Supplementary Figure S1.

Ki67

p-ST

AT3

Clea

ved

Casp

ase

3

DMSO Cobimetinib Ponatinib Cobi+Ponatinib DMSO Cobimetinib Ponatinib Cobi+Ponatinib

KP4 : 2D KP4 : 3D

DMSO Cobimetinib (1μM) GDC-0941 (1μM)

Han

ging

Dro

p C

ultu

reSo

ft A

gar

Cul

ture

Boile

d Se

rum

Cul

ture

0 0.01 0.10 1.00 10.000

50

100

150

% C

ell V

iabi

lity

Drug (μM)

GDC-0941Cobimetinib

0 0.01 0.10 1.00 10.000

200

400

600

800

1000

Sphe

roid

Dia

met

er (μ

m) GDC-0941

Cobimetinib

Hanging Drop Culture

0 0.01 0.10 1.00 10.000

50

100

150

% C

ell V

iabi

lity

GDC-0941Cobimetinib

0 0.01 0.10 1.00 10.000

50

100

150

Drug (μM)

GDC-0941 (μM)

KP4 : 2DKP4 : 3D

0 0.01 0.10 1.00 10.000

50

100

150

Cobimetinib (μM)

% C

ell V

iabi

lity

% C

ell V

iabi

lity

KP4 : 2DKP4 : 3D

Soft Agar Culture

Boiled Serum Culture

0 0.01 0.10 1.00 10.000

100

200

300

400

Sphe

roid

Dia

met

er (μ

m) GDC-0941

Cobimetinib

Boiled Serum Culture

Drug (μM)

Drug (μM)

A

B C

Supplementary Data

Supplementary Figure S1. KP4-derived 3D spheroids with histopathological

characteristics of micrometastases show differential sensitivity to small molecule

inhibitors (A) KP4 cells were cultured in RPMI media containing 10% normal FBS (for

2D culture) or 10% pre-boiled FBS (for 3D culture) for 3 days and IHC was performed

on 2D cell pellets or 3D spheroids for Ki67, p-STAT3 and cleaved caspase-3. (B)

Spheroids formed using 10% pre-boiled FBS show a similar drug sensitivity profile as

seen with other conventional 3D culture methods. (C) Cell viability assay and colony

count assay of spheroids formed from different 3D culture methods.

KP4(Bliss Score = 224.84)

1.0

0.8

0.6

0.4

0.2

0.0

10

3

10.3

0.1

0.03

0

00.

001

0.00

30.

010.

03 0.1

0.3 1 3 10

MIA-PACA2(Bliss Score = 234.98)

CAPAN-2(Bliss Score = 66.29)

ASPC-1(Bliss Score = 283.51)

SUIT-2(Bliss Score = 147.69)

BxPC3(Bliss Score = 68.12)

KPP cell line #1(Bliss Score = 503.10)

KPP cell line #2(Bliss Score = 348.85)

10

3

10.3

0.1

0.03

0

10

3

1

0.3

0.1

0.03

0

00.

001

0.00

30.

010.

03 0.1

0.3 1 3 10

00.

001

0.00

30.

010.

03 0.1

0.3 1 3 10

00.

001

0.00

30.

010.

03 0.1

0.3 1 3 10

00.

001

0.00

30.

010.

03 0.1

0.3 1 3 10 0

0.00

10.

003

0.01

0.03 0.

10.

3 1 3 10 00.

001

0.00

30.

010.

03 0.1

0.3 1 3 10 0

0.00

10.

003

0.01

0.03 0.

10.

3 1 3 1010

3

10.3

0.1

0.03

0

10

3

10.3

0.1

0.03

0

10

3

1

0.3

0.1

0.03

0

10

3

10.3

0.1

0.03

0

10

3

10.3

0.1

0.03

0

Pona

tinib

Pona

tinib

Pona

tinib

Pona

tinib

Pona

tinib

Pona

tinib

Pona

tinib

Cobimetinib Cobimetinib Cobimetinib Cobimetinib

Cobimetinib Cobimetinib Cobimetinib Cobimetinib

Rapa

myc

inG

DC-

0980

KP4(Bliss Score = 240.59)

KP4(Bliss Score = 500.25)

10

3

10.3

0.1

0.03

0

10

3

10.3

0.1

0.03

0

00.

001

0.00

30.

010.

03 0.1

0.3 1 3 10

Cobimetinib

00.

001

0.00

30.

010.

