Fig. S1 Representative MS spectra of the validated 14-3 …10.1007/s00726...584.5 585.0 585.5 586.0...
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Transcript of Fig. S1 Representative MS spectra of the validated 14-3 …10.1007/s00726...584.5 585.0 585.5 586.0...
Fig. S1 Representative MS spectra of the validated 14-3-3ε interactors formed in native HCC cells.
A. 14-3-3ε (bait protein). B. ACTN4. C. YWHAQ. D. PRPS1. E. MAP3K7 (TAK1).
A
IPI00000816: 14-3-3 epsilon (YWHAE). VAGMDVELTVEER Charge:2+
B
IPI00013808: Alpha-actinin-4 (ACTN4). TINEVENQILTR Charge: 2+
tsw17 #3214-3346 RT: 35.11-35.87 AV: 16 NL: 1.68E6T: FTMS + p ESI Full ms [400.00-2000.00]
724.2 724.4 724.6 724.8 725.0 725.2 725.4 725.6 725.8 726.0 726.2 726.4 726.6m/z
0
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40
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50
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60
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Re
lativ
e A
bu
nda
nce
724.36
724.86
725.36
725.86 726.67726.00 726.33724.53
724.39
724.34
726.43724.89724.83 725.54 725.84725.04725.39
725.34 725.71 726.16 726.76
tsw05 #5058-5905 RT: 55.30-60.86 AV: 137 NL: 3.70E5T: FTMS + p ESI Full ms [400.00-2000.00]
714.5 715.0 715.5 716.0 716.5 717.0 717.5 718.0 718.5 719.0m/z
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Rela
tive A
bundance
715.39
715.89
716.90
716.39
717.40
717.90
715.83 718.40716.34 716.84715.34 718.90717.35714.82 718.24716.08 717.65
715.42
718.55 719.14718.03
714.55717.45
716.43717.78
717.06
1.5Da
1.5Da
C
IPI00018146: 14-3-3 protein theta (YWHAQ). YLIANATNPESK Charge: 2+
D
IPI00219616: Ribose-phosphate pyrophosphokinase 1 (PRPS1). LNVDFALIHK Charge: 2+
tsw18 #765-938 RT: 18.93-20.51 AV: 49 NL: 7.76E4T: FTMS + p ESI Full ms [400.00-2000.00]
660.8 661.0 661.2 661.4 661.6 661.8 662.0 662.2 662.4 662.6 662.8 663.0 663.2 663.4m/z
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Rela
tive A
bund
anc
e
660.84
661.35
661.85
662.35662.85
661.55 663.36661.31 661.64
662.34
662.59662.17 662.88 663.23662.01 662.80660.86 661.01
661.82
661.45661.89
663.05
tsw17 #3793-3948 RT: 38.57-39.45 AV: 19 NL: 3.60E6T: FTMS + p ESI Full ms [400.00-2000.00]
584.5 585.0 585.5 586.0 586.5 587.0 587.5 588.0 588.5 589.0 589.5 590.0 590.5 591.0 591.5m/z
0
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Re
lativ
e A
bu
nda
nce
585.34
585.84
588.36
586.34
588.86
589.36586.85 590.37587.35585.28584.36 590.87585.48584.86 589.86587.85
585.88
591.37
590.14588.19
586.64 588.68
585.79
590.64587.15
586.28
589.13
3.0Da
1.5Da
E
IPI00218566: Isoform 1A of Mitogen-activated protein kinase kinase kinase 7 (MAP3K7, TAK1).
AFIVELR Charge: 2+
tsw07 #6864-6926 RT: 56.40-56.76 AV: 10 NL: 6.84E4T: FTMS + p ESI Full ms [400.00-2000.00]
424.0 424.2 424.4 424.6 424.8 425.0 425.2 425.4 425.6 425.8 426.0 426.2 426.4 426.6 426.8m/z
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Rela
tive A
bun
dance
424.26
425.76
424.76
426.27
425.26425.15 426.77424.49424.24 424.99 426.29425.42424.10 426.00 426.12425.60
425.70
1.5Da
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