Mouse and Human Dendritic Cell Subsets · F4/80 Integrin αE/CD103 ... Mouse and Human Dendritic...

Bone Marrow

Hematopoietic Stem CellCommon Myeloid Progenitor (CMP)

Granulocyte-Macrophage Progenitor (GMP)

Common Monocyte Progenitor (cMoP)

Monocyte

Plasmacytoid DC

Pre-DC

Macrophage Dendritic Progenitor

(MDP)

Common DendriticProgenitor (CDP)

Hematopoietic Stem Cell

Granulocyte-MacrophageProgenitor (GMP)

Multi-LymphoidProgenitor (MLP)

Human Equivalent ofMDP or CDP

Common MonocyteProgenitor (cMoP)

Monocyte

Plasmacytoid DC

Pre-DC

Blood DC

Pre-DC

CD16+ Monocyte

Pre-DC

Human Skin DCs

Mouse Skin DCs

Mouse Lymphoid Tissue DCs

Mouse Inflammatory DCsin Peripheral Tissues & Draining

Lymph Nodes During Inflammation

Mouse Intestinal Lamina Propria/Peyer’s Patch DCs

Mouse Lung/Liver/Kidney DCs

Human Blood DCs

Human SecondaryLymphoid Organs/Non-Lymphoid

Tissue Parenchyma DCs

Blood MonocyteBlood Monocyte

Plasmacytoid DCPlasmacytoid DC

Pre-DC

Inflammatory DC

Cell Surface MarkersCD4–

CD8α–

CD11c+

CD24+

CLEC9a+

CX3CR1–

DC-SIGN/CD209–

DEC-205/CD205+

EpCAM/TROP1–/low

F4/80–

Integrin αE/CD103+

Integrin αM/CD11b–/low

Langerin/CD207+

MHC class II+

SIRPα/CD172a–

XCR1+

Integrin αE/CD103+ CD11b– CD207+ Dermal DC

Cell Surface MarkersCD24+

EpCAM/TROP1–/low

Integrin αE/CD103–


Langerin/CD207+

MHC class II+

SIRPα/CD172a–


CD8α–

CD11c+

CD24–

CLEC9a–

CX3CR1+

EpCAM/TROP1–

F4/80+

Fcγ RI/CD64+


Integrin αM/CD11b+

Langerin/CD207– MHC class II+

SIRPα/CD172a+

XCR1–


EpCAM/TROP1–



Langerin/CD207– MHC class II+

SIRPα/CD172a–/low

Cell Surface MarkersCD11c+

CD24+

CLEC9a–

DC-SIGN/CD209+

DEC-205/CD205+

EpCAM/TROP1+

F4/80+


Integrin αM/CD11b+

Langerin/CD207+

MHC class II+

SIRPα/CD172a+

Langerhans CellUnique origin from a yolk sac-derived erythromyeloid progenitor cell or a fetal liver-derived monocyte rather than a bone marrow-derived preDC.