03 0.1

0.3 1 3 10

Cobimetinib

Pona

tinib

1.0

0.8

0.6

0.4

0.2

0.0

DMSOCobi150

100

50

0

150

100

50

0

150

100

50

0

30

20

10

1.5

1.0

0.5

0.0

150

100

50

0

Dox

o�

Dox

on

Dox

o�

Dox

on

KP4+shSTAT3 #2

**

* *

siNTC

siPDGFRα #1

siPDGFRα #2*

DM

SO

Cobi

shST

AT3

shST

AT3+

Cobi

** * * **

* ** **

siNTC

siPDGFRα #1

siPDGFRα #2

DM

SO

Cobi

shST

AT3

shST

AT3+

Cobi

siN

TC

siPD

GFR

α #1

siPD

GFR

α #2

**

DM

SO

Cobi

(1μM

)

DM

SO

Cobi

(1μM

)

DM

SOKP

4+sh

STAT

3 #2

KP4+

shST

AT3

#2

KP4

(Dox

o�

)

(Dox

on)

DM

SO

Cobi

met

inib

Pona

tinib

Das

atin

ib

Imat

inib

Nilo

tinib

Cobi

+Pon

Cobi

+Das

a

Cobi

+Im

a

Cobi

+Nilo

*

p-STAT3

β-Actin

% C

ell V

iabi

lity

% C

ell V

iabi

lity

% C

ell V

iabi

lity

% C

ell V

iabi

lity

% C

ell V

iabi

lity

RQ (G

APD

H n

orm

aliz

ed)

AA B

C

D

Supplementary Figure S2

Supplementary Figure S2. Cobimetinib/ponatinib co-treatment increases death of

pancreatic cancer cells (A) CTG assay measuring the effect of Dox-inducible STAT3

knockdown in combination with 1µM cobimetinib on KP4 cell viability; western blot

confirming efficient knockdown of STAT3. (B) CTG assay measuring the effect of

PDGFRα +/- STAT3 knockdown in combination with 1µM cobimetinib on KP4 and

MIA-PACA2 cell viability; validation of siPDGFRα knockdown using QRT-PCR. (C)

Effect of kinase inhibitors ponatinib, dasatinib, imatinib or nilotinib in combination with

cobimetinib on KP4 cell viability was measured using the CTG assay. (D) Bliss analysis

of synergy between cobimetinib and ponatinib / rapamycin / GDC-0980 was analyzed in

pancreatic cancer cell lines.

p-Er

kp-

STAT

3Vehicle Cobimetinib Ponatinib Cobi+PonKPP cells:

0 4 7 11 14-30-20-10

0102030

Body

Wei

ght (

% C

hang

e)

VehicleCobimetinibPonatinibCobi+Ponatinib

0 4 7 11 14 17-20

-10

0

10

20

VehicleCobimetinibPonatinibCobi+Ponatinib

Days Days

Body

Wei

ght (

% C

hang

e)

KP4 cells KPP cells

Supplementary Figure S3

A

B

Vehicle

Cobimetin

ib

Ponatinib

Cobi+Pon

0

50

100

150

VEG

F (p

g/m

l)

Vehicle

Cobimetin

ib

Ponatinib

Cobi+Pon

0

20

40

60

80

FGF

basi

c (p

g/m

l)

C KP4 cells KP4 cells

p-PDGFRα

p-STAT3

t-STAT3

p-S6

t-S6

Cl.PARP

β-Actin

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

Vehicle Cobimetinib Ponatinib Cobi+Pon

KPP xenograft tumors

D

Supplementary Figure S3. Cobimetinib/ponatinib co-treatment impairs tumor

growth in KPP xenografts (A) Representative tumor sections from KPP xenografts

examined with p-Erk and p-STAT3 antibodies by IHC. Scale bar: 20µm for IHC slides

(B) Change in body weight of mice treated with cobimetinib+/- ponatinib in KP4 and

KPP xenograft models. (C) Luminex assay of growth factors of plasma samples of KP4

xenograft mice. (D) Immunoblot validating activation of PDGFRa, S6 and STAT3 upon

cobimetinib/Ponatinib treatment of KPP xenograft tumors.

