(a) SonoVue™

(b) Definity™

-35

-30

-25

-20

-15

-10

0 1 2 3 4 5 6 7

Zeta

Po

ten

tial, ζ

(m

V)

Diameter (µm)

Uncoated

Coated

-15

-10

-5

0

5

0 1 2 3 4 5 6 7

Ze

ta P

ote

nti

al, ζ

(m

V)

Diameter (µm)

Surface Charge Measurement of SonoVue™, Definity™ and

Optison™: a comparison of Laser Doppler Electrophoresis

and Micro-Electrophoresis

by F. Ja’afar, C.H. Leow, V. Garbin, C.A. Sennoga, M.X. Tang and J.M.

Seddon

Supplementary Material 3 – Zeta potential versus microbubble (MB) diameter for

SonoVue™ and Definity™. This figure shows the difference between zeta potential

readings between the Lipidure™-CM5206 coated channels and the uncoated

channels. As expected, the zeta potential values obtained for the uncoated

channels show a lower zeta potential value as MBs stick to microchannels. No

data was shown for Optison™ as MBs completely stick to microchannels, showing

no movement under electrophoresis.