EVALUATION OF in vitro ANTIOXIDANT POTENTIAL … (500 μg / mL) showed 58% inhibition potential of...
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23 rd Congress of the International Union for Biochemistry and Molecular Biology 44 th Annual Meeting of the Brazilian Society for Biochemistry and Molecular Biology Foz do Iguau, PR, Brazil, August 24 th to 28 th , 2015 Brazilian Society for Biochemistry and Molecular Biology (SBBq) EVALUATION OF in vitro ANTIOXIDANT POTENTIAL OF EXTRACTS OF Cassytha filiformis (Lauraceae) Mayara Nunes Vitor Anjos 1 ; Priscilla Andrade de Moura 1 ; Ingridd Ayslane Torres de Araœjo Ribeiro 1 ; Luiz Nascimento de Araœjo Neto 2 ; Alexandre Gomes da Silva 1,3 ; MÆrcia Vanusa da Silva 1 ; Maria Tereza dos Santos Correia 1 . 1. Departamento de Bioqumica, Centro de CiŒncias Biolgicas, Universidade Federal de Pernambuco, Recife - PE, Brazil; 2. Departamento de Antibiticos, Centro de CiŒncias Biolgicas, Universidade Federal de Pernambuco, Recife - PE, Brazil; 3. Instituto Agronmico do Semi-Ærido, Campina Grande - PB, Brazil. The family Lauraceae consists of 52 genus and approximately 250 species, it is primarily composed by trees and shrubs rich in secondary metabolites that have different biological and pharmacological activities. Several species of this family are found in the Atlantic Forest, biome characterized by high species diversity and high degree of endemism. Cassytha filiformis is a specie perennial, parasitic and herbaceous, that is used in medicinal purpose in several parts of the world, as antiplatelet agent, vasorelaxant, antitrypanosomal agent, among others. The aim of this study was evaluate the antioxidant activity, using two complementary methods, and quantify total phenolics compounds of the extracts of C. filiformis in the phases hexane, chloroform, ethyl acetate and methanol. All extracts were obtained by cold extraction and the antioxidant activity was measured using DPPH (2,2-diphenyl-2-picrylhydrazyl) free radical scavenging assay, the total antioxidant activity was evaluated by phosphomolybdic acid. In turn, the dosage of total phenolics compounds was realized by the Folin-ciocalteau reagent, and the reference standard used was gallic acid. According to the results, the methanolic extract (500 μg / mL) showed 58% inhibition potential of free radicals indicating good antioxidant activity as compared with gallic acid standard (500 μg / mL) with inhibition of 91%. Regarding the reduction of the phosphomolybdenum complex the methanolic extract also showed best activity with 13.6% The significant antioxidant capacity in both methods is explained by the high concentration of phenolic compounds found in these extracts (312,5 mg GAE / g). The results obtained in the antioxidant activity assays revealed the potential of the species C. filiformis as source of natural antioxidants which may be used as promising agents in scavenging free radicals and, consequently, in the treatment of related diseases.
Transcript of EVALUATION OF in vitro ANTIOXIDANT POTENTIAL … (500 μg / mL) showed 58% inhibition potential of...
23rd
Congress of the International Union for Biochemistry and Molecular Biology 44
th Annual Meeting of the Brazilian Society for Biochemistry and Molecular Biology
Foz do Iguaçu, PR, Brazil, August 24th to 28th, 2015
Brazilian Society for Biochemistry and
Molecular Biology (SBBq)
EVALUATION OF in vitro ANTIOXIDANT POTENTIAL OF EXTRACTS OF
Cassytha filiformis (Lauraceae)
Mayara Nunes Vitor Anjos1; Priscilla Andrade de Moura1; Ingridd Ayslane Torres de Araújo Ribeiro
1; Luiz Nascimento de Araújo Neto2; Alexandre Gomes da
Silva1,3; Márcia Vanusa da Silva1; Maria Tereza dos Santos Correia1.
1. Departamento de Bioquímica, Centro de Ciências Biológicas, Universidade Federal de Pernambuco, Recife - PE, Brazil; 2. Departamento de Antibióticos,
Centro de Ciências Biológicas, Universidade Federal de Pernambuco, Recife - PE, Brazil; 3. Instituto Agronômico do Semi-árido, Campina Grande - PB, Brazil.
The family Lauraceae consists of 52 genus and approximately 250 species, it is primarily composed by trees and shrubs rich in secondary metabolites that have different biological and pharmacological activities. Several species of this family are found in the Atlantic Forest, biome characterized by high species diversity and high degree of endemism. Cassytha filiformis is a specie perennial, parasitic and herbaceous, that is used in medicinal purpose in several parts of the world, as antiplatelet agent, vasorelaxant, antitrypanosomal agent, among others. The aim of this study was evaluate the antioxidant activity, using two complementary methods, and quantify total phenolics compounds of the extracts of C. filiformis in the phases hexane, chloroform, ethyl acetate and methanol. All extracts were obtained by cold extraction and the antioxidant activity was measured using DPPH (2,2-diphenyl-2-picrylhydrazyl) free radical scavenging assay, the total antioxidant activity was evaluated by phosphomolybdic acid. In turn, the dosage of total phenolics compounds was realized by the Folin-ciocalteau reagent, and the reference standard used was gallic acid. According to the results, the methanolic extract (500 μg / mL) showed 58% inhibition potential of free radicals indicating
good antioxidant activity as compared with gallic acid standard (500 μg / mL) with
inhibition of 91%. Regarding the reduction of the phosphomolybdenum complex the methanolic extract also showed best activity with 13.6% The significant antioxidant capacity in both methods is explained by the high concentration of phenolic compounds found in these extracts (312,5 mg GAE / g). The results obtained in the antioxidant activity assays revealed the potential of the species C.
filiformis as source of natural antioxidants which may be used as promising agents in scavenging free radicals and, consequently, in the treatment of related diseases.
