Copenhagen, 5 March 2015 Identification of the most immunogenic/protective VLP-HA construct in mice...
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Transcript of Copenhagen, 5 March 2015 Identification of the most immunogenic/protective VLP-HA construct in mice...
Copenhagen, 5 March 2015
Identification of the most immunogenic/protective VLP-HA construct in mice
Department of Immunology
Development of a universal influenza vaccine based on tandem core technology
Schematic of VLP-HA constructs
Modified from Steel et al, mBio 2010
ΔHA stalk (HA292-553):
GGGG
VLP-HA constructsΔHA stalk (HA292-553):
HA292-351 :
HA306-474 :
HA403-474 :
LAH (HA420-474):
HA292-402 :
HA292-474 :
HA306-402 :
HA306-351 :
HA351-474 :
Immune response to VLP-HAs
Immunization with VLP-Has protects mice in vivo
ΔHA stalk (HA292-553):
HA306-474 :
HA403-474 :
LAH (HA420-474):
IgG-specific antibody response of mice vaccinated with VLP-HAs
ΔHA stalk (HA292-553):
HA306-474 :
HA403-474 :
LAH (HA420-474):
HA420-474 (LAH) of different HA subtypes
Ongoing Work
• Immunization with new VLPs in mice
• In vivo challenge-protection assay with H3N2
• Micro-neutralization and ADCC assays