HIF-2
3
N H HIF-2 DAPI parp-1 +/+ parp-1 -/- N H Figure S1 Fig S1. Nuclear HIF-2 accumulation by hypoxia is reduced in parp-1 -/- immortalized MEFs. Immunofluorescence staining of parp-1 +/+ and parp-1 -/- immortalized MEFs incubated in normoxia (N) or hypoxia (H, 16 hours at 1 % O 2 ). Representative images from several independent experiments are shown.
description
Figure S1. parp-1 +/+. parp-1 -/-. H. N. H. N. HIF-2 . DAPI. - PowerPoint PPT Presentation
Transcript of HIF-2
N H
HIF-2
DAPI
parp-1 +/+ parp-1 -/-
N H
Figure S1
Fig S1. Nuclear HIF-2 accumulation by hypoxia is reduced in parp-1 -/- immortalized MEFs.
Immunofluorescence staining of parp-1 +/+ and parp-1 -/- immortalized MEFs incubated in normoxia (N) or
hypoxia (H, 16 hours at 1 % O2). Representative images from several independent experiments are shown.
Figure S2
Control
PARP-1siRNA:
PARP-1
β-Actin
HIF-1α
β-Actin
HIF-2α
Control
siRNA:
H
HIF-2α
β-Actin
HIF-1α
Control
siRNA:
H
Figure S2. Validation of silencing efficacy: siRNA of PARP-1, HIF-2 or HIF-1 (Cells were transfected with the correspondig siRNAs and 48h later, the expression levels of the targeted protein was evaluated by WB. HIF expression was analyzed after 16 hours of exposure to hypoxia (1% O2).
parp-1+/+
parp-1-/-
Figure S3
0
5
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15Liver
*
**0
25
50
75Kidney
0
5
10
15Brain
Epo
mRN
A e
xpre
ssio
n(f
old
indu
ction
)
N H N H N H
Figure S3. EPO mRNA levels were determined by Q-PCR from liver, kidney and brain . The level of mRNA in each sample was expressed as fold of the value in control wild-type mice. Numeric value represents mean of hypoxic induction in each genotype at 8 hours, respectively. (*) and (**) indicate p< 0.05 and p<0.001, with respect to wild-type mice.
