GAPDH–Mouse DECAprobe Templatetools.thermofisher.com/content/sfs/manuals/sp_7330.pdf ·...
1
Ambion, Inc. • An Applied Biosystems Business • +1 800-888-8804 • www.ambion.com • e-mail: [email protected] Catalog #: AM7330 6/5/2008 Catalog #: AM7330 GAPDH–Mouse DECAprobe Template Store at –20°C. Do not store in a frost-free freezer. Catalog # (P/N): AM7330 Amount: 0.5 μg Concentration: 10 ng/μL Storage Conditions: Store at –20°C. This product is shipped at ambient temperature. This in no way affects its high-quality performance. Upon receipt, store at –20°C. Avoid multiple freeze-thaw cycles. Do not store in a frost-free freezer. USER INFORMATION Product Description: The GAPDH–Mouse DECAprobe Template is a 905 bp fragment of the mouse glyceraldehyde 3-phosphate dehydrogenase (GAPDH) gene. This fragment sequence can be found in Genbank Accession #M32599 (nucleotides 213 to 1117 [1]). Applications: Preparation of GAPDH Probe This DNA template is ready to use as a template for random-primed synthesis of radiolabeled or nonisotopic DNA probe. Random-prime-labeled probe synthesized from this template can be used for Northern and dot blots to detect the presence of GAPDH mRNA using a 50% formamide hybridization buffer (e.g., Ambion ULTRAhyb ® Ultrasensitive Hybridization Buffer, P/N AM8670) at 42°C. The size of the mouse GAPDH mRNA is 1.4 kb [1]. Reference: 1. Sabath DE, Broome HE, and Prystowsky MB (1990) Gene 91:185–191. QUALITY CONTROL Nonspecific Endonuclease Activity: A sample is incubated for 14–16 hr with supercoiled plasmid DNA and analyzed by agarose gel electrophoresis. Exonuclease Activity: A sample is incubated for 14–16 hr with labeled double-stranded DNA, followed by PAGE analysis. RNase Activity: A sample is incubated for 14–16 hr with labeled RNA, followed by PAGE analysis. Functional Testing: The DECAprobe Template is tested functionally in a DECAprime™ II reaction (P/N AM1455–AM1456). The GAPDH–Mouse DECAprobe template migrates as a single band of the expected size (905 bp) on a 1% agarose gel. The sequence of the GAPDH–Mouse fragment has been confirmed by dideoxynucleotide sequencing. OTHER INFORMATION Material Safety Data Sheets: Material Safety Data Sheets (MSDSs) can be printed or downloaded from product-specific links on our website at the following address: www.ambion.com/techlib/msds. Alternatively, e-mail your request to [email protected]. Specify the catalog or part number(s) of the product(s), and we will e-mail the associated MSDSs unless you specify a preference for fax delivery. For customers without access to the internet or fax, our technical service department can fulfill MSDS requests placed by telephone or postal mail. (Requests for postal delivery require 1–2 weeks for processing.) Warranty and Liability: For research use only. Not for use in diagnostic procedures. Applied Biosystems is committed to delivering superior product quality and performance, supported by industry-leading global service and technical support teams. Warranty information for the accompanying consumable product is available at www.ambion.com/info/warranty in "Limited Warranty for Consumables," which is subject to the exclusions, conditions, exceptions, and limitations set forth under the caption “EXCLUSIONS, CONDITIONS, EXCEPTIONS, AND LIMITATIONS” in the full warranty statement. Please contact Applied Biosystems if you have any questions about our warranties or would like information about post-warranty support. Information in this document is subject to change without notice. Applied Biosystems assumes no responsibility for any errors that may appear in this document. Applied Biosystems disclaims all warranties with respect to this document, expressed or implied, including but not limited to those of merchantability or fitness for a particular purpose. In no event shall Applied Biosystems be liable, whether in contract, tort, warranty, or under any statute or on any other basis for special, incidental, indirect, punitive, multiple or consequential damages in connection with or arising from this document, including but not limited to the use thereof. Trademarks, Patents, and Licensing: Applied Biosystems, AB (Design), Ambion, Strip-EZ and ULTRAhyb are registered trademarks, and DECAprime is a trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries. All other trademarks are the sole property of their respective owners. © 2008 Ambion, Inc. All rights reserved. 4386515A Page 1 of 1
Transcript of GAPDH–Mouse DECAprobe Templatetools.thermofisher.com/content/sfs/manuals/sp_7330.pdf ·...
