Production Dynamics and Degradation of Novel Cyanopeptides · This project has received funding...
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This project has received funding from the European Union’s Horizon 2020 research and innovation programme under the Marie Sklodowska-Curie grant agreement No. 722493. Greifensee, Switzerland Baldeggersee, Switzerland Brno, Czech Republic Lake Varese, Italy Production Dynamics and Degradation of Novel Cyanopeptides WHO 1 μg/L MC-LR in drinking water
Transcript of Production Dynamics and Degradation of Novel Cyanopeptides · This project has received funding...
This project has received funding from the European Union’s Horizon 2020 research and innovation programme under the Marie Sklodowska-Curie grant agreement No. 722493.
Greifensee, Switzerland Baldeggersee, Switzerland
Brno, Czech Republic Lake Varese, Italy
Production Dynamics and Degradation
of Novel Cyanopeptides
WHO 1 μg/L MC-LR in drinking water
This project has received funding from the European Union’s Horizon 2020 research and innovation programme under the Marie Sklodowska-Curie grant agreement No. 722493.
Project Progress
Analytical workflow for > 700 suspects
Production Dynamics
Phototransformation
Biotransformation
Janssen E. M-L. (2019). “Cyanobacterial peptides beyond microcystins – Review on co-occurrence, toxicity and challenges for risk assessment. Water Research (151):
488-499.; Welker, M. and H. von Döhren (2006). "Cyanobacterial peptides - Nature's own combinatorial biosynthesis." Fems Microbiology Reviews 30(4): 530-563.
Emerging CyanopeptidesWho is abundant and persistent ?
This project has received funding from the European Union’s Horizon 2020 research and innovation programme under the Marie Sklodowska-Curie grant agreement No. 722493.
Who is abundant ?
Cyanopeptide profiles of bloom-forming species
Microcystis aeruginosa Dolichospermum flos-aquaePlanktothrix rubescens
cyclamidesmicrocystins
cyanopeptolins
anabaenopeptins
This project has received funding from the European Union’s Horizon 2020 research and innovation programme under the Marie Sklodowska-Curie grant agreement No. 722493.
Who is abundant ?
Cyanopeptide profiles of bloom-forming species
Microcystis aeruginosa Dolichospermum flos-aquaePlanktothrix rubescens
cyclamidesmicrocystins
cyanopeptolins
anabaenopeptins
one speciesproduces multiple
peptides
microcystins neveroccured alone and are not always dominating
This project has received funding from the European Union’s Horizon 2020 research and innovation programme under the Marie Sklodowska-Curie grant agreement No. 722493.
Microcystis aeruginosa
Who is abundant ?
Cyanopeptide profiles of bloom-forming species
peak productionduring exponential
growth phase
cyclamidesmicrocystins
cyanopeptolins
anabaenopeptins
0.0
0.2
0.4
0.6
0.8
1.0
1.2
1.4
1.6
0 10 20 30 40
ce
ll a
bu
nda
nce
in lo
g(C
t/C
0)
cultivation time (days)
lag exponential stationary
increase of cells over time cyanopeptides per cell
one speciesproduces multiple
peptides
microcystins neveroccured alone and are not always dominating
This project has received funding from the European Union’s Horizon 2020 research and innovation programme under the Marie Sklodowska-Curie grant agreement No. 722493.
Microcystis aeruginosa
Who is abundant ?
Cyanopeptide profiles of bloom-forming species
nutrient availability influences toxin concentrations
cyclamidesmicrocystins
cyanopeptolins
anabaenopeptins
peak productionduring exponential
growth phase
one speciesproduces multiple
peptides
microcystins neveroccured alone and are not always dominating
This project has received funding from the European Union’s Horizon 2020 research and innovation programme under the Marie Sklodowska-Curie grant agreement No. 722493.
Who is persistent?
Risk = Toxicity x Exposure
This project has received funding from the European Union’s Horizon 2020 research and innovation programme under the Marie Sklodowska-Curie grant agreement No. 722493.
Who is persistent?
Phototransformation
Risk = Toxicity x Exposure
simulated sunlight
This project has received funding from the European Union’s Horizon 2020 research and innovation programme under the Marie Sklodowska-Curie grant agreement No. 722493.
Microcystis aeruginosa
Greifensee
pH 9.05 mgc/L DOM
3 hrs
6 % removal
Who is persistent?
Phototransformation
This project has received funding from the European Union’s Horizon 2020 research and innovation programme under the Marie Sklodowska-Curie grant agreement No. 722493.
27 % removal
Dolichospermum flos-aquaePlanktothrix rubescens
21 % removal
6 % removal
Microcystis aeruginosa
Who is persistent?
Phototransformation
This project has received funding from the European Union’s Horizon 2020 research and innovation programme under the Marie Sklodowska-Curie grant agreement No. 722493.
Natumi, R., Janssen, E. M.-L. Phototransformation of
cyanobacterial peptides (in preparation).
MC-LR
[dAsp3]-MC-WR
MC-LW
Who is persistent?
Phototransformation – light absorbance
-2
-1.5
-1
-0.5
0
0 1 2 3
conce
ntr
ation in ln
(C/C
0)
sunlight exposure time (hours)
Half-lives (hrs)MC-LR n.d.[dAsp3]-MC-WR 3.0MC-LW 1.5
Dark control
MC-LR – cannot not absorb light
replace by tryptophan (W) absorbs light
This project has received funding from the European Union’s Horizon 2020 research and innovation programme under the Marie Sklodowska-Curie grant agreement No. 722493.
Natumi, R., Janssen, E. M.-L. Phototransformation of
cyanobacterial peptides (in preparation).
MC-LR
[dAsp3]-MC-WR
MC-LW
Who is persistent?
Phototransformation – light absorbance
-2
-1.5
-1
-0.5
0
0 1 2 3
conce
ntr
ation in ln
(C/C
0)
sunlight exposure time (hours)
Half-lives (hrs)MC-LR n.d.[dAsp3]-MC-WR 3.0MC-LW 1.5
Dark control
MC-LR – cannot not absorb light
replace by tryptophan (W) absorbs light
pH can influence light absorbance
0
1
10
100
6 7 8 9 10 11h
alf-
live
(h
rs)
pH
Half-lives (hrs)
pH 7 pH 12
48 hrs
9 hrs
This project has received funding from the European Union’s Horizon 2020 research and innovation programme under the Marie Sklodowska-Curie grant agreement No. 722493.
Future Work
1) Phototransformation photodegradation mechanism for abundant cyanopeptides
2) Secondment at Recetox in Czech Republic combine peptide analysis with toxicity tests
3) Biodegradation with river and lake communities
This project has received funding from the European Union’s Horizon 2020 research and innovation programme under the Marie Sklodowska-Curie grant agreement No. 722493.
Impact
Cyanobacterial Bloom Risk Assessment = Toxicity x Exposure
• Suspect list > 700 cyanopeptides
• Nutrient effects cyanopeptide production and profiles
Who is abundant?
Who is persistent?
Prioritize which potential cyanotoxin can reach drinking
water treatment plants and thus need further
attention
Acknowledgements
This project has received funding from the European Union’s Horizon 2020 research and innovation programme under the Marie Sklodowska-Curie grant agreement No. 722493.
Dr. Martin R. JonesPostdoc. researcher
Sandro Marcotullio
Evelyn Vonwyl
Master students
Dr. E. Janssensupervisor, Eawag
Environmental Chemistry team
at Eawag
NaToxAq Family
