CD3-APC-Alexa Fluor 700 PN B10823 … · The CD3 antigen is a complex of 5 polypeptide chains: γ,...
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For Beckman Coulter’s worldwide office locations and phone numbers, please visit “Contact Us” at www.beckmancoulter.com CD3-APC-Alexa Fluor 700 PN B10823 Antibody Specificity: Human CD3 Clone: UCHT1 Isotype: IgG1 mouse The CD3 antigen is a complex of 5 polypeptide chains: γ, δ, ε, ζ and η associated with the T cell receptor (TCR) complex. The CD3 chains are clustered in a group of two invariant dimers, γ/ε and δ/ε associated with a variable dimer which consists of ζ homodimers, or ζ/η, or ζ/γ FcR heterodimers (γ FcR being the γ chain of the Fc receptors), or γ FcR homodimers. The CD3 antigen is expressed by mature T lymphocytes and by a subset of thymocytes. u Hannet, I., Erkeller-Yuksel, F., Lydyard, P., Deneys, V., DeBruyère, M., «Developmental and maturational changes in human blood lymphocyte subpopulations», 1992, Immunol. Today, 13, 215- 218. u Sprent, J., «T lymphocytes and the thymus», 1989, Fundamental Immunology, Chap 4, 2nd Ed., 69-93. u Thibault, G., Bardos, P., «Compared TCR and CD3 expression on and cells. Evidence for the association of two TCR heterodimers with three CD3 chains in the TCR/CD3 complex», 1995, J. Immunol.,154, 3814-3820. u Shores, E.W., Love, P.E., «TCR -chain in T cell development and selection», 1997, Cur. Opin. Immunol., 9, 380-389. u Ono, S., Ohno, H., Saito, T., «Rapid turnover of the CD3 chain independent of the TCR-CD3 complex in normal T cells», 1995, Immunity, 2, 639-644. u Julius, M., Maroun, C.R., Haughn, L., «Distinct roles for CD4 and CD8 as coreceptors in antigen receptor signalling», 1993, Immunol. Today, 14, 177-183. u van Agthoven, A., Terhorst, C., Reinherz, E.L., Schlossman, S.F., «Characterization of T cell surface glycoproteins T1 and T3 present on all human peripheral T lymphocytes and functional mature T lymphocytes», 1981, Eur. J. Immunol., 11, 18-21. u Tunnacliffe, A., Olsson, C., Traunecker, A., Krissansen, G.W., Karjalainen, K., De la Hera, A., «The majority of CD3 epitopes are conferred by the chain», 1989, Leucocyte Typing IV, White Cell Differentiation Antigens. W. Knapp, et al., Eds., Oxford University Press, 295-296. u Bernard, A., Brottier, P., Georget, E., Lepage, V., Boumsell, L., «Joint report of the first international workshop on human leucocyte differentiation antigens by the investigators of the participating laboratories», 1984, Leucocyte Typing I, Bernard, A. et al., Springer Verlag, 9-135. Antigen Characteristics Antibody Characteristics References Other Name: T3, CD3 complex Gene Location: 11q23 Gene Name: CD3 Molecular Weight: 25-28, 21, 20, 16 and 22 kDa The UCHT1 monoclonal antibody reacts with the ε chain of the CD3 complex. UCHT1 antibody has a mitogenic effect on T lymphocytes. It does not react with those T cells lacking the TCR. It has been assigned to the CD3 cluster of differentiation at the 1st International Workshop on Human Leucocyte Differentiation Antigens in Paris, France, in 1982, and it was used as a CD3 reference antibody during the the 6th International Workshop on Human Leucocyte Differentiation Antigens in Kobe, Japan, in 1996 (WS Code: 6T-CD3) Size Volume Line Status Part # Purified 0.2 mg - - ASR IM1304 Purified 100 tests 2 mL - ASR IM1280 Biotin 0.2 mg - - RUO IM1301 FITC 100 tests 2 mL IOTest CE A07746 PE 100 tests 2 mL IOTest CE A07747 ECD 100 tests 1 mL IOTest CE A07748 PC5 100 tests 1 mL IOTest CE A07749 Size Volume Line Status Part # PC5.5 50 tests 0,5 mL IOTest ASR A66327 PC7 100 tests 1 mL IOTest CE 737657 APC 100 tests 1 mL IOTest CE IM2467 APC-A700 (1) 50 tests 0,5 mL IOTest ASR B10823 APC-A750 (2) 50 tests 0,5 mL IOTest CE A94680 Pacific Blue* 50 tests 0,5 mL IOTest ASR A93687 Krome Orange 50 tests 0,5 mL IOTest ASR B00068 Reagent Line: IOTest Format: Liquid Size: 0.5 mL Status: ASR Example of Results DS-17339A B2012-13506 Outside the US version ASR: Analyte Specific Reagent. Analytical and performance characteristics are not established. CE: CE-marked product RUO: For Research Use Only. Not for use in diagnostic procedures. Beckman Coulter, the stylized logo and IOTest are trademarks of Beckman Coulter, Inc. and are registered in the USPTO. *Alexa Fluor and Pacific Blue are registered trademarks of Molecular Probes, Inc. Formats available (1) APC-Alexa Fluor* 700 (2) APC-Alexa Fluor* 750 Main Functions The CD3 complex associated with the TCR is involved in the recognition of peptides bound to the major histocompatibility complex class I and II during the immune response. Normal whole blood sample
Transcript of CD3-APC-Alexa Fluor 700 PN B10823 … · The CD3 antigen is a complex of 5 polypeptide chains: γ,...
