Interaction between PARP-1 and HIF-2α in the hypoxic response
PARP
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GR PARP GAPDH Dex Treatment - + - + Antagomir Control miR-130b S4. MM.1Re cells were transfected with either control or anti-miR-130b antagomirs (10nM) for 24hrs then treated with 1μM Dex for an additional 48hrs. A. Whole cell lysates were then collected and analyzed by immunblot. B. Total RNA was collected and miR-130b expression levels measured. m iR-130b R elative E xpression 0 0.2 0.4 0.6 0.8 1 1.2 M M .1Re C ont M M .1R e C ontDex M M .1Re anti-130b M M .1Re anti-130b D ex C ellLine and Antagom ir RelativeQuantity A B
description
A. B. Antagomir Control miR-130b. Dex Treatment - + - +. GR. PARP. GAPDH. - PowerPoint PPT Presentation
Transcript of PARP
GR
PARP
GAPDH
Dex Treatment - + - +
Antagomir Control miR-130b
S4. MM.1Re cells were transfected with either control or anti-miR-130b antagomirs (10nM) for 24hrs then treated with 1μM Dex for an additional 48hrs. A. Whole cell lysates were then collected and analyzed by immunblot. B. Total RNA was collected and miR-130b expression levels measured.
miR-130b Relative Expression
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MM.1Re Cont MM.1Re Cont Dex MM.1Re anti-130b MM.1Re anti-130bDex
Cell Line and Antagomir
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tive
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ntity
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