5 ● B-9031 DRONGEN - BELGIUMTel.: +32 (0) 9 280 29 71 ● Fax: +32 (0) 9 282 34 27 E-mail: info@vitamex.com ● www.vitamex.com

Hemicell® is a patented fermentation product of Bacillus lentus. The key ingredient in Hemicell® is an endo-1,4-ß-mannanase, which degrades high molecular weight soluble ß-galactomannan fibers (also called β-mannan) into mannose oligosaccharides (MOS).

INTRODUCTION

bIOlOgICAl EFFECTS

Hemicell® improves the performance of monogastric animals by efficiently degrading soluble β-galacto-mannan soluble fibers into smaller mannose oligosaccharides (MOS) that can’t provoke an immune response.

pRODUCT SpECIFICATIONS

1. FORMUlATION Hemicell® -D: ß-mannanase, 390 million units/kg, guaranteed minimumHemicell® -L: dark-brown solution; ß-mannanase, 720 million units/liter, guaranteed minimum

2. DOSAgE

Hemicell® -D: 200 g/metric ton Not thermo stable, for use in mash feed only.

Hemicell® -L: 110 ml/metric tonNot thermo stable. Apply to the cool pelleted feed witha suitable spray system. Dilute with water so 1.0 liter issprayed per metric ton.

3. pACKAgINg

25 kg bags Hemicell® -D208 l drums Hemicell® -L

4. STORAgE

Store Hemicell products dry and cool. Avoid prolonged exposure to temperatures above 25°C.

Hemicell® feed enzyme is the registered trademark of ChemGen Corp. Hemicell® and its use are protected by U.S. Patents 5,429,828;5,476,775 Europe Patent: EP 0531 437China Patent: ZL96107126.5 MADE IN AMERICA

EU registration number: 4a3

the only enzyme for β-mannan degradation

the only enzyme for β-mannan degradation

Hemicell® Lml / metric ton

110 Broilers

Hemicell® Dg / metric ton

200

Complies with KB of February, 8 , 1999 (BS 21.04.99) and MB of February, 12, 1999 (BS 21.04.99) concerning trade and use of substances destined for animal feeding.

Hemicell degradesß-Mannan in feed

Reduced superfluous immune provocation

Improved Animal Performance

Fewer nutrients wasted on immune reactionsLess demand on the immune system

Anabolic activity gains priority over catabolic activity

Higher production of digestive enzymesBetter feed digestibility and nutrient utilization

Better health

More net energy in feed

Hemicell® degrades ß-mannan in feed and, thus, reverses its negative impact on animal performance. Hemicell® is most effective in diets that are rich in soybean meal, sunflower meal, rapeseed meal, sesame meal, palm kernel meal, copra meal and guar meal.

All vegetable feed ingredients contain β-mannan fibers as part of the hemicellulose fraction, and soy-bean meal is the main source of β-mannan in most feeds for monogastric animals. Soluble β-mannan, or β-galactomannan, is a potent anti-nutritional factor, able to provoke a futile response of the innate immune system. During the past decade it has been documented that the relatively low levels of 0.2-0.4% β-galactomannan, found in most swine and poultry feeds, can result in significant performance losses.

ß-mannan has been shown to:

• Waste nutrients by provoking a production of cytokines and acute phase proteins. • Reduce the level of IGF-1 (insulin-like growth factor 1). • Interfere with insulin secretion.• Reduce the efficiency of nutrient deposition for growth / production.• Reduce the rate of glucose absorption.• Reduced water absorption, resulting in higher fecal moisture levels.

1. Hemicell® IMpROVES NUTRIENT UTIlIZATION

Energy by 80-150 Kcal ME/kg feed;Improves the amino acid digestibility by 1.5-2.3 %

••

The matrix values for Hemicell® were developed from digestibility experiments and confirmed by animal growth trials. Using them to formulate Hemicell® into the diets offers the best opportunity to reduce feed cost without loss of per-formance.. Using Hemicell® as an ingredient provides the opportunity to save nutrients (energy and amino acids). Results from pen trials and field trials demonstrate the nutritional benefits. The typical trial results are shown in figure 1 in broilers. Tens of millions of commercial poultry have been successfully produced in US, Latin America and Asia following this practice.

2. UNIFORMITY Hemicell® improves body weight uniformity

Broilers - 15-17 %Ducks - 12 %Turkeys - 25-40 %

•••

broilers 1broilers 2broilers 3Peking Ducks 4

Age(days)

21495442

Control

11.413.411.614.1

Hemicell®

9.9311.39.2

12.3

Improvement %

12,915.720.812.9

3. HEAlTH

Studies have shown that the benefits from Hemicell® increase significantly when the ani-mals are subject to challenges from enteric diseases including Necrotic enteritis and coccidiosis. The magnitude of the effect depends on both the ß-mannan content in the feed and the severity of the disease challenge.

In pen and field trials, Hemicell® has been shown to:reduce mortality;improve growth performance;reduce coccidial lesion scores in broilers;reduce wet feces in poultry;increase IGF-1 secretion in broilers.

Meta-analysis results from four EU studies in broilers in which the recommended dose of Hemicell® feed enzyme was fed resulted in these effects in 42 days (figure 3):

4.2 % increase in weight gain (P<0.0001)3.6 % improvement in feed conversion ratio (7.0 points; P<0.0004), and7.5 % improvement in EPEF (P<0.0001).

•••••

•••

Hemicell® reduces variability, measured as coefficient of variation (CV) of individual body weight, by most acutely improving the performance of the smallest animals in the population. The typical nature of the improvement on flock uniformity by Hemicell® is graphically represented in figure 2. The typical results summarized from many studies in different regions in table 1 shows that Hemicell® reduces CV of body weight by 12 to 26%. Improved uniformity increases process yield, carcass quality and productivity of broilers, breeders and layers.

56,7b

59,1a

109,1109,5

1.93b

1.86a

281b

302a

ADG,g/d ADFI, g/d FCR,g/g EPEF

Control Hemicell®

110

108

106

104

102

100

98

96

94

92

90C,

Contr

ol=

100

bENEFITS

Figure 1: Effect of Hemicell® on 0-45 day broiler performance (Parc Institure, USA). High ME=Low ME + 143 Kcal/kg in starter, grower and finisher diets.

Figure 2: Effect of Hemicell® on body weight uni-formity of 49-day old broilers (PARC Institute, Maryland, USA). Hemicell® reduces CV of body weight from 13.31 % to 11.02 % (P< 0.05).

Table 1: Summary of Hemicell® effect on body weight unifor-mity of broilers and meat ducks.1 Peterson/AA;Summary of 5 companies, USA, 19932 PARC Institute, MD USA, 1998 3 MAFIC, Beijing, China, 20024 Beijing, China, 1995.

Figure 3: Effect of Hemicell® on broiler performance (meta analysis from 4 EU efficacy studies)

2.08

2.12

2.16

2.2

2.24

1.76

1.8

1.84

1.88

1.92

P<0.05

ab

abb

c

a

ab

c

Wei

ght G

ain,

kg

Feed

Gai

n, k

g

High ME+Hemicell

High ME Low ME+Hemicell

Low ME

the only enzyme for β-mannan degradation

0

40

80

120

160

1000 1500 2000 2500 3000 3500 4000

Control HemicellCV 13.31 11.02N 905 967P < 0,05

Control

Hemicell

Weight Range (Grams)

Frequency