Beta Catenin MonoclonalantibodyCatalog Number:66379-1-Ig 24 Publications

For Research Use Only

www.ptglab.com

Basic Information Catalog Number:66379-1-Ig

Size:150ul , Concentration: 1000 μg/ml byBradford method using BSA as thestandard;

Source:Mouse

Isotype:IgG1

GenBank Accession Number:NM_001904

GeneID (NCBI):1499

Full Name:catenin (cadherin-associated protein),beta 1, 88kDa

Calculated MW:781 aa, 86 kDa

Observed MW:92 kDa

Purification Method:Protein A purification

CloneNo.:1B8A1

Recommended Dilutions:WB 1:5000-1:50000 IP 0.5-4.0 ug for IP and 1:1000-1:4000for WBIHC 1:2500-1:10000 IF 1:50-1:500

Applications Tested Applications:IF, IHC, IP, WB,ELISA

Cited Applications:CoIP, IF, IHC, WB

Species Specificity:human, mouse, rat, pig

Cited Species:human, mouse, pig, rat

Note-IHC: suggested antigen retrieval withTE buffer pH 9.0; (*) Alternatively, antigenretrieval may be performed with citratebuffer pH 6.0

Positive Controls:

WB : HeLa cells, pig brain tissue, MCF-7 cells, HEK-293cells, HepG2 cells, LNCaP cells, Jurkat cells, HSC-T6cells, NIH/3T3 cells

IP : mouse brain tissue,

IHC : human liver cancer tissue, human breast cancertissue, rat colon tissue, mouse colon tissue, humancolon tissue, human skin cancer tissue

IF : MCF-7 cells, human skin cancer tissue, human livercancer tissue

Background Information β-Catenin, also known as CTNNB1, is an evolutionarily conserved, multifunctional intracellular protein. β-Cateninwas originally identified in cell adherens junctions (AJs) where it functions to bridge the cytoplasmic domain ofcadherins to a-catenin and the actin cytoskeleton. Besides its essential role in the AJs, β-catenin is also a keydownstream component of the canonical Wnt pathway that plays diverse and critical roles in embryonicdevelopment and adult tissue homeostasis. The Wnt/β-catenin pathway is also involved in the activation of otherintracellular messengers such as calcium fluxes, JNK, and SRC kinases. Deregulation of β-catenin activity isassociated with multiple diseases including cancers. (PMID: 22617422; 18334222)

Notable Publications Author Pubmed ID Journal ApplicationPanpan Zhang 30272329 Int J Mol Med WB

Linjun Hong 28912304 Reproduction WB,CoIP,IHC

Ting-Ru Shao 31759044 Life Sci IF, WB

Storage Storage:Store at -20°C. Stable for one year after shipment.Storage Buffer:PBS with 0.02% sodium azide and 50% glycerol pH 7.3.

Aliquoting is unnecessary for -20ºC storage

T: 1 (888) 4PTGLAB (1-888-478-4522) (toll freein USA), or 1(312) 455-8498 (outside USA)

E: proteintech@ptglab.com W: ptglab.com

For technical support and original validation data for this product please contact: This product is exclusively available under ProteintechGroup brand and is not available to purchase from anyother manufacturer.

Selected Validation Data

Various lysates were subjected to SDS PAGEfollowed by western blot with 66379-1-Ig (beta-Catenin antibody) at dilution of 1:10000 incubatedat room temperature for 1.5 hours.

IP result of anti-beta-Catenin (IP:66379-1-Ig, 4ug;Detection:66379-1-Ig 1:2000) with mouse braintissue lysate 4400 ug.

Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using66379-1-Ig (beta-Catenin antibody) at dilution of1:5000 (under 10x lens). Heat mediated antigenretrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using66379-1-Ig (beta-Catenin antibody) at dilution of1:5000 (under 40x lens). Heat mediated antigenretrieval with Tris-EDTA buffer (pH 9.0).

Immunofluorescent analysis of (4% PFA) fixedMCF-7 cells using 66379-1-Ig (beta-Cateninantibody) at dilution of 1:200 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).