Supplementary Table S1. List of small molecule inhibitors used in drug screen

Small Molecule Inhibitor Target PubChem ID

Allopurinol Approved drug in oncology 2094

Anastrozole Approved drug in oncology 2187

Bortezomib Approved drug in oncology 387447

Celecoxib Approved drug in oncology 2662

Chloroquine HCl Approved drug in oncology 11717374

Dasatinib Approved drug in oncology 3062316

Exemestane Approved drug in oncology 60198

Fulvestrant Approved drug in oncology 104741

Imatinib Approved drug in oncology 5291

Imiquimod Approved drug in oncology 57469

Lenalidomide Approved drug in oncology 216326

Mechlorethamine Approved drug in oncology 4033

Methoxsalen Approved drug in oncology 4114

Mitotane Approved drug in oncology 4211

MTX Approved drug in oncology 126941

Taxol Approved drug in oncology 3081898

Quinacrine Approved drug in oncology 237

SAHA Approved drug in oncology 16729200

Tamoxifen Approved drug in oncology 2733526

Temozolomide Approved drug in oncology 5394

Teniposide Approved drug in oncology 107642

VM-26 Approved drug in oncology 101670240

Toremifene Approved drug in oncology 3005573

Vandetanib Approved drug in oncology 3081361

Pamidronate Approved drug in oncology 73351

Zoledronate Approved drug in oncology 70695671

Streptozocin Approved drug in oncology 29327

Goserelin acetate Approved drug in oncology 16052011

Alprazolam Approved drug in non-oncology 2118

Calcipotriene Approved drug in non-oncology 5282134

Darifenacin Hydrobromide Approved drug in non-oncology 444030

Digitoxin Approved drug in non-oncology 441207

Diltiazem HCL Approved drug in non-oncology 12449426

Fenofibrate Approved drug in non-oncology 3339

Fluvastatin Approved drug in non-oncology 3403

Hydrocortisone Approved drug in non-oncology 5754

Ibandronate Approved drug in non-oncology 60851

Ibuprofen Approved drug in non-oncology 3672

Ramipril Approved drug in non-oncology 5362129

Sisomicin Approved drug in non-oncology 36119

Sulindac Approved drug in non-oncology 1548887

Sumatriptan Succinate Approved drug in non-oncology 59772

Telmisartan Approved drug in non-oncology 65999

Triamcinolone acetonide Approved drug in non-oncology 6436

Vardenafil Approved drug in non-oncology 110634

Warfarin Approved drug in non-oncology 54678486

Metformin Hydrochloride Approved drug in non-oncology 14219

Diltiazem hydrochloride Approved drug in non-oncology 62920

Dexamethasone Approved drug in non-oncology 5743

SN-38 DNA and RNA synthesis inhibitor 57347736

Altretamine DNA damaging agent 2123

Azacitidine DNA damaging agent 9444

Bleomycin DNA damaging agent 456190

Busulfan DNA damaging agent 2478

Camptothecin DNA damaging agent 24360

Capecitabine DNA damaging agent 60953

Carmustine DNA damaging agent 2578

Chlorambucil DNA damaging agent 2708

Cisplatin DNA damaging agent 2767

Cladribine DNA damaging agent 20279

Clofarabine DNA damaging agent 119182

Cyclophosphamide DNA damaging agent 2907

Cytarabine DNA damaging agent 6253

Dacarbazine DNA damaging agent 5351166

Docetaxel DNA damaging agent 148124

Doxorubicin DNA damaging agent 31703

Epirubicin DNA damaging agent 41867

Floxuridine DNA damaging agent 5790

Fludarabine DNA damaging agent 657237

5-Fluorouracil DNA damaging agent 3385

Gemcitabine DNA damaging agent 60750

Hydroxyurea DNA damaging agent 3657

Idarubicin DNA damaging agent 42890

Ifosfamide DNA damaging agent 3690

Irinotecan DNA damaging agent 60838

Lomustine DNA damaging agent 3950

Melphalan DNA damaging agent 460612

Mercaptopurine, 6-MP DNA damaging agent 21651427

Mitomycin C DNA damaging agent 5746

Oxaliplatin DNA damaging agent 5310940

Pemetrexed disodium DNA damaging agent 53396302

Procarbazine DNA damaging agent 4915

Puromycin DNA damaging agent 439530

Thalidomide DNA damaging agent 5426

Thioguanine DNA damaging agent 2723601

Vinorelbine DNA damaging agent 5672

Etoposide phosphate DNA damaging agent 6918092

Topotecan DNA damaging agent 60700

