Leukocyte recruitment in inflammation Yalin Emre and Abcam ... · PDF file CD40L Human ELISA...

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Transcript of Leukocyte recruitment in inflammation Yalin Emre and Abcam ... · PDF file CD40L Human ELISA...

  • Basal membrane Laminin α5 forms barrier Gap of laminin α5

    Pericyte

    α6β1 MMP

    Elastase

    RAC1 RHO

    ROS eNOS

    RHO RHOIP3

    PIP2 RAP1

    RAP1

    RAP1

    RAPL RAP1

    RAP1

    EPAC

    cAMPPLC

    DAG

    DOCK2

    VAV1/2/3

    Endothelial cell

    Leukocyte

    Chemokine

    Cell-cell contact and actin remodelling

    Transcellular

    Paracellular Blood vessel lumen

    Rolling

    Activation

    Arrest Transmigration

    Chemokine receptorInflammation

    PSGL-1 ESL-1

    L-Selectin

    P-Selectin E-Selectin

    CD99 ICAM-1 JAM-A

    CD99 LFA-1 LFA-1

    JAM-A JAM-C ICAM-2

    PECAM-1 CD99

    ALCAM

    LFA-1 MAC-1 LFA-1

    PECAM-1 CD99

    ALCAM

    ICAM-1 VCAM-1

    LFA-1 MAC-1 VLA-4

    TalinRAPL

    Talin RAPL

    Ca2+

    Ca2+

    Ca2+

    VE-cadherin VE-cadherin

    TNFα IL-1

    Leukocyte recruitment in inflammation

    www.abcam.com/cardiovascular Copyright © 2011 Abcam, All Rights Reserved.

    by Beat Imhof, Yalin Emre and Abcam

    Leukocyte Recruitment Card06 A4:Layout 21/01/2011 14:49 Page 1

  • Leukocyte related antibodies from Abcam: Product Clonality Applications Host Species Datasheet

    Reactivity www.abcam.com/ab…

    Antibodies

    CD162 [3E2.25.5(PL1)] M Flow Cyt, IHC-Fr, IHC-P, WB Ms Hu 19793

    CD31 P ICC/IF, IHC-Fr, IHC-P, WB Rb Hu 28364

    CD40L P ELISA, IHC-Fr, IHC-P, WB Rb Hu, Rat 2391

    CD62E [1.2B6] M ELISA, Flow Cyt, IHC-Fr, IP, WB Ms Hu, Pig 6630

    CD62L [IVA94] M Flow Cyt, IP Ms Cow 3920

    CD62P [AK-6] M Flow Cyt, IHC-Fr, IHC-P, IP, WB Ms Hu, RMk 6632

    Connexin 43 / GJA1 P ICC, ICC/IF, IF, IHC-Fr, IHC-P, WB Rb Hu, Ms, Rat, Chk, Cow, Hm, Mk 11370

    eNOS P IHC-Fr, IHC-P, WB Rb Hu, Ms, Rat, Cow, Dog, Pig 5589

    Fibrinogen P ELISA, FuncS, ICC/IF, IHC-P Rb Hu, Ms, Rb 34269

    ICAM1 [MEM-111] M ELISA, Flow Cyt, IP, WB Ms Hu, Rat, Cow 2213

    MMP14 [EP1264Y] M Flow Cyt, ICC/IF, IHC-P, IP, WB Rb Hu, Ms, Rat 51074

    MMP2 P Flow Cyt, ICC/IF, IHC-P, IP, WB Rb Hu 52756

    MMP9 - Whole molecule P ELISA, IHC-FoFr, IHC-Fr, IHC-P, IP, WB Rb Hu, Ms, Dog 38898

    PPAR alpha P ELISA, ICC/IF, IHC-Fr, IHC-P, WB Rb Ms 8934

    VCAM1 [324] M ICC, IHC (Methanol fixed), IHC-Fr, WB Rat Ms, Rat 78712

    VE Cadherin [BV9] M Flow Cyt, ICC, ICC/IF, IHC-Fr, IHC-P, IP, Neut, P, WB Ms Hu, Ms 7047

