Ascentis® Express 5 μm Columns with Fused-Core® Particles ... · PDF filetransfer...

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Outperforms Popular 3 μm and 5 μm Columns Ascentis ® Express 5 μm Columns with Fused-Core ® Particles Achieve More Efficiency with Less Backpressure Leverage Benefits on Any HPLC System Utilize Eight Different Phases Maximize Performance

Transcript of Ascentis® Express 5 μm Columns with Fused-Core® Particles ... · PDF filetransfer...

Page 1: Ascentis® Express 5 μm Columns with Fused-Core® Particles ... · PDF filetransfer methods to any site or CRO with no ... Ascentis Express 5 μm columns are excellent choices for

Outperforms Popular 3 μm and 5 μm Columns

Ascentis® Express 5 μm Columns with Fused-Core® Particles

Achieve More Efficiency with Less Backpressure

Leverage Benefits on Any HPLC System

Utilize Eight Different Phases

Maximize Performance

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sigma-aldrich.com/express5

Ascentis Express 5 μm Columns

kpressure Concerns

Ascentis Express 5 µm columns provide a new choice for improving the performance of traditional HPLC systems. Ascentis Express provides the benefits of high speed and high efficiencies without the concerns of smaller particle columns. Due to the high efficiencies at low backpressures, Ascentis Express 5 µm columns can benefit conventional HPLC users with no drawbacks.

Features at a Glance• Outperforms popular 3 μm and 5 μm columns

• High effi ciencies at low backpressures

• Same instrument – no change of methods or sample preparation

• A total of eight diff erent phases in analytical and semi-prep dimensions

• Rugged design

Ascentis® Express 5 µmAchieve Faster Separations with No Backpressure Concerns

With the addition of the 5 micron particle to the Ascentis Express product line, end users can now boost the performance of their legacy HPLC systems and effortlessly transfer methods to any site or CRO with no concerns of incompatibility. The Ascentis Express product line is scalable from UHPLC to legacy HPLC systems. Furthermore, these columns provide researchers interested in ultra-high resolution HPLC the ability to reach new levels of resolution through column coupling, due to their low backpressure.

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3Order: 800-325-3010 (U.S.) 814-359-3441 (Global)

Small Diffusion PathThe new Fused-Core® particle consists of a 3.3 μm solid core and a 0.6 μm porous shell. A major benefit of the Fused-Core particle is the small diffusion path (0.6 μm) compared to conventional fully porous particles. The short diffusion path reduces axial dispersion of solutes and minimizes peak broadening. In fact, Ascentis Express 5 μm columns are able to achieve greater speed and efficiency than any other 5 μm particle based column. This means that Ascentis Express 5 μm becomes the standard column for all of your 5 μm based methods.

Beyond the new standard column for all 5 μm based methods, Ascentis Express 5 μm columns are excellent choices for bioanalytical LC/MS methods. These columns excel under high flow rates and the high throughput demands of these methods. Furthermore, the large particle format provides an extremely rugged HPLC column.

Now available in a total of eight different phases: C18, F5, C8, Pheny-Hexyl, ES-Cyano, HILIC (Si), RP-Amide, and OH5Available in 17 dimensions from 2 cm x 2.1 mm I.D. to 25 cm x 4.6 mm I.D. and a total of eight phases makes the conversion of current methods seamless.

Key benefits of the eight phases include:

• Great combination of phases for method development

• Orthogonal selectivity

• High probability for separation

• F5 and ES-Cyano retentive both in RP and HILIC mobilephase conditions

Leverage Easy Implementation on Any System

0.5 μm

1.7 μm2.7 μm Solid Core

0.6 μm

3.3 μm5 μm Solid Core

Ascentis Express Fused-Core Particles

2.7 μm 5 μm

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Ascentis Express 5 μm Columns

Maximize Performance Efficiencies With Fast Fused-Core®

Fast HPLC with Shorter Diffusion Path

Fused-Core Particles Traditional Porous Particles

Consistent Bed Yields Sharper Peaks

Fused-Core Particles Traditional Porous Particles

Narrow Particle Size Distribution and Rugged Column Design

Fused-Core Particles Traditional Porous Particles

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5Order: 800-325-3010 (U.S.) 814-359-3441 (Global)

0 2 4 6 8 10Min

Ascentis® Express C18 5 μm, 15 cm x 4.6 mm ID 1.0 mL/min, N=23,422 for last peak, 80 Bar

Popular C18 5 μm, 15 cm x 4.6 mm ID 1.0 mL/min, N=13,633 for last peak, 72 Bar

Summary of Efficiency, Backpressure and SpeedColumn Efficiency (N) Toluene Backpressure (PSI) Runtime

Ascentis Express C18 5 μm, 15 cm x 4.6 mm 23,000 1,200 8 min

Popular C18 5 μm, 15 cm x 4.6 mm 14,000 1,100 16 min

Twice the Efficiency, Same Backpressure23,422

13,633

1,200 1,0800

5,000

10,000

15,000

20,000

25,000

Ascentis Express 5 μm Popular 5 μm

Efficiency (plates)

Backpressure (PSI)

3 μmTotally Porous

5 μmTotally Porous

5 μmFused-Core

0 100 200 300 400

360

Plates/Pressure in bar

Best Plates per Pressure

The 5 μm Ascentis Express Fused-Core particle has more than double the number of plates/pressure of the 5 μm totally porous particles and four times the number of plates/pressure of the 3 μm totally porous particles.