Actinomycin D DNA damaging agent 2019

Decitabine DNA damaging agent 451668

Pentostatin DNA damaging agent 439693

Vinblastine DNA damaging agent 13342

Vincristine DNA damaging agent 5978

Carboplatin DNA damaging agent 426756

NU7026 DNA-PK inhibitor 9860529

Mometasone Furoate Corticosteroid 441336

AM114 20S proteosome inhibitor 11639443

MG132 26S proteasome inhibitor 462382

Carvedilol Beta blocker 2585

Donepezil hydrochloride Cholinesterase inhibitor 5741

Desloratadine Histamine H1-receptor antagonist 124087

Bicalutamide Nonsteroidal antiandrogen 2375

Tadalafil Phosphodiesterase inhibitor 110635

Omeprazole Proton pump inhibitor 4594

Raloxifene Estrogen receptor modulator 5035

Sertraline HCl Serotonin reuptake inhibitor 63009

Atorvastatin, Calcium Salt HMG-CoA reductase inhibitor. 71311905

Captopril ACE inhibitor 44093

Enalapril maleate ACE inhibitor 5388961

Felodipine ACE inhibitor 3333

Akt inhibitor 1/2 Akt inhibitor 24891133

GDC-0068 Akt inhibitor 24788740

Tamsulosin, Hydrochloride Alpha blocker 5362376

Irbesartan Angiotensin II receptor blocker 3749

Letrozole Aromatase inhibitor 3902

Rosuvastatin calcium HMG-CoA reductase inhibitor 656605

BAY 11-7082 Inhibitor of cytokine induced IKB-

alpha

5353431

Valsartan Angiotensin II receptor antagonist 60846

Bexarotene Anti-neoplastic agent 82146

Ezetimibe Antihyperlipidemic agent 150311

Ganciclovir Antiviral for CMV infections 3454

Ku55933 ATM Inhibitor 5278396

VX-680 Aurora kinase Inhibitor 5494449

Sorafenib Tyrosine Kinase inhibitor 216239

Nilotinib Bcr-Abl, c-Kit, PDGFR inhibitor 644241

Cytochalasin D Inhibits association and

dissociation of actin subunits

30956

Diazepam Binds to GAGAa, agonist 3016

PLX4032 BRAF Inhibitor 42611257

PD166793 MMP inhibitor 71311869

Mibefradil dihydrochloride

hydrate

c-Met inhibitor 60662

z-VAD-FMK Ca2+ channel blocker 5497174

RO-3306 Caspase Inhibitor 71433937

GW8510 CDK1 Inhibitor 6842391

Flavopiridol CDK2, CDK9 inhibitor 5459219

CHIR-265 Chiron Raf inhibitor 11656518

PD-407824 Chk1 and Wee1 inhibitor 4369491

Fluticasone Propionate Corticosteroid 444036

Tenidap COX/5-LOX inhibitor 54683953

Zebularine Cytidine deaminase inhibitor 100016

Erlotinib EGFR inhibitor 176870

Gefitinib EGFR inhibitor 123631

Tunicamycin ER stress inducer 11104835

Lapatinib ERbB-2 and EGFR dual kinase

inhibitor

208908

PD173074 FGFR inhibitor 1401

Trichostatin A HDAC inhibitor 444732

17-DMAG Hsp90 inhibitor 57339150

Mevastatin Hypolipidemic agent 64715

IKK-16 IKK inhibitor 56972179

FK-506 Immunosuppressant 6473866

Eeyarestatin 1 Inhibitor of ERAD 23947580

FTI-277 Inhibitor of H- and K-Ras

farnesyltransferase

3005532

Thapsigargin Inhibitor of SERCA 446378

Forskolin Inhibits adenylate cyclase 47936

Roscovitine Inhibits CDK1/CyclinB, CDK2,

CDK5

160355

BML-259 Inhibits cdk5, cdk2 16760027

Folinic Acid Inhibits DHFR, Enhances 5-FU. 135548

Brefeldin A Inhibits golgi transport 657314

Lovastatin Inhibits HMG-CoA reductase 16760544

U0126 Inhibits MEK1 and MEK2 3006531

Lonidamine Inhibits mitochondrial hexokinase 102115086

Blebbistatin Inhibits nonmuscle myosin II

ATPase

3476986

SB 202190 Inhibits p38 MAP kinases 353940

Nocodozole Inhibits polymerization of

microtubules

44354620

PP1 Inhibits Src fmaily of tyrosine

kinases

1400

Staurosporine Kinase inhibitor 5279

Nutlin-3 MDM2 inhibitor 216345

PD98059 MEK / ERK inhibitor 4713

GDC-0623 MEK inhibitor 42642654

XL-518 MEK inhibitor 16222096

AZD6244 MEK inhibitor 10127622

PD0325901 MEK inhibitor 9826528

Monastrol Mitotic agent 2987927

Rapamycin mTOR inhibitor 5040

Solifenacin Hydrochloride Muscarinic receptor antagonist 154058

Pindolol Non-specific beta-blocker 4828

Vioxx NSAID 5090

Clopidogrel Hydrogen

Sulfate

P2Y12 receptor antagonist 16757820

3-aminobenzamide PARG Inhibitor 53395946

Sildenafil citrate PDE inhibitor 62853

GDC-0980 PI3K inhibitor 25254071

G38390-45-3 PI3K inhibitor 443324

