Lab

This

Su

Fig

     

Fig

b on a Chip 

s journal is © Th

upplemen

gure S1. Hig

                 

gure S2. The

e Royal Society o

nt

gh-magnifica

e same herm

of Chemistry 201

ation image o

maphrodite in

12 

of the 10 μm

its chamber

m wide base a

at different t

and the pictur

time points.

re of cross se

J. Name.,

ection.

ARTI

2014, 00, 1‐3 

 

ICLE 

| 16 

Electronic Supplementary Material (ESI) for Lab on a Chip.This journal is © The Royal Society of Chemistry 2014

Lab

This

Fig

wh

b on a Chip 

s journal is © Th

gure S3. Th

hich have the

e Royal Society o

he three poin

e same size.

of Chemistry 201

nts (marked

12 

as red) weree used to deefine the lenggth and widt

J. Name.,

th of the 16

ARTI

2014, 00, 1‐3 

counting ar

ICLE 

| 17 

reas

Lab

This

Figpas

 

 

 

Ta

ch

ch

  

     

 

 

 

 

b on a Chip 

s journal is © Th

gure S4. Thessing through

ble S1. The t

hannel1 47.5 68.8   

hannel9  c          

                      

e Royal Society o

e original “sth different ch

time points o

channel2 17.7    

channel10 8.5 14.5 21.5  

of Chemistry 201

tandard deviahannels. The

of the L1 wor

channel3 3.9 8.9 14.7  

channel11 44.1    

12 

ation” signale number on

rms passing 

channel4

channel1225.543.7

ls from the Vtop of each p

through eac

channel55.4

channel1

Video S1. Pepeak represe

h channel in

5 channe

3 channel

aks of differents the chann

 the Video S

el6 chann7.19.4  

14 channe9.1   

J. Name.,

ent color menel number.

S1. (unit: seco

nel7  chan1 4    

el15  chan1  12

19

ARTI

2014, 00, 1‐3 

ean worms

 

onds) 

nnel8    

nnel162.89.4  

ICLE 

| 18 

Lab

This

Ta

     

 

 

b on a Chip 

s journal is © Th

able S2. The

     

e Royal Society o

e progeny nu

of Chemistry 201

umber every 4

12 

4 hours of thhe worms in FFigure 4a.

J. Name.,

ARTI

2014, 00, 1‐3 

 

ICLE 

| 19 

Lab

This

Figthe“*”Fothimeoth

    

b on a Chip 

s journal is © Th

gure S5. Alle bottom 15 w” means the r wild type w

is microfluidechanical streher 12 worm

e Royal Society o

the N2 and worms are daworm was ei

worms in thedic experimeness during tras, 5 bagged d

of Chemistry 201

daf-2 progenaf-2 mutantsither bagged

e microfluidicnt, two wormansferring anduring their r

12 

ny traces froms. The numbed, or dead, or c experiment

ms (#1 and #7nd loading. Oreproduction

m one microer at the end lost after the

t, the baggin7) died earlyOne worm (#n period, the

ofluidic experof each tracee last progen

ng rate is 3/13 (before Day

#14) slipped bagging rate

riment. The te represents tny peak and t3 = 23%. Fory 2), which wout of its cha

e is 5/12 = 41

J. Name.,

top 13 wormthe total progthus censoredr daf-2(e137

was most likeamber by acc1.7%.

ARTI

2014, 00, 1‐3 

ms are N2 andgeny, and thed at that poin0) worms in

ely due to thecident. For th

ICLE 

| 20 

d e nt.

e he