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CD3-APC-Alexa Fluor 700 PN B10823Antibody

Specificity: HumanCD3Clone: UCHT1Isotype: IgG1mouse

TheCD3antigenisacomplexof5polypeptidechains:γ, δ, ε, ζandηassociatedwiththeTcellreceptor(TCR)complex.TheCD3chainsareclusteredinagroupoftwoinvariantdimers,γ/εandδ/εassociatedwithavariabledimerwhichconsistsofζhomodimers,orζ/η,orζ/γ FcRheterodimers(γ FcRbeingtheγchainoftheFcreceptors),orγ FcRhomodimers.TheCD3antigenisexpressedbymatureTlymphocytesandbyasubsetofthymocytes.

uHannet,I.,Erkeller-Yuksel,F.,Lydyard,P.,Deneys,V.,DeBruyère,M., «Developmental andmaturational changes inhumanbloodlymphocyte subpopulations», 1992, Immunol. Today, 13, 215-218.

uSprent,J.,«Tlymphocytesandthethymus»,1989,FundamentalImmunology,Chap4,2ndEd.,69-93.

uThibault,G.,Bardos,P.,«ComparedTCRandCD3expressiononandcells.EvidencefortheassociationoftwoTCRheterodimerswith three CD3 chains in the TCR/CD3 complex», 1995, J.Immunol.,154,3814-3820.

uShores,E.W.,Love,P.E.,«TCR-chaininTcelldevelopmentandselection»,1997,Cur.Opin.Immunol.,9,380-389.

uOno, S., Ohno, H., Saito, T., «Rapid turnover of the CD3 chainindependentoftheTCR-CD3complexinnormalTcells»,1995,Immunity,2,639-644.

uJulius, M., Maroun, C.R., Haughn, L., «Distinct roles for CD4andCD8ascoreceptors inantigenreceptorsignalling»,1993,Immunol.Today,14,177-183.

uvanAgthoven,A.,Terhorst,C.,Reinherz,E.L.,Schlossman,S.F.,«Characterization of T cell surface glycoproteins T1 and T3presentonall humanperipheralT lymphocytesand functionalmatureTlymphocytes»,1981,Eur.J.Immunol.,11,18-21.

uTunnacliffe, A., Olsson, C., Traunecker, A., Krissansen, G.W.,Karjalainen,K.,DelaHera,A.,«ThemajorityofCD3epitopesareconferredbythechain»,1989,LeucocyteTypingIV,WhiteCellDifferentiationAntigens.W.Knapp,etal.,Eds.,OxfordUniversityPress,295-296.

uBernard, A., Brottier, P., Georget, E., Lepage, V., Boumsell, L.,«Joint report of the first international workshop on humanleucocyte differentiation antigens by the investigators of theparticipatinglaboratories»,1984,LeucocyteTypingI,Bernard,A.etal.,SpringerVerlag,9-135.

Antigen Characteristics

Antibody Characteristics

References

OtherName: T3,CD3complexGeneLocation: 11q23GeneName: CD3

MolecularWeight: 25-28,21,20, 16and22kDa

TheUCHT1monoclonalantibody reactswith theε chainof theCD3complex.UCHT1antibodyhasamitogeniceffectonTlymphocytes.ItdoesnotreactwiththoseTcellslackingtheTCR.IthasbeenassignedtotheCD3clusterofdifferentiationatthe1stInternationalWorkshoponHumanLeucocyteDifferentiationAntigens inParis,France, in1982,anditwasusedasaCD3referenceantibodyduring the the6th InternationalWorkshoponHumanLeucocyteDifferentiationAntigensinKobe,Japan,in1996(WSCode:6T-CD3)

Size Volume Line Status Part#Purified 0.2mg - - ASR IM1304Purified 100tests 2mL - ASR IM1280Biotin 0.2mg - - RUO IM1301FITC 100tests 2mL IOTest CE A07746PE 100tests 2mL IOTest CE A07747ECD 100tests 1mL IOTest CE A07748PC5 100tests 1mL IOTest CE A07749

Size Volume Line Status Part#PC5.5 50tests 0,5mL IOTest ASR A66327PC7 100tests 1mL IOTest CE 737657APC 100tests 1mL IOTest CE IM2467APC-A700(1) 50tests 0,5mL IOTest ASR B10823APC-A750(2) 50tests 0,5mL IOTest CE A94680PacificBlue* 50tests 0,5mL IOTest ASR A93687KromeOrange 50tests 0,5mL IOTest ASR B00068

Reagent

Line: IOTestFormat: LiquidSize: 0.5mLStatus: ASR Example of Results

DS-17339A B2012-13506

Outside the US version

ASR:AnalyteSpecificReagent.Analyticalandperformancecharacteristicsarenotestablished.CE:CE-markedproductRUO:ForResearchUseOnly.Notforuseindiagnosticprocedures.

BeckmanCoulter,thestylizedlogoandIOTestaretrademarksofBeckmanCoulter,Inc.andareregisteredintheUSPTO.

*Alexa Fluor and Pacific Blue are registered trademarks of Molecular Probes, Inc.

Formats available

(1)APC-AlexaFluor*700(2)APC-AlexaFluor*750

Main Functions

TheCD3complexassociatedwith theTCR is involved in the recognitionofpeptidesboundtothemajorhistocompatibilitycomplexclassIandIIduringtheimmuneresponse.

Normalwholebloodsample