Ambion, Inc. • An Applied Biosystems Business • +1 800-888-8804 • www.ambion.com • e-mail: [email protected]
Catalog #: AM7330 6/5/2008
Catalog #: AM7330
GAPDH–MouseDECAprobe TemplateStore at –20°C.Do not store in a frost-free freezer.
Catalog # (P/N): AM7330
Amount: 0.5 μg
Concentration: 10 ng/μL
Storage Conditions: Store at –20°C. This product is shipped at ambient temperature. This in no way affects its high-quality performance.Upon receipt, store at –20°C. Avoid multiple freeze-thaw cycles. Do not store in a frost-free freezer.
USER INFORMATION
Product Description: The GAPDH–Mouse DECAprobe Template is a 905 bp fragment of the mouse glyceraldehyde 3-phosphatedehydrogenase (GAPDH) gene. This fragment sequence can be found in Genbank Accession #M32599 (nucleotides213 to 1117 [1]).
Applications: Preparation of GAPDH ProbeThis DNA template is ready to use as a template for random-primed synthesis of radiolabeled or nonisotopic DNAprobe. Random-prime-labeled probe synthesized from this template can be used for Northern and dot blots to detectthe presence of GAPDH mRNA using a 50% formamide hybridization buffer (e.g., Ambion ULTRAhyb® UltrasensitiveHybridization Buffer, P/N AM8670) at 42°C. The size of the mouse GAPDH mRNA is 1.4 kb [1].
Reference: 1. Sabath DE, Broome HE, and Prystowsky MB (1990) Gene 91:185–191.
QUALITY CONTROL
Nonspecific EndonucleaseActivity:
A sample is incubated for 14–16 hr with supercoiled plasmid DNA and analyzed by agarose gel electrophoresis.
Exonuclease Activity: A sample is incubated for 14–16 hr with labeled double-stranded DNA, followed by PAGE analysis.
RNase Activity: A sample is incubated for 14–16 hr with labeled RNA, followed by PAGE analysis.
Functional Testing: The DECAprobe Template is tested functionally in a DECAprime™ II reaction (P/N AM1455–AM1456). TheGAPDH–Mouse DECAprobe template migrates as a single band of the expected size (905 bp) on a 1% agarose gel.The sequence of the GAPDH–Mouse fragment has been confirmed by dideoxynucleotide sequencing.
OTHER INFORMATION
Material Safety Data Sheets: Material Safety Data Sheets (MSDSs) can be printed or downloaded from product-specific links on our website at the followingaddress: www.ambion.com/techlib/msds. Alternatively, e-mail your request to [email protected] the catalog or part number(s) of the product(s), and we will e-mail the associated MSDSs unless you specify a preference forfax delivery. For customers without access to the internet or fax, our technical service department can fulfill MSDS requests placed bytelephone or postal mail. (Requests for postal delivery require 1–2 weeks for processing.)
Warranty and Liability: For research use only. Not for use in diagnostic procedures.
Applied Biosystems is committed to delivering superior product quality and performance, supported by industry-leading global serviceand technical support teams. Warranty information for the accompanying consumable product is available atwww.ambion.com/info/warranty in "Limited Warranty for Consumables," which is subject to the exclusions, conditions, exceptions,and limitations set forth under the caption “EXCLUSIONS, CONDITIONS, EXCEPTIONS, AND LIMITATIONS” in the full warrantystatement. Please contact Applied Biosystems if you have any questions about our warranties or would like information aboutpost-warranty support.
Information in this document is subject to change without notice. Applied Biosystems assumes no responsibility for any errors thatmay appear in this document.
Applied Biosystems disclaims all warranties with respect to this document, expressed or implied, including but not limited to those ofmerchantability or fitness for a particular purpose. In no event shall Applied Biosystems be liable, whether in contract, tort, warranty,or under any statute or on any other basis for special, incidental, indirect, punitive, multiple or consequential damages in connectionwith or arising from this document, including but not limited to the use thereof.
Trademarks, Patents, andLicensing:
Applied Biosystems, AB (Design), Ambion, Strip-EZ and ULTRAhyb are registered trademarks, and DECAprime is a trademark ofApplera Corporation or its subsidiaries in the US and/or certain other countries. All other trademarks are the sole property of theirrespective owners.
© 2008 Ambion, Inc. All rights reserved. 4386515A
Page 1 of 1