GDC-0941 PI3Ki pan-class I 17755052

H89 PKA Inhibitor 449241

UCN-01 PKC inhibitor 72271

Anagrelide HCI PDE3 inhibitor 2182

GDC-0879 Raf inhibitor 11717001

Megestrol acetate Progesterone derivative 11683

Lactacystin Proteasome Inhibitor 6610292

Esomeprazole magnesium Proton pump inhibitor 130564

Lansoprazole Proton pump inhibitor 3883

L-744,832 Ras farnesyltranferase inhibitor 16760531

Sunitinib RTK inhibitor 5329102

CGK733 Selective inhibitor of ATR and

ATM kinases

6605258

Chymostatin Serine and cysteine protease

inhibitor

443119

Citalopram Oxzlate Serotonin reuptake inhibitor 71314878

Allocholic Acid Steroid 160636

Progesterone Steroid 5994

BAY 61-3606 Syk inhibitor 10200390

Rosiglitazone maleate Thiazolidinedione 5281055

Pioglitazone Hydrochloride Thiazolidinedione 60560

Tandutinib Type III RTK inhibitor 3038522

Flurofamide Urease inhibitor 51173

Supplementary Table S2. Genotype with key mutations of cell lines used in this

study

CellLine KRAS TP53 CDKN2A/p16 PIK3CA PTEN SMAD4

mutation/deletion

mutation/deletion

mutation/deletion

mutation/deletion

mutation/deletion

mutation/deletion

KP4 12G>D WT Homdel WT WT HomdelMIA-PACA2 12G>C 248R>W Homdel WT WT WTCFPAC-1 12G>V 242C>R WT WT WT HomdelCAPAN-2 12G>V WT WT WT WT WTSUIT-2 12G>D 273R>H WT WT WT Homdel

ASPC-1 12G>D 135C>X Hemidel,78L>X WT WT

Hemidel,100R>T

PANC-1 12G>D 273R>H Homdel WT WT WTBxPC-3 WT 220Y>C Homdel WT WT Homdel

PATU-8988T 12G>V 282R>W WT WT WT Homdel

Supplementary Table S3. Tumor growth inhibition (%TGI) of KP4 and KPP

xenografts

KP4 xenografts Treatment

n/group n/last day Tumor volume (last day)

%TGI (AUC vs. Vehicle)

%TGI 95th percentile Upper UI

%TGI 95th percentile Lower UI

TTP 2X

Vehicle 10 10 1316 0 0 0 6

Ponatinib (30mg/kg, PO, QD)

10 9 408 56 -22 87 8.5

Cobimetinib (5mg/kg, PO, QD)

10 10 532 70 7 97 13

Cobi (5mg/kg, PO, QD)+Pon (30mg/kg, PO, QD)

10 7 217 103 76 123 NA

KPP xenografts Treatment

n/group n/last day

Tumor volume (last day)

%TGI (AUC vs. Vehicle)

%TGI 95th percentile Upper UI

%TGI 95th percentile Lower UI

TTP 2X

Vehicle 10 10 1305 0 0 0 4.5

Ponatinib (30mg/kg, PO, QD)

10 10 1045 29 3 50 7

Cobimetinib (5mg/kg, PO, QD)

10 10 917 31 5 50 6.5

Cobi (5mg/kg, PO, QD)+Pon (30mg/kg, PO, QD)

10 10 576 71 60 80 12.5

AUC = Area Under the Curve UI = Uncertainty Interval TTP = Time to Tumor Progression (2X original volume) Supplementary Table S4. TCGA microarray data for normal and PDAC tissues

PDGFRα Log2(Probe Intensity)

Gene_id Probeset Sample Type

Tissue Group

Tissue Diagnosis Tissue Diagnosis

Group8.794943 GeneID:5156 203131_at Tissue Pancreas Normal pancreas Normal9.279793 GeneID:5156 203131_at Tissue Pancreas Normal pancreas Normal9.227017 GeneID:5156 203131_at Tissue Pancreas Normal pancreas Normal8.889409 GeneID:5156 203131_at Tissue Pancreas Normal pancreas Normal8.958932 GeneID:5156 203131_at Tissue Pancreas Normal pancreas Normal8.993035 GeneID:5156 203131_at Tissue Pancreas Normal pancreas Normal10.39963 GeneID:5156 203131_at Tissue Pancreas Normal pancreas Normal10.50218 GeneID:5156 203131_at Tissue Pancreas Normal pancreas Normal9.184865 GeneID:5156 203131_at Tissue Pancreas Normal pancreas Normal8.808953 GeneID:5156 203131_at Tissue Pancreas Normal pancreas Normal7.621978 GeneID:5156 203131_at Tissue Pancreas Normal pancreas Normal8.470824 GeneID:5156 203131_at Tissue Pancreas Normal pancreas Normal8.586969 GeneID:5156 203131_at Tissue Pancreas Normal pancreas Normal7.883403 GeneID:5156 203131_at Tissue Pancreas Normal pancreas Normal8.024827 GeneID:5156 203131_at Tissue Pancreas Normal pancreas Normal8.687401 GeneID:5156 203131_at Tissue Pancreas Normal pancreas Normal9.584143 GeneID:5156 203131_at Tissue Pancreas Normal pancreas Normal9.421285 GeneID:5156 203131_at Tissue Pancreas Adenocarcinoma Cancer10.09945 GeneID:5156 203131_at Tissue Pancreas Adenocarcinoma Cancer10.1031 GeneID:5156 203131_at Tissue Pancreas Adenocarcinoma Cancer

