Reference Gene Panel Rat - TATAA Biocenter · TBP TATAA-box binding protein ARBP Acidic ribosomal...
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TATAA Biocenter AB Odinsgatan 28, 411 03 Göteborg Tel: +46 31 761 57 00, Fax: +46 31 15 28 90, E-mail: [email protected] Gene Full Name GAPDH Glyceraldehyde-3-phosphate dehydrogenase TUBB5 Tubulin, β-polypeptide PPIA Cyclophilin A ACTB β-Actin YWHAZ Tyrosine 3-/Tryptophan 5-monooxygenase activation protein, ζ-polypeptide RRN18S 18S rRNA B2M β-2-microglobulin PGK1 Phosphoglycerate kinase 1 TBP TATAA-box binding protein ARBP Acidic ribosomal protein P0 GUSB β-glucuronidase HPRT1 Hypoxanthine-guanine phosphoribosyltransferase Reference Gene Panel Rat Reference Gene Panel The genes included in the Reference Gene Panel Rat are commonly used in gene expression studies. Genes with varying cellular function and expression level have been selected. Primers have been designed to be exon-spanning where possible and to give low levels of primer-dimers. Materials included • Primer sets for 12 candidate reference genes - Assays for the most common reference genes for gene expression studies, provide a rapid and cost efficient way to identify your reference genes for Rat. Enough for 100 reactions per gene. • Positive Control DNA - Use the positive control DNA to verify that the assays work well with your favorite master mix. The assays have been validated for use with most commercially available master mixes for qPCR containing SYBR Green I. • GenEx Standard - Software including both GeNorm and Normfinder to select optimum reference genes. Including GenEx Standard Accession number and exon information can be requested from [email protected]
Transcript of Reference Gene Panel Rat - TATAA Biocenter · TBP TATAA-box binding protein ARBP Acidic ribosomal...
TATAA Biocenter AB Odinsgatan 28, 411 03 GöteborgTel: +46 31 761 57 00, Fax: +46 31 15 28 90, E-mail: [email protected]
Gene Full NameGAPDH Glyceraldehyde-3-phosphate dehydrogenaseTUBB5 Tubulin, β-polypeptidePPIA Cyclophilin AACTB β-ActinYWHAZ Tyrosine 3-/Tryptophan 5-monooxygenase activation protein, ζ-polypeptideRRN18S 18S rRNAB2M β-2-microglobulinPGK1 Phosphoglycerate kinase 1TBP TATAA-box binding proteinARBP Acidic ribosomal protein P0GUSB β-glucuronidaseHPRT1 Hypoxanthine-guanine phosphoribosyltransferase
Reference Gene Panel Rat
Reference Gene PanelThe genes included in the Reference Gene Panel Rat are commonly used in gene expression studies. Genes with varying cellular function and expression level have been selected. Primers have been designed to be exon-spanning where possible and to give low levels of primer-dimers.
Materials included• Primer sets for 12 candidate reference genes - Assays for the most common reference genes for gene expression studies, provide a rapid and cost efficient way to identify your reference genes for Rat. Enough for 100 reactions per gene.
• Positive Control DNA - Use the positive control DNA to verify that the assays work well with your favorite master mix. The assays have been validated for use with most commercially available master mixes for qPCR containing SYBR Green I.
• GenEx Standard - Software including both GeNorm and Normfinder to select optimum reference genes.
Including GenEx Standard
Accession number and exon information can be requested from [email protected]
TATAA Biocenter AB Odinsgatan 28, 411 03 GöteborgTel: +46 31 761 57 00, Fax: +46 31 15 28 90, E-mail: [email protected]
Reference Gene Panel RatBackgroundFor all gene expression studies using quantitative PCR it is necessary to compensate for differences between samples due to material losses, differences in RT yields and PCR inhibition. Normalization should include a reference gene, but can also be complemented by identical sample input amounts. The reference gene should have constant expression in all the samples compared. There is no universal reference gene, expressed at a constant level under all conditions and in all tissues, hence the choice of reference genes should be validated for each new study set up. The best way to choose the proper reference gene is by running a panel of potential genes on a number of representative test samples. The gene(s) most appropriate for normalization are chosen in each study. The Reference Gene Panel Rat consists of 12 validated qPCR assays for the most common reference genes.
GenEx StandardGenEx Standard is a newly developed software based on a highly user friendly windows platform. A complete data management system together with powerful tools like 2D and 3D graphics data visualization helps you editing and pre-processing real-time PCR data. Features for mail support, and viewlet live demonstrations make it very easy to get started. GenEx Standard includes both GeNorm and Normfinder to select optimum reference genes. With GenEx Standard you can always find the best gene for your system.
Storage and instructions for usePrimer solutions are supplied as ready-to-use working solutions in water and are stable for 1 month at +4oC and should be stored at -20oC for longer term storage. 1μl of primer solution per 25μl PCR is recommended. Recommended temperature protocol is 95oC 20s, 60oC 20s, 72oC 20s. Detection should be performed during/after elongation. Primer sets are experimentally validated for use with most commercially available mastermixes for qPCR containing SYBR Green I.
Contact and orderingThe Reference Gene Panel Rat can be ordered from our distribution partners around the world or in our webshop. Please visit www.tataa.com.
ReferencesVandesompele J. et al (2002) Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes Genome Biology 3(7) 0034.I - 0034.II
Szabo A. et al (2004) Statistical modeling for selecting housekeeper genes Genome Biology 5:R59
Andersen C.L. et al (2004) Normalization of Real-Time Quantitative Reverse Transcription-PCRData: A Model-Based Variance Estimation Approach to Identify Genes Suited for Normalization,Applied to Bladder and Colon Cancer Data Sets Cancer Research 64, 5245–5250
Including GenEx Standard