Integrin αE/CD103– CD11b– CD207– Dermal DC

Integrin αE/CD103– CD11b+ CD207– Dermal DC

Integrin αE/CD103– CD11b– CD207+ Dermal DC

CD8α+ Classical DCCell Surface MarkersCD4–

CD8α+

CD11c+

CD24+

CLEC9a+

DC-SIGN/CD209–

DEC-205/CD205+

F4/80–

IGSF4A/SynCAM1/Necl2+

Integrin αE/CD103+/–

Integrin αM/CD11b–

Langerin/CD207+/–

MHC class II+

SIRPα/CD172a–

XCR1+

Intracellular MarkersBatf3+

IRF8+

Secreted MoleculesIL-12

CD11b+ Classical DCCell Surface MarkersCD4+/–

CD8α–

CD11c+

CD24+

CLEC9a–

DCIR2+

DC-SIGN/CD209+

DEC-205/CD205+

F4/80+

IGSF4A/SynCAM1/Necl2–

Integrin αM/CD11b+

Langerin/CD207–

MHC class II+

SIRPα/CD172a+

XCR1–

Secreted MoleculesIFN-γIL-2IL-6

Plasmacytoid DCCell Surface MarkersB220/CD45 R+

Bst-2/PDCA-1+

CD11c+

CLEC9a+

DC-SIGN/CD209+


Ly6C+

MHC class II+

Siglec-H+

SIRPα/CD172+

Intracellular MarkersTLR7+

TLR9+

Secreted MoleculesIFN-αIFN-βIL-6TNF-α

Absent from steady state lymphoid organs and tissues; Inflammatory DCs differentiate from monocytes that are recruited to sites of inflammation.Cell Surface MarkersCD11c+

CD107b/Mac-3+

F4/80+

Fcγ RI/CD64+

Fcε RI+

Integrin αM/CD11b+

Ly6C+

MHC class II+

MMR/CD206+

Secreted MoleculesIL-12NOTNF-α

Human Inflammatory DCsin Peripheral Tissues & Draining

Lymph Nodes During Inflammation

Inflammatory DCAbsent from steady state lymphoid organs and tissues; Inflammatory DCs differentiate from monocytes that are recruited to sites of inflammation.Cell Surface MarkersLin–

CD1a+

CD1c/BDCA-1+

CD11c+

CD14+

Fcε RIα+

Fcγ RI/CD64+

HLA-DR+

Integrin αM/CD11b+/–

MMR/CD206+

SIRPα/CD172a+

Secreted MoleculesIL-23NOTNF-α

Integrin αE/CD103+ CD11b– Classical DCCell Surface MarkersCD4–

CD8α–

CD11c+

CD24+

CLEC9a+

CX3CR1–

DC-SIGN/CD209–

DEC-205/CD205+

EpCAM/TROP1–

F4/80–

Integrin αE/CD103+


Langerin/CD207+

MHC class II+

SIRPα/CD172a–

XCR1+

Integrin αE/CD103– CD11b+ Classical DC

Integrin αE/CD103+ CD11b+ Classical DC


CD8α–

CD11c+

CD24+/–

CLEC9a–

CX3CR1+

EpCAM/TROP1–

F4/80+

Fcγ RI/CD64+


Integrin αM/CD11b+

Langerin/CD207–

MHC class II+

SIRPα/CD172a+

XCR1–

Plasmacytoid DC

Integrin αE/CD103+ CD11b– Classical DC

Plasmacytoid DC

Integrin αE/CD103– CD11b+ Classical DC

Cell Surface MarkersB220/CD45 R+

Bst-2/PDCA-1+

CD11c+

CLEC9a+

DC-SIGN/CD209+


Ly6C+

MHC class II+

Siglec-H+

SIRPα/CD172a+


TLR9+



CD8α–

CD11c+

CD24+

CLEC9a–

CX3CR1–

DC-SIGN/CD209+

EpCAM/TROP1–

F4/80–

Integrin αE/CD103+

Integrin αM/CD11b+

Langerin/CD207–

MHC class II+

SIRPα/CD172a–

XCR1– Cell Surface MarkersCD4–

CD8α–

CD11c+

CD24+

CLEC9a+

CX3CR1–

DC-SIGN/CD209–

DEC-205/CD205+

EpCAM/TROP1–

F4/80–

Integrin αE/CD103+


Langerin/CD207+

MHC class II+

SIRPα/CD172a–

XCR1+

Cell Surface MarkersLin–

CD1a–

CD1c/BDCA-1+

CD11c+

CD141/BDCA-3+/–

HLA-DR+

Integrin αM/CD11blow

Secreted MoleculesIL-1βIL-6IL-10IL-12TNF-α

CD141/BDCA-3+ Classical DC (CD8α+-like DC)

CD1c/BDCA-1+ Classical DC (CD11b+-like DC)