10.92539 GeneID:5156 203131_at Tissue Pancreas Adenocarcinoma Cancer11.56405 GeneID:5156 203131_at Tissue Pancreas Adenocarcinoma Cancer8.581601 GeneID:5156 203131_at Tissue Pancreas Adenocarcinoma Cancer10.75649 GeneID:5156 203131_at Tissue Pancreas Adenocarcinoma Cancer10.77969 GeneID:5156 203131_at Tissue Pancreas Adenocarcinoma Cancer10.46082 GeneID:5156 203131_at Tissue Pancreas Adenocarcinoma Cancer9.26923 GeneID:5156 203131_at Tissue Pancreas Adenocarcinoma Cancer11.24358 GeneID:5156 203131_at Tissue Pancreas Adenocarcinoma Cancer10.43788 GeneID:5156 203131_at Tissue Pancreas Adenocarcinoma Cancer10.45637 GeneID:5156 203131_at Tissue Pancreas Adenocarcinoma Cancer10.82082 GeneID:5156 203131_at Tissue Pancreas Adenocarcinoma Cancer10.61548 GeneID:5156 203131_at Tissue Pancreas Adenocarcinoma Cancer10.05359 GeneID:5156 203131_at Tissue Pancreas Adenocarcinoma Cancer10.88377 GeneID:5156 203131_at Tissue Pancreas Adenocarcinoma Cancer10.03931 GeneID:5156 203131_at Tissue Pancreas Adenocarcinoma Cancer10.43891 GeneID:5156 203131_at Tissue Pancreas Adenocarcinoma Cancer9.878045 GeneID:5156 203131_at Tissue Pancreas Adenocarcinoma Cancer10.63892 GeneID:5156 203131_at Tissue Pancreas Adenocarcinoma Cancer9.042114 GeneID:5156 203131_at Tissue Pancreas Adenocarcinoma Cancer10.11472 GeneID:5156 203131_at Tissue Pancreas Adenocarcinoma Cancer9.814941 GeneID:5156 203131_at Tissue Pancreas Adenocarcinoma Cancer10.32152 GeneID:5156 203131_at Tissue Pancreas Adenocarcinoma Cancer9.091601 GeneID:5156 203131_at Tissue Pancreas Adenocarcinoma Cancer9.222542 GeneID:5156 203131_at Tissue Pancreas Adenocarcinoma Cancer10.70983 GeneID:5156 203131_at Tissue Pancreas Adenocarcinoma Cancer8.636107 GeneID:5156 203131_at Tissue Pancreas Adenocarcinoma Cancer10.39972 GeneID:5156 203131_at Tissue Pancreas Adenocarcinoma Cancer7.513814 GeneID:5156 203131_at Tissue Pancreas Adenocarcinoma Cancer9.729338 GeneID:5156 203131_at Tissue Pancreas Adenocarcinoma Cancer9.566706 GeneID:5156 203131_at Tissue Pancreas Adenocarcinoma Cancer8.181587 GeneID:5156 203131_at Tissue Pancreas Adenocarcinoma Cancer8.138516 GeneID:5156 203131_at Tissue Pancreas Adenocarcinoma Cancer8.845604 GeneID:5156 203131_at Tissue Pancreas Adenocarcinoma Cancer10.00465 GeneID:5156 203131_at Tissue Pancreas Adenocarcinoma Cancer

PDGFRβ Log2(Probe Intensity)

Gene_id Probeset Sample Type

Tissue Group

Tissue Diagnosis Tissue Diagnosis

Group7.556889 GeneID:5159 202273_at Tissue Pancreas Normal pancreas Normal5.260128 GeneID:5159 202273_at Tissue Pancreas Normal pancreas Normal7.409171 GeneID:5159 202273_at Tissue Pancreas Normal pancreas Normal6.764048 GeneID:5159 202273_at Tissue Pancreas Normal pancreas Normal7.422604 GeneID:5159 202273_at Tissue Pancreas Normal pancreas Normal7.341148 GeneID:5159 202273_at Tissue Pancreas Normal pancreas Normal8.150971 GeneID:5159 202273_at Tissue Pancreas Normal pancreas Normal8.320784 GeneID:5159 202273_at Tissue Pancreas Normal pancreas Normal7.788783 GeneID:5159 202273_at Tissue Pancreas Normal pancreas Normal7.156454 GeneID:5159 202273_at Tissue Pancreas Normal pancreas Normal6.155598 GeneID:5159 202273_at Tissue Pancreas Normal pancreas Normal6.59976 GeneID:5159 202273_at Tissue Pancreas Normal pancreas Normal

