Supplementary Table 1. Changes in clinical and biochemical parameters after discontinuation

of medroxyprogesterone acetate

0 mon 3 mon 6 mon 9 mon Reference range

Weight (kg) 101.8 95.4 89 88

FPG (mg/dl) 107 72 105 86 75~115

Insulin (IU/L) 44.5 — 40.6 — 2.6 -24.9

HbA1c (%) 6.4 5.4 5.2 5.0 4.3~6.3

Estradiol (pg/mL) 6 129 31 64 27~433

LH (IU/L) 0.58 1.12 3.3 5.27 2.12~10.89

FSH (IU/L) 4.8 1.63 4.6 4.01 3.85~8.78

LH/FSH ratio 0.12 0.69 0.72 1.31 1:1-2.9:1

DHEAS (μg/dL) 52.6 20.2 92.3 129.2 35~430

Prolactin (ng/mL) 25.1 17.3 7.8 12.5 10-25

Testosterone (ng/mL) 0.01 0.04 0.26 0.68 0.1~0.75

SHBG (nmol/L) — 17.46 16.46 15.23 32.4 - 128.0

Free androgen index1 — 0.79 5.48 15.49 < 5.36

Cortisol (μg/dL) 0.44 0.69 8.84 8.11 6.7~22.6

ACTH (pg/mL) <1.00 6.49 4.88 21.62 5-60

Androstenedione (ng/mL) 0.13 0.54 1.32 3.36 0.5~4.7

1Free androgen index: 100×testosterone (nmol/L)/SHBG (nmol/L)

Biochemical hyperandrogenism in Korean women was defined as follows: total testosterone

>0.68 ng/mL or free androgen index >5.36. FPG indicates fasting plasma glucose; LH,

Luteinizing hormone; FSH, follicle-stimulating hormone; DHEAS, dehydroepiandrosterone

sulfate; SHBG, Sex hormone-binding globulin; ACTH, adrenocorticotropic hormone

Supplementary Table 2. Combined pituitary stimulation test

　 Insulin 　 TRH 　 LHRH

　 Glucose

(mg/dL)

ACTH

(pg/mL)

Cortisol

(μg /dL)

　 TSH

(mU/L)

Prolactin

(μg/L)

　 LH

(IU/L)

FSH

(IU/L)

Basal 91

40

292

187

116

4.91 0.92 3.39 25.09 0.63 3.25

30min 101.4 3.82 3.53 102.4 5.72 4.65

60min 32.65 4.23 16.66 82.97 5.48 10.06

90min 14.43 2.57 11.42 55.1 2.25 10.79

120min 10.74 2.06 6.88 37.4 1.48 21.42

A combined pituitary function stimulation test was performed with an intravenous injection

of regular insulin (0.15 U/kg), thyrotropin-releasing hormone (TRH; 400 μg), and luteinizing-

hormone-releasing hormone (LHRH; 100 μg). ACTH, adrenocorticotrophic hormone; TSH,

thyroid stimulating hormone; LH, luteinizing hormone; FSH, follicle stimulating hormone.