190 Rev. Toxicol. (2014) 31: 187-195

Tatay E, Meca G, Font G, Ruiz MJ

Fig

ure

2. C

once

ntra

tion

-res

pons

e cu

rves

for i

ndiv

idua

l ZE

A (

), α

-ZO

L (

), β

-ZO

L (

) bi

nary

com

bina

tion

s (

)

and

tert

iary

com

bina

tion

s (

) of

them

in H

epG

2 ce

lls

afte

r 24

, 48

and

72 h

of

expo

sure

. Con

cent

rati

on –

resp

onse

cur

ves

wer

e fi

tted

by

non-

line

ar r

egre

ssio

n. D

ata

are

expr

esse

d as

mea

n va

lue

± S

D o

f in

depe

nden

t ex

peri

men

ts (

n=3)

. p≤

0.05

(*)

re

pres

ent s

igni

fica

nt d

iffe

renc

e fr

om c

ontr

ol.

Fig

ure

3. C

ombi

nati

on in

dex

(CI)

/fra

ctio

nal e

ffec

t cur

ve a

s de

scri

bed

by C

hou

and

Tal

alay

mod

el f

or H

epG

2 ce

ll e

xpos

ed to

ZE

A, α

-ZO

L a

nd β

-ZO

L b

inar

y an

d te

rtia

ry c

ombi

anti

ons.

Eac

h po

int r

epre

sent

s th

e C

I ±

SD

at a

frac

tion

al e

ffec

t as

dete

rmie

d in

our

exp

erim

ents

. The

dot

ted

line

indi

cate

s ad

diti

vity

, the

are

a un

der t

he d

otte

d li

ne s

yner

gy, a

nd th

e ar

ea a

bove

of

the

dott

ed li

ne

anta

goni

sm.

Hep

G2

cell

s w

ere

expo

sed

duri

ng 2

4, 4

8 an

d 72

h w

ith

ZE

A+

α-Z

OL

and

ZE

A+

β-Z

OL

at

mol

ar r

atio

of

2:1;

α-Z

OL

+β

-ZO

L a

t m

olar

rat

io o

f 1:

1 (e

quim

olar

pro

port

ion)

and

Z

EA

+α

-ZO

L+

β-Z

OL

at m

olar

rati

o of

2:1

:1.

191Rev. Toxicol. (2014) 31: 187-195

Cytotoxic and interactive effects of zearalenone, α-zearalenol and β-zearalenol and formation of metabolites in HepG2 cells