6.819837 GeneID:5159 202273_at Tissue Pancreas Normal pancreas Normal5.95445 GeneID:5159 202273_at Tissue Pancreas Normal pancreas Normal

6.956264 GeneID:5159 202273_at Tissue Pancreas Normal pancreas Normal6.647721 GeneID:5159 202273_at Tissue Pancreas Normal pancreas Normal8.160778 GeneID:5159 202273_at Tissue Pancreas Normal pancreas Normal7.726896 GeneID:5159 202273_at Tissue Pancreas Adenocarcinoma Cancer7.46055 GeneID:5159 202273_at Tissue Pancreas Adenocarcinoma Cancer

8.502994 GeneID:5159 202273_at Tissue Pancreas Adenocarcinoma Cancer9.322616 GeneID:5159 202273_at Tissue Pancreas Adenocarcinoma Cancer9.374715 GeneID:5159 202273_at Tissue Pancreas Adenocarcinoma Cancer7.797891 GeneID:5159 202273_at Tissue Pancreas Adenocarcinoma Cancer8.995366 GeneID:5159 202273_at Tissue Pancreas Adenocarcinoma Cancer9.644044 GeneID:5159 202273_at Tissue Pancreas Adenocarcinoma Cancer8.891796 GeneID:5159 202273_at Tissue Pancreas Adenocarcinoma Cancer8.50103 GeneID:5159 202273_at Tissue Pancreas Adenocarcinoma Cancer

9.229039 GeneID:5159 202273_at Tissue Pancreas Adenocarcinoma Cancer8.961214 GeneID:5159 202273_at Tissue Pancreas Adenocarcinoma Cancer9.122539 GeneID:5159 202273_at Tissue Pancreas Adenocarcinoma Cancer8.841867 GeneID:5159 202273_at Tissue Pancreas Adenocarcinoma Cancer8.608741 GeneID:5159 202273_at Tissue Pancreas Adenocarcinoma Cancer8.56492 GeneID:5159 202273_at Tissue Pancreas Adenocarcinoma Cancer

8.615495 GeneID:5159 202273_at Tissue Pancreas Adenocarcinoma Cancer8.229863 GeneID:5159 202273_at Tissue Pancreas Adenocarcinoma Cancer8.591798 GeneID:5159 202273_at Tissue Pancreas Adenocarcinoma Cancer9.707525 GeneID:5159 202273_at Tissue Pancreas Adenocarcinoma Cancer8.560279 GeneID:5159 202273_at Tissue Pancreas Adenocarcinoma Cancer7.758313 GeneID:5159 202273_at Tissue Pancreas Adenocarcinoma Cancer7.691537 GeneID:5159 202273_at Tissue Pancreas Adenocarcinoma Cancer8.339635 GeneID:5159 202273_at Tissue Pancreas Adenocarcinoma Cancer8.350952 GeneID:5159 202273_at Tissue Pancreas Adenocarcinoma Cancer7.564781 GeneID:5159 202273_at Tissue Pancreas Adenocarcinoma Cancer7.276369 GeneID:5159 202273_at Tissue Pancreas Adenocarcinoma Cancer8.803938 GeneID:5159 202273_at Tissue Pancreas Adenocarcinoma Cancer5.474688 GeneID:5159 202273_at Tissue Pancreas Adenocarcinoma Cancer7.747431 GeneID:5159 202273_at Tissue Pancreas Adenocarcinoma Cancer6.137455 GeneID:5159 202273_at Tissue Pancreas Adenocarcinoma Cancer8.855201 GeneID:5159 202273_at Tissue Pancreas Adenocarcinoma Cancer8.112997 GeneID:5159 202273_at Tissue Pancreas Adenocarcinoma Cancer7.976575 GeneID:5159 202273_at Tissue Pancreas Adenocarcinoma Cancer8.131375 GeneID:5159 202273_at Tissue Pancreas Adenocarcinoma Cancer7.670179 GeneID:5159 202273_at Tissue Pancreas Adenocarcinoma Cancer8.541986 GeneID:5159 202273_at Tissue Pancreas Adenocarcinoma Cancer

IL6-R Log2(Probe Intensity)

