Bone marrow-derived mesenchymal stem cells (BMSCs) …10.1038/s41598-017... · Figure 2 GAPDH...

Bone marrow-derived mesenchymal stem cells (BMSCs) repair acute necrotized

pancreatitis by secreting microRNA-9 to target the NF-B1/p50 gene in rats

Daohai Qian1,2,3, Ge Wei2, Chenglei Xu2, Zhigang He2, Jie Hua2, Jian Li2, Qili Hu2,

Shengping Lin4, Jian Gong2, Hongbo Meng2, Bo Zhou2, Hongfei Teng2, Zhenshun

Song2*

1, Department of General Surgery, Yijishan Hospital, Wannan Medical College, Wuhu,

Anhui 241001, China

2, Department of General Surgery, Shanghai Tenth Peoples Hospital, affiliated to

Tongji University School of Medicine, Shanghai 200072, China

3, Department of Pharmacology and Pharmaceutical Sciences, USC School of

Pharmacy, Los Angeles, California 90089, USA

4, Intensive Care Unit, Sir Run Run Shaw Hospital, affiliated to Zhejiang University of

Medicine, Hangzhou, Zhejiang 310058, China

Daohai Qian, Ge Wei and Chenglei Xu are equal to this work.

*Corresponding author: Zhenshun Song, M.D., Ph.D.

Department of General Surgery, Shanghai Tenth Peoples Hospital, Tongji University

School of Medicine, 301 Yanchang Road, Shanghai 200072, China.

Tel: +86 137 6120 5962,

Fax: 021-6630-7365,

E-mail: [email protected]

mailto:[email protected]

Figure 1

pri-miR-9-PCDH-CMV-MCS-EF1-GFP-T2A-Puro

BMSCs pri-miR-9 BMSCs

U6


Mature miR-9

marker marker


U6


marker

Mature miR-9 marker


U6

BMSCs

pri-miR-9 BMSCs

Mature miR-9

marker

marker

Pri-miR-9

Marker=D2000 Takara Pri-miR-9-1=386bp

Mature miR-9

U6

Mature miR-9

U6

Mature miR-9

U6

NC Sham SAP PBS BMSCs Pri-miR-9-BMSCs Empty-virus-BMSCs

TuD-BMSCs


TuD-BMSCs


TuD-BMSCs

Sham SAP PBS BMSCs Pri-miR-9-BMSCs Empty-virus-BMSCs

TuD-BMSCs NC


TuD-BMSCs


TuD-BMSCs

PCDH-CMV-MCS-EF1-GFP-T2A-Puro

Figure 2

GAPDH

IB

GAPDH

IL-6

GAPDH

TNF-

SOD1

SOD2

IL-10

SOD1

IL-6

IL-1

IL-10

IB

IB


NC TuD-BMSCs

Sham SAP PBS BMSCs Pri-miR-9-BMSCs

Empty-virus-BMSCs NC

TuD-BMSCs


NC TuD-BMSCs

SOD2

IB

IL-1

TNF-

SOD1

SOD2

IB

IL-6

IL-10

IB

IL-1

TNF-


NC TuD-BMSCs


NC TuD-BMSCs


NC TuD-BMSCs


NC TuD-BMSCs


NC TuD-BMSCs


NC TuD-BMSCs


NC TuD-BMSCs


NC TuD-BMSCs


Empty-virus-BMSCs NC TuD-BMSCs




Empty-virus-BMSCs

NC TuD-BMSCs























marker

marker

marker

marker

marker

marker

marker

marker

marker

Figure 2

p65

p50

IB

IB

GAPDH

p50

IB

IB

IB

p50

p65

IB

GAPDH

GAPDH

p65

Figure 3

Mature miR-9

U6

marker Sham

SAP+PBS miR-9a-5p agomir

miR-9a-5p control NC

marker Sham

SAP+PBS miR-9a-5p agomir


Sham SAP+PBS

miR-9a-5p agomir miR-9a-5p control

NC

Sham SAP+PBS


NC

Sham SAP+PBS


NC

Sham SAP+PBS miR-9a-5p agomir

miR-9a-5p control

NC

Mature miR-9

Mature miR-9

U6

U6

Figure 4

TNF-

SOD1

IL-6

IL-1

SOD2

IB

IB

IL-10

marker

marker

marker

marker

marker

marker

marker

GAPDH GAPDH

TNF-

SOD1

IL-6

IL-1

SOD2

IB

IB

IL-10

GAPDH

TNF-

SOD1

IL-6

IL-1

SOD2

IB

IB

IL-10

Sham SAP+PBS miR-9a-5p agomir miR-9a-5p control NC Sham SAP+PBS miR-9a-5p agomir miR-9a-5p control NC Sham SAP+PBS miR-9a-5p agomir miR-9a-5p control NC

