% of cells with >4N DNA content
4
0 5 10 15 20 25 30 % of cells with >4N DNA content MDA-MB-231 MDA-MB-468 0 20 50 100 250 nM, MK-0457, 48hr Online Resource 1
description
30. MDA-MB-468. MDA-MB-231. 25. 20. % of cells with >4N DNA content. 15. 10. 5. 0. 0. 20. 50. 100. 250. nM, MK-0457, 48hr. Online Resource 1. Online Resource 2. MDA-MB-468. Aurora B. Merge. Alpha Tubulin. Control. 20 nM MK-0457. 50 nM MK-0457. 100 nM MK-0457. 10 μ m. - PowerPoint PPT Presentation
Transcript of % of cells with >4N DNA content
0
5
10
15
20
25
30
% o
f ce
lls
wit
h >
4N D
NA
co
nte
nt
MDA-MB-231MDA-MB-468
0 20 50 100 250
nM, MK-0457, 48hr
Online Resource 1
MDA-MB-468
Control
20 nM MK-0457
50 nM MK-0457
100 nM MK-0457
Alpha Tubulin Aurora B Merge
Online Resource 2
10μm
BT-474
0 1.0 2.0 3.0 μM, vorinostat, 8hr IP:hsp90 (rat)
Aurora A
hsp90
Aurora B
Aurora A
hsp70
β-actin
IB: total cell lysates
BT-474 cells
0
10
20
30
40
50
60
70
Control
50 n
M M
K-045
7
250
nM M
K-045
7
1 μM
vorin
ostat
50 n
M M
K-045
7 + 1
μM
vorin
ostat
250
nM M
K-045
7 + 1
μM
vorin
ostat
% a
po
pto
sis
MK-0457 Vorinostat CI (nM) (nM) 30 300 0.824 50 500 0.682 100 1000 0.853 200 2000 0.566
0 0.2 0.4 0.6 0.8 1.00
0.2
0.4
0.6
0.8
1.0
Fractional Effect
CI
BT-474
a b
c
d
Online Resource 3
BT474
pSer10 H3
Aurora A
Survivin
β-actin
Aurora B
50
nM
MK
-04
57
Co
ntr
ol
20
nM
MK
-04
57
1 μ
M v
ori
no
sta
t
20 n
M M
K04
57+
vo
rin
ost
at
50 n
M M
K04
57+
vo
rin
ost
at
pAurora ApAurora B
Online Resource 4
MLN8237 vorinostat Fa CI(nM) (nM)125 1000 0.385 0.882150 1200 0.436 0.772175 1400 0.454 0.810200 1600 0.49 0.755225 1800 0.5 0.804250 2000 0.57 0.614
bc
Control
20 n
M M
LN8237
50 n
M M
LN8237
1 µM
Vorin
ostat
20 n
M M
LN8237
+ 1
µM
Vorin
ostat
50 n
M M
LN8237
+ 1
µM
Vorin
ostat
0102030405060
MDA-MB-231
% a
po
pto
sis
*
*
ap values 0.03 for 20 combo0.001 for 50 combo
0 0.2 0.4 0.6 0.8 1.00
0.5
1.0
1.5
2.0
Fractional Effect
CI
MDA-MB-468