Gene_id Probeset Sample Type

Tissue Group

Tissue Diagnosis Tissue Diagnosis

Group5.553321 GeneID:3570 205945_at Tissue Pancreas Normal pancreas Normal4.120181 GeneID:3570 205945_at Tissue Pancreas Normal pancreas Normal3.985927 GeneID:3570 205945_at Tissue Pancreas Normal pancreas Normal4.267829 GeneID:3570 205945_at Tissue Pancreas Normal pancreas Normal4.991783 GeneID:3570 205945_at Tissue Pancreas Normal pancreas Normal3.940651 GeneID:3570 205945_at Tissue Pancreas Normal pancreas Normal4.715778 GeneID:3570 205945_at Tissue Pancreas Normal pancreas Normal6.031536 GeneID:3570 205945_at Tissue Pancreas Normal pancreas Normal5.290729 GeneID:3570 205945_at Tissue Pancreas Normal pancreas Normal4.473181 GeneID:3570 205945_at Tissue Pancreas Normal pancreas Normal4.173704 GeneID:3570 205945_at Tissue Pancreas Normal pancreas Normal3.933525 GeneID:3570 205945_at Tissue Pancreas Normal pancreas Normal5.157238 GeneID:3570 205945_at Tissue Pancreas Normal pancreas Normal4.945232 GeneID:3570 205945_at Tissue Pancreas Normal pancreas Normal3.684718 GeneID:3570 205945_at Tissue Pancreas Normal pancreas Normal3.924458 GeneID:3570 205945_at Tissue Pancreas Normal pancreas Normal4.979816 GeneID:3570 205945_at Tissue Pancreas Normal pancreas Normal4.631535 GeneID:3570 205945_at Tissue Pancreas Adenocarcinoma Cancer5.525271 GeneID:3570 205945_at Tissue Pancreas Adenocarcinoma Cancer5.343428 GeneID:3570 205945_at Tissue Pancreas Adenocarcinoma Cancer5.365025 GeneID:3570 205945_at Tissue Pancreas Adenocarcinoma Cancer6.389725 GeneID:3570 205945_at Tissue Pancreas Adenocarcinoma Cancer6.064351 GeneID:3570 205945_at Tissue Pancreas Adenocarcinoma Cancer4.978231 GeneID:3570 205945_at Tissue Pancreas Adenocarcinoma Cancer5.265962 GeneID:3570 205945_at Tissue Pancreas Adenocarcinoma Cancer4.801198 GeneID:3570 205945_at Tissue Pancreas Adenocarcinoma Cancer5.478386 GeneID:3570 205945_at Tissue Pancreas Adenocarcinoma Cancer6.81306 GeneID:3570 205945_at Tissue Pancreas Adenocarcinoma Cancer

6.182141 GeneID:3570 205945_at Tissue Pancreas Adenocarcinoma Cancer4.817283 GeneID:3570 205945_at Tissue Pancreas Adenocarcinoma Cancer5.62403 GeneID:3570 205945_at Tissue Pancreas Adenocarcinoma Cancer

5.553858 GeneID:3570 205945_at Tissue Pancreas Adenocarcinoma Cancer6.210491 GeneID:3570 205945_at Tissue Pancreas Adenocarcinoma Cancer5.7035 GeneID:3570 205945_at Tissue Pancreas Adenocarcinoma Cancer

5.551391 GeneID:3570 205945_at Tissue Pancreas Adenocarcinoma Cancer7.040005 GeneID:3570 205945_at Tissue Pancreas Adenocarcinoma Cancer4.326793 GeneID:3570 205945_at Tissue Pancreas Adenocarcinoma Cancer6.092874 GeneID:3570 205945_at Tissue Pancreas Adenocarcinoma Cancer5.327012 GeneID:3570 205945_at Tissue Pancreas Adenocarcinoma Cancer4.138626 GeneID:3570 205945_at Tissue Pancreas Adenocarcinoma Cancer5.365025 GeneID:3570 205945_at Tissue Pancreas Adenocarcinoma Cancer5.924268 GeneID:3570 205945_at Tissue Pancreas Adenocarcinoma Cancer6.036687 GeneID:3570 205945_at Tissue Pancreas Adenocarcinoma Cancer5.053131 GeneID:3570 205945_at Tissue Pancreas Adenocarcinoma Cancer6.451621 GeneID:3570 205945_at Tissue Pancreas Adenocarcinoma Cancer2.964388 GeneID:3570 205945_at Tissue Pancreas Adenocarcinoma Cancer4.699464 GeneID:3570 205945_at Tissue Pancreas Adenocarcinoma Cancer7.824091 GeneID:3570 205945_at Tissue Pancreas Adenocarcinoma Cancer6.540267 GeneID:3570 205945_at Tissue Pancreas Adenocarcinoma Cancer3.617126 GeneID:3570 205945_at Tissue Pancreas Adenocarcinoma Cancer5.350239 GeneID:3570 205945_at Tissue Pancreas Adenocarcinoma Cancer8.541102 GeneID:3570 205945_at Tissue Pancreas Adenocarcinoma Cancer5.96024 GeneID:3570 205945_at Tissue Pancreas Adenocarcinoma Cancer

6.767234 GeneID:3570 205945_at Tissue Pancreas Adenocarcinoma Cancer