Sham SAP+PBS miR-9a-5p agomir miR-9a-5p control NC





















Sham SAP+PBS miR-9a-5p agomir



Figure 4

p65

p50

IB

IB

GAPDH

CD68

p65 p50

IB

IB

GAPDH

CD68

p65

p50

IB

IB

GAPDH

CD68

Figure 5

PTF1

PDX1

Reg4

GAPDH Sham SAP PBS BMSCs Pri-miR-9-BMSCs


marker GAPDH

PTF1

PDX1

Reg4 Sham SAP PBS BMSCs Pri-miR-9-BMSCs



NC TuD-BMSCs


NC TuD-BMSCs

marker

marker

marker PTF1

PDX1

Reg4

GAPDH



Sham SAP PBS BMSCs Empty-virus-BMSCs

NC TuD-BMSCs

marker Pri-miR-9-BMSCs

Sham SAP PBS BMSCs Empty-virus-BMSCs

NC TuD-BMSCs

marker Pri-miR-9-BMSCs

Sham SAP PBS BMSCs

Empty-virus-BMSCs

NC TuD-BMSCs Pri-miR-9-BMSCs

Sham SAP PBS BMSCs

Empty-virus-BMSCs


Sham SAP PBS BMSCs

Empty-virus-BMSCs


Sham SAP PBS BMSCs

Empty-virus-BMSCs


PDX1

Reg4

PTF1

GAPDH

Figure 5

GAPDH

PTF1

PDX1

Reg4

Sham SAP+PBS miR-9a-5p agomir miR-9a-5p control NC Sham SAP+PBS miR-9a-5p agomir miR-9a-5p control NC Sham SAP+PBS miR-9a-5p agomir miR-9a-5p control NC

GAPDH

PTF1

PDX1

Reg4

GAPDH

PTF1

PDX1

Reg4 Sham SAP+PBS miR-9a-5p agomir miR-9a-5p control NC





Sham SAP+PBS miR-9a-5p agomir miR-9a-5p control NC Sham SAP+PBS miR-9a-5p agomir miR-9a-5p control NC



PTF1

PDX1

Reg4

GAPDH

Figure 6 Mature miR-9

U6

NC LPS

miR-9a-5p control

miR-9a-5p mimics

NC LPS

miR-9a-5p control

miR-9a-5p mimics

NC LPS

miR-9a-5p control

miR-9a-5p mimics

NC LPS

miR-9a-5p control

miR-9a-5p mimics

Mature miR-9

U6

Mature miR-9

U6

NC LPS

miR-9a-5p control

miR-9a-5p mimics

NC LPS

miR-9a-5p control

miR-9a-5p mimics

PBMC

p50

GAPDH

p50

GAPDH

p50

GAPDH

Figure 7

Mature miR-9

NC

Sham

Caerulein

1%NaT

3%NaT

NC

Sham

Caerulein

1%NaT

3%NaT

NC Sham

Caerulein 1%NaT

3%NaT

NC Sham

Caerulein

1%NaT 3%NaT NC

Sham Caerulein 1%NaT 3%NaT

NC Sham

Caerulein

1%NaT

3%NaT

NC Sham Caerulein 1%NaT 3%NaT

NC Sham Caerulein 1%NaT 3%NaT

NC Sham Caerulein 3%NaT




U6

Mature miR-9

U6

Mature miR-9

U6

Mature miR-9

U6

Mature miR-9

U6

Mature miR-9

U6

Supplementary picture. A lot of Cy3-miR-9 accumulated

in pancreatic lymph node after cy3-miR-9-BMSCs

transplantation.

Figure 8

Bone marrow-derived mesenchymal stem cells (BMSCs) …10.1038/s41598-017... · Figure 2 GAPDH...

Documents

Transcript of Bone marrow-derived mesenchymal stem cells (BMSCs) …10.1038/s41598-017... · Figure 2 GAPDH...

MOVIMENTO APOSTOLICO CATECHESIUn tempo il Primo e il Secondo Libro delle Cronache venivano chiamati “Il Primo e il Secondo Libro dei Paralipomeni” (Παραλειπόμενα),

Nature Chemical Biology: doi: 10.1038/nchembio Chemical Biology: doi: 10.1038/nchembio.367. Supplementary Fig. 1. EFOX produced by THP-1 cells coelute with those produced by RAW264.7

KADAR TNF-α, IL-10 DAN RASIO TNF-α/IL-10 SERUM PADA …

Corso Omega - Il Discepolato

Nature Neuroscience: doi:10.1038/nn · Nature Neuroscience: doi:10.1038/nn.4463. Supplementary Figure 2 AT1a-positive neurons in area postrema and PVN were not activated under the

IL-1β/IL-6/CRP and IL-18/ferritin: Distinct Inflammatory Programs in ...

Il Rettangolo Aureo

Published online:20 May 2002,DOI:10.1038/ni797 Immuno 508-10.pdf · 2021. 1. 2. · Published online:20 May 2002,DOI:10.1038/ni797 The antimicrobial peptide human α-defensin 5 (HD5)

Ένθεμα Δεξαμεθαζόνης (Ozurdex) «Στοχεύοντας νέα ......Il-7 Il-8 (CXCL8) Il-9 Il-10 Il-12 (p70) IL-13 Il-15 Il-17 Eotaxin (CCL11) b-FGF G-CSF GM-CSF

Il Liceo classico oggi

Nature, 2008, Doi:10.1038/nature07103 Semrah Kati 09.02.2009.

Sang-il Oum - KAIST

Chronic IL-1β-induced inflammation regulates epithelial-to ...10.1038/s41598-019-572… · Chronic IL-1β-induced inflammation regulates epithelial-to-mesen- chymal transition memory

Il tempo - TEDxUdine · 2018-11-08 · Simone Morandini, con il tema “Definire il tempo”, porta la parola della scienza, fondamentale per il nostro tempo in cerca di speg a-zioni

For Research Use Only GAPDH Polyclonal antibody · 10494-1-AP (GAPDH antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

IL-23 orchestrates mucosal responses to serotype ... · interferon (Ifn)-γ, interleukin (Il)-22 and Il-17 expression. Here we investigated the role of IL-23 in initiating these inflammatory

Il satellite terrestre

Nature , 2008, Doi:10.1038/nature07103

or IL-17–Producing

GAPDH Aggregates Promote Aβ Amyloidogenesis Glyceraldehyde ...