Figure 1 B D DOX ( M) C Cell Proliferation (% Con) MCF-7MCF-7/DOX DOX ( M) 0.03% 38.79% 0.15% 0.10% 0.20% 0.05% MCF-7MCF-7/DOX ** * A MDA-MB-231 Cell Proliferation (% Con) MCF-7 MCF-7/DOX T47D 0520 DOX (M) ** MCF-7MCF-7/DOX + DOX (1 M) + PARP (uncleaved) PARP (cleaved) Tubulin Figure 2 B MCF-7MCF-7/DOX Invasion (No. of cells/HPF) * A CD Lung tumors/ mouse (%) MCF-7MCF-7/DOX * P < uPA MMP-9 MMP-2 MCF-7MCF-7/DOX A B ControlNS NS398 ( M) Cell Proliferation (% Con) Invasion (No. of cells/% control) Figure 3 C Cox-2 Actin MDA-MB-231 MCF-7 MCF-7/DOX T47D Control NS398 (50 M) * uPA MMP-9 MMP-2 NS398 ( M) D E Migration (No. of cells/% control) * Cox-2 : siRNA SCR Figure 4 E Gefitinib ( M) EGF + + 2 Actin pEGFR Cox-2 EGFR C h EGF EGFR Actin Cox-2 SCR EGFR SCR EGFR : siRNA EGFR Tubulin Cox-2 B A D Gefitinib EGFR Tubulin MDA-MD-231 MCF-7/DOX Invasion (No. of cells/% control) Control MDA-MB-231 MCF-7 MCF-7/DOX T47D ` Gefitinib ( M) 2 Cell Proliferation (% Con) 0 2 * ControlLY Cell Proliferation (% Con) AB C D + EGF ControlU0126 U0126 ( M) 5 10 EGF Actin Cox-2 pERK1/2 Actin Cox-2 ERK 1/2 pERK 1/2 Akt pAkt LY ( M) 10 pAkt LY ( M) + + 10 Actin Cox-2 EGF Invasion (No. of cells/% control) * 10 Invasion (No. of cells/% control) 10 U0126 ( M) Cell Proliferation (% Con) ` 10 0 * Figure 5 E SCR Cox-2 : siRNA Actin Cox-2 F MMP-9 MMP-2 GAPDH uPA Cox-2SCR : siRNA U0126 Control LY U0126 Control LY MMP-9 MMP-2 GAPDH uPA RT-PCR WB Figure 6 AB DC SCREP1 EP3 : siRNA EP1 EP3 SCR : siRNA Invasion (No. of cells/% control) SCR EP1 siRNA * EP1 : siRNA SCR MMP-2 MMP-9 uPA -Actin MCF-7 MCF-7/DOX EP1 EP2 EP3 EP4 PGE 2 + EP1 EP2 EP3 EP4 -Actin SCR EP3 siRNA * EP1 EP3 + + + + + PGE 2 17-PT-PGE2 Sulprostone AH6809 GAPDH uPA MMP2 MMP9 E