Outperform Popular 3 μm and 5 μm Columns

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Ascentis Express 5 μm Columns

Characteristics

Particle TypeShell

Thickness (µm)BET Surface Area (m2/g)

Average Pore Diameter (Å) Plates*

Pressure* (bar)

2.7 μm Ascentis Express Fused-Core 0.5 135 90 38,300 284

5 μm Ascentis Express Fused-Core 0.6 90 90 28,300 78

3 μm totally porous N/A 300 100 24,200 309

5 μm totally porous A N/A 300 100 14,600 100

5 μm totally porous B N/A 170 120 14,400 63

5 μm totally porous C N/A 450 100 15,300 120

* Plates and Pressure: reported for 1-chloro-4-nitrobenzene at the flow rate corresponding to the plate height minimum

Columns: 15 cm x 4.6 mmMobile Phase: 50:50 ACN:waterTemperature: 30 °C Injection: 1 μLInstrument: Agilent 1100 or Agilent 1200 with autosampler

10

100

1000

10000

1.00E+04 1.00E+05 1.00E+06

5 μm totally porous

3 μm totally porous

5 μm Fused-Core

t 0 (s

)

N (/)

Kinetic Plots of Various Particles Outperforms popular 3 μm and 5 μm columns under all conditions.

Compare Particle Attributes

Technical Conclusions• Plates/Pressure: 5 μm Fused-Core particle has more than double the plates/pressure of 5 μm totally porous particles.

– 4 times better than 3 μm particles

• 5 μm Fused-Core particles can provide faster, more efficient separations compared to 3 μm and 5 μm totally porous particles.

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7Order: 800-325-3010 (U.S.) 814-359-3441 (Global)

Get Started

Additional resources are available for helping you implement Ascentis Express HPLC Columns into your laboratory.

WebVisit sigma-aldrich.com/express for videos, product information, ordering and real time availability information.

EmailOur technical service staff is ready to answer questions.

EU: [email protected]: [email protected]

In PersonA technical seminar can be arranged on-site or via the web. Request via [email protected].

Ordering InformationAscentis Express 5 µm Fused-Core Columns

Ascentis Express 5 µm Guard Cartridges, Pk of 3

Description Cat. No.

Holder w/EXP Titanium Hybrid Ferrule (cartridge not included)

53500-U

Ascentis Express Universal Guard Holder

Guard Cartridge

Holder

For more information about Ascentis Express, visit sigma-aldrich.com/express5

I.D. Length Phases

10 mm 5 cm inquire

10 mm 10 cm inquire

10 mm 15 cm inquire

10 mm 25 cm inquire

Ascentis Express 5 µm Semi-Prep Columns

I.D. C18 F5 C8Pheny-Hexyl ES-Cyano HILIC (Si) OH5 RP-Amide

2.1 mm 50539-U 50633-U 50395-U 50496-U 50592-U 53520-U 53780-U 50776-U

3.0 mm 50541-U 50634-U 50396-U 50497-U 50593-U 53521-U 53781-U 50777-U

4.6 mm 50542-U 50635-U 50399-U 50498-U 50597-U 53523-U 53782-U 50779-U

I.D. (mm)

Length (cm)

2.1 2C18 F5 C8 Pheny-Hexyl ES-Cyano HILIC (Si) OH5 RP-Amide

50507-U 50603-U 50362-U 50442-U 50557-U 50255-U 50313-U 50732-U

2.1 3 50508-U 50604-U 50363-U 50443-U 50558-U 50256-U 50314-U 50733-U2.1 5 50509-U 50605-U 50364-U 50446-U 50559-U 50257-U 50317-U 50734-U2.1 7.5 50511-U 50607-U 50367-U 50451-U 50562-U 50258-U 50321-U 50735-U2.1 10 50517-U 50612-U 50368-U 50454-U 50563-U 50260-U 50322-U 50737-U2.1 15 50518-U 50613-U 50372-U 50455-U 50564-U 50261-U 50327-U 50739-U2.1 25 50521-U 50614-U 50373-U 50456-U 50566-U 50262-U 50328-U 50747-U3.0 3 50522-U 50615-U 50376-U 50459-U 50567-U 50264-U 50329-U 50749-U3.0 5 50523-U 50616-U 50377-U 50464-U 50568-U 50265-U 50335-U 50751-U3.0 7.5 50525-U 50619-U 50378-U 50466-U 50569-U 50268-U 50336-U 50752-U3.0 10 50526-U 50622-U 50381-U 50469-U 50570-U 50269-U 50338-U 50753-U3.0 15 50527-U 50623-U 50382-U 50470-U 50574-U 50270-U 50339-U 50758-U3.0 25 50528-U 50624-U 50385-U 50472-U 50575-U 50276-U 50341-U 50759-U4.6 3 50529-U 50625-U 50386-U 50474-U 50577-U 50278-U 50343-U 50767-U4.6 5 50530-U 50626-U 50389-U 50477-U 50581-U 50284-U 50344-U 50768-U4.6 7.5 50533-U 50627-U 50390-U 50479-U 50583-U 50286-U 50345-U 50769-U4.6 10 50536-U 50628-U 50391-U 50482-U 50585-U 50288-U 50346-U 50773-U4.6 15 50537-U 50631-U 50392-U 50483-U 50588-U 50289-U 50347-U 50774-U4.6 25 50538-U 50632-U 50394-U 50487-U 50591-U 50294-U 50348-U 50775-U

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