    Elisa kits

    CD40L Human ELISA Kit - 1 x 96 Well Plate ELISA - Hu 99991

    MMP2 Human ELISA kit - 1 x 96 Well Plate ELISA - Hu 100606

    MMP2 Mouse ELISA Kit - 1 x 96 Well Plate ELISA - Ms 100730

    VCAM1 Human ELISA Kit - 1 x 96 Well Plate ELISA - Hu 100661

    VCAM1 Mouse ELISA Kit - 1 x 96 Well Plate ELISA - Ms 100750

    Proteins

    CD31 protein (Tagged) ELISA, Inhib, SDS-PAGE, WB - - 39438

    CD40 protein (Fc Chimera Active) FuncS, SDS-PAGE - - 83917

    CD40L protein (Active) FuncS, SDS-PAGE - - 50160

    CD62E protein (His tag) SDS-PAGE - - 52148

    CD62L protein SDS-PAGE - - 53383

    CD62L protein (Fc Chimera) SDS-PAGE - - 83824

    CD62P protein SDS-PAGE - - 83582

    CD62P protein (Fc Chimera Active) FuncS, SDS-PAGE - - 83583

    Fibrinogen protein (Active) FuncS, SDS-PAGE - - 92791

    ICAM1 protein (Human) SDS-PAGE - - 82125

    MMP14 protein (Active) SDS-PAGE - - 54060

    MMP2 protein (Human) ELISA, SDS-PAGE, WB - - 40964

    MMP9 protein (Human) FuncS, SDS-PAGE, WB - - 39308

    PPAR alpha protein (His tag) GSA, Other, SDS-PAGE - - 81927

    VCAM1 protein (His tag) WB - - 82993

    17 5- 11 /1 0- P W

    Leukocyte recruitment in inflammation Upon inflammation, leukocytes are captured from the circulation. Recognition of chemokines expressed on endothelial cells by specific chemokine receptors (G protein coupled receptors) leads to leukocyte activation and slow rolling on the endothelial wall. Downstream activation of PLC, RHO, and RAP1 GTPase signaling pathways leads to the recruitment and activation of integrins on the leukocyte surface. Binding of integrins to their ligands (e.g. LFA-1 to ICAM-1) activates RAC1, RHO, and calcium signaling pathways. The consequence is the remodeling of the actin network and a decrease of endothelial cell contacts, thus initiating and favoring leukocyte extravasation.

    Produced in collaboration with Yalin Emre, PhD (Post doctoral fellow) and Beat A. Imhof, PhD, Professor and Chair, Department of Pathology and Immunology, Medical Faculty, Geneva, Switzerland.

    For more information vist: www.abcam.com/cardiovascular

    Abbreviation Full name

    eNOS Endothelial nitric oxide synthase ICAM Intercellular adhesion molecule JAM Junctional adhesion molecule LFA-1 Lymphocyte function-associated antigen 1 (αLβ2-integrin) MAC-1 Macrophage antigen 1 (CD11b, αMβ2-integrin) PECAM1 Platelet/endothelial-cell adhesion molecule 1 PLC Phospholipase C RAC1 RAS-related C3 botulinum substrate RAPL Regulator for cell adhesion and polarization enriched in lymphoid tissues RAP1 RAS-related protein 1 RHO-A RAS homologue gene-family member A ROS Reactive oxygen species VCAM-1 Vascular cell-adhesion molecule 1 (CD106) VLA-4 Very late antigen 4 (α4β1-integrin)

    Product highlights

    Connexin 43/GJA1 (ab11370)

    Clonality: P

    Applications: ICC, ICC/IF, IF, IHC-Fr, IHC-P, WB

    Reactivity: Hu, Ms, Rat, Chk, Cow, Hm, Mk

    MMP9 (ab38898)

    Clonality: P

    Applications: ELISA, IHC-FoFr, IHC-Fr, IHC-P, IP, WB

    Reactivity: Hu, Ms, Dog

    Leukocyte Recruitment Card06 A4:Layout 21/01/2011 14:49 Page 2