Plasmacytoid DC


CD1a–

CD1c/BDCA-1–

CD11c+

CD141/BDCA-3+

CLEC9a+

DEC-205/CD205high

HLA-DR+



XCR1+


ID2+

IRF8+



CD1a–

CD11clow

DLEC/CLEC4C/BDCA-2+

HLA-DR+

IL-3 Rα/CD123+

Neuropilin-1/BDCA-4+


TLR9+

Secreted MoleculesIFN-αIFN-βIL-6TNF-αCell Surface Markers

CD4–

CD8α–

CD11c+

CD24+/–

CLEC9a–

CX3CR1+

EpCAM/TROP1–

F4/80+

Fcγ RI/CD64+


Integrin αM/CD11b+

Langerin/CD207–

MHC class II+

SIRPα/CD172a+

XCR1–

Cell Surface MarkersB220/CD45 R+

Bst-2/PDCA-1+

CD11c+

CLEC9a+

DC-SIGN/CD209+


Ly6C+

MHC class II+

Siglec-H+

SIRPα/CD172a+


TLR9+


Langerhans CellIf similar to mouse, human Langerhans cells originate from yolk sac-derived erythromyeloid progenitor cells or fetal liver-derived monocytes.Cell Surface MarkersLin–

CD1a+

CD1c/BDCA-1+

CD11c+

CD14–

E-Cadherin+

EpCAM/TROP1+

HLA-DR+

Integrin αM/CD11b+/–

Langerin/CD207+

SIRPα/CD172a+

CD141/BDCA-3+ Classical DC (CD8α+-like DC)Cell Surface MarkersLin–

CD1a–

CD1c/BDCA-1–

CD11c+

CD141/BDCA-3+

CLEC9a+

DEC-205/CD205high

HLA-DR+



XCR1+


ID2+

IRF8+


CD1c/BDCA-1+ Classical DC (CD11b+-like DC)Cell Surface MarkersLin–

CD1a–

CD1c/BDCA-1+

CD11c+

CD141/BDCA-3+/–

HLA-DR+


Secreted MoleculesIL-1βIL-6IL-10IL-12TNF-α

Plasmacytoid DCCell Surface MarkersLin–

CD1a–

CD11clow

DLEC/CLEC4C/BDCA-2+

HLA-DR+

IL-3 Rα/CD123+

Neuropilin-1/BDCA-4+


TLR9+



CD1a–

CD1c/BDCA-1+/–

CD11c+

CD14+

CD163–/+

DC-SIGN/CD209+

EpCAM/TROP1–

HLA-DR+

Langerin/CD207–

CD14+ Dermal DCs

CD1a+ CD1c/BDCA-1+ Dermal DCs

CD1a+ CD141/BDCA-3+ Dermal DCs


CD1a+

CD1c/BDCA-1+

CD11c+

CD14–

CD141/BDCA-3–/low

CD163–

DC-SIGN/CD209–

EpCAM/TROP1–

HLA-DR+

Integrin αM/CD11bhigh

Langerin/CD207–

SIRPα/CD172a+


CD1a+

CD1c/BDCA-1low/int

CD11clow

CD14–

CD141/BDCA-3+

CD163–

CLEC9a+

DC-SIGN/CD209–

EpCAM/TROP1–

HLA-DR+


Integrin αM/CD11bhigh

Langerin/CD207–

SIRPα/CD172a+

XCR1+

Dendritic Cells (DCs) provide a crucial link between the innate and adaptive immune response. This occurs primarily through their ability to capture, process, and present antigens to T cells, and regulate T cell functions in immunity and tolerance. This illustration highlights distinct DC subsets that exist in both human and mouse. The different DC subsets respond to distinct self and foreign antigen challenges, and exhibit different functional specialization. These subtypes can be characterized based on their tissue distribution, cell surface markers, and transcriptional programs.

RnDSy-lu-2945

Mouse and Human Dendritic Cell Subsets

NOTE: This poster conveys a general overview and should be considered neither comprehensive nor definitive. The details of this information are understood to be subject to interpretation. © 2017 R&D Systems

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Mouse and Human Dendritic Cell Subsets · F4/80 Integrin αE/CD103 ... Mouse and Human Dendritic...

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Transcript of Mouse and Human Dendritic Cell Subsets · F4/80 Integrin αE/CD103 ... Mouse and Human Dendritic...