For Beckman Coulter’s worldwide office locations and phone numbers, please visit “Contact Us” at www.beckmancoulter.com
CD3-APC-Alexa Fluor 700 PN B10823Antibody
Specificity: HumanCD3Clone: UCHT1Isotype: IgG1mouse
TheCD3antigenisacomplexof5polypeptidechains:γ, δ, ε, ζandηassociatedwiththeTcellreceptor(TCR)complex.TheCD3chainsareclusteredinagroupoftwoinvariantdimers,γ/εandδ/εassociatedwithavariabledimerwhichconsistsofζhomodimers,orζ/η,orζ/γ FcRheterodimers(γ FcRbeingtheγchainoftheFcreceptors),orγ FcRhomodimers.TheCD3antigenisexpressedbymatureTlymphocytesandbyasubsetofthymocytes.
uHannet,I.,Erkeller-Yuksel,F.,Lydyard,P.,Deneys,V.,DeBruyère,M., «Developmental andmaturational changes inhumanbloodlymphocyte subpopulations», 1992, Immunol. Today, 13, 215-218.
uSprent,J.,«Tlymphocytesandthethymus»,1989,FundamentalImmunology,Chap4,2ndEd.,69-93.
uThibault,G.,Bardos,P.,«ComparedTCRandCD3expressiononandcells.EvidencefortheassociationoftwoTCRheterodimerswith three CD3 chains in the TCR/CD3 complex», 1995, J.Immunol.,154,3814-3820.
uShores,E.W.,Love,P.E.,«TCR-chaininTcelldevelopmentandselection»,1997,Cur.Opin.Immunol.,9,380-389.
uOno, S., Ohno, H., Saito, T., «Rapid turnover of the CD3 chainindependentoftheTCR-CD3complexinnormalTcells»,1995,Immunity,2,639-644.
uJulius, M., Maroun, C.R., Haughn, L., «Distinct roles for CD4andCD8ascoreceptors inantigenreceptorsignalling»,1993,Immunol.Today,14,177-183.
uvanAgthoven,A.,Terhorst,C.,Reinherz,E.L.,Schlossman,S.F.,«Characterization of T cell surface glycoproteins T1 and T3presentonall humanperipheralT lymphocytesand functionalmatureTlymphocytes»,1981,Eur.J.Immunol.,11,18-21.
uTunnacliffe, A., Olsson, C., Traunecker, A., Krissansen, G.W.,Karjalainen,K.,DelaHera,A.,«ThemajorityofCD3epitopesareconferredbythechain»,1989,LeucocyteTypingIV,WhiteCellDifferentiationAntigens.W.Knapp,etal.,Eds.,OxfordUniversityPress,295-296.
uBernard, A., Brottier, P., Georget, E., Lepage, V., Boumsell, L.,«Joint report of the first international workshop on humanleucocyte differentiation antigens by the investigators of theparticipatinglaboratories»,1984,LeucocyteTypingI,Bernard,A.etal.,SpringerVerlag,9-135.
Antigen Characteristics
Antibody Characteristics
References
OtherName: T3,CD3complexGeneLocation: 11q23GeneName: CD3
MolecularWeight: 25-28,21,20, 16and22kDa
TheUCHT1monoclonalantibody reactswith theε chainof theCD3complex.UCHT1antibodyhasamitogeniceffectonTlymphocytes.ItdoesnotreactwiththoseTcellslackingtheTCR.IthasbeenassignedtotheCD3clusterofdifferentiationatthe1stInternationalWorkshoponHumanLeucocyteDifferentiationAntigens inParis,France, in1982,anditwasusedasaCD3referenceantibodyduring the the6th InternationalWorkshoponHumanLeucocyteDifferentiationAntigensinKobe,Japan,in1996(WSCode:6T-CD3)
Size Volume Line Status Part#Purified 0.2mg - - ASR IM1304Purified 100tests 2mL - ASR IM1280Biotin 0.2mg - - RUO IM1301FITC 100tests 2mL IOTest CE A07746PE 100tests 2mL IOTest CE A07747ECD 100tests 1mL IOTest CE A07748PC5 100tests 1mL IOTest CE A07749
Size Volume Line Status Part#PC5.5 50tests 0,5mL IOTest ASR A66327PC7 100tests 1mL IOTest CE 737657APC 100tests 1mL IOTest CE IM2467APC-A700(1) 50tests 0,5mL IOTest ASR B10823APC-A750(2) 50tests 0,5mL IOTest CE A94680PacificBlue* 50tests 0,5mL IOTest ASR A93687KromeOrange 50tests 0,5mL IOTest ASR B00068
Reagent
Line: IOTestFormat: LiquidSize: 0.5mLStatus: ASR Example of Results
DS-17339A B2012-13506
Outside the US version
ASR:AnalyteSpecificReagent.Analyticalandperformancecharacteristicsarenotestablished.CE:CE-markedproductRUO:ForResearchUseOnly.Notforuseindiagnosticprocedures.
BeckmanCoulter,thestylizedlogoandIOTestaretrademarksofBeckmanCoulter,Inc.andareregisteredintheUSPTO.
*Alexa Fluor and Pacific Blue are registered trademarks of Molecular Probes, Inc.
Formats available
(1)APC-AlexaFluor*700(2)APC-AlexaFluor*750
Main Functions
TheCD3complexassociatedwith theTCR is involved in the recognitionofpeptidesboundtothemajorhistocompatibilitycomplexclassIandIIduringtheimmuneresponse.
Normalwholebloodsample
