Μέθοδοι εξυγίανσης φυτικού πολλαπλασιαστικού υλικού

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Μέθοδοι εξυγίανσης φυτικού πολλαπλασιαστικού υλικού Θεοδώρα Καπαρή Κλάδος Φυτοπροστασίας ΙΓΕ 15 Οκτωβρίου, 2015 15/10/2015 Ινστιτούτο Γεωργικών Ερευνών 1
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Transcript of Μέθοδοι εξυγίανσης φυτικού πολλαπλασιαστικού υλικού

  • 15 , 2015

    15/10/2015 1

  • 1. 2. 3. 4. 5.

    15/10/2015 2

  • ,

    ,

    15/10/2015

    3

  • : RNA

    ( ) DNA

    ( )

    .

    RNA 4-20 . )

    :

    RNA (250-

    370 )

    :

    Io -

    15/10/2015 4

  • (, , .. )

    15/10/2015 5

  • 15/10/2015

    6

  • 1.

    2.

    3.

    4.

    5.

    6.

    .

    15/10/2015

    7

  • 19 .

    , , , , , ..

    . 15/10/2015 8

  • 30-40 oC 6 12 .

    .

    .

    .

    in vitro .

    15/10/2015 9

  • in vitro

    in vitro.

    in vitro .

    in vitro .

    15/10/2015 10

  • .

    4 oC 8 C

    .

    Potato spindle tuber viroid (PSTVd), 8 C, Cucumber palefruit

    viroid, 6 5 C, Apple scar skin viroid

    in vitro

    .

    15/10/2015 11

  • in vitro

    .

    1949, (virus titer)

    1952,

    .

    15/10/2015 12

  • .

    .

    :

    ) ,

    ) ,

    ) .

    15/10/2015 13

  • , :

    ) ,

    ) ,

    ) ,

    ) RNA

    .

    15/10/2015 14

  • Potato spindle tuber viroid (PSTVd) (Yijun Qi and Biao Ding)

    15/10/2015 15

  • in vitro (laminar flow).

    S (Murashige and Skoog, 1962), .

    , .

    15/10/2015 16

  • in vitro

    .

    A 1975 .

    , , , , , ..

    15/10/2015 17

  • / in vitro.

    - in vitro . 0.1-0.2 mm 15/10/2015

    18

  • .

    .

    .

    15/10/2015 19

  • T

    ( ) .

    (2-3 mm) . , (1-1.5 mm) 100%, .

    15/10/2015 20

  • 22-25 C 16

    15/10/2015 21

  • in vitro

    acyclquanosine, azidothymidine, ribavirin, acyclovir ..

    .

    15/10/2015 22

  • , .

    .

    (-196oC).

    in vitro .

    1997 Prunus (Plum pox virus).

    15/10/2015 23

  • :

    )

    )

    )

    )

    15/10/2015 24

    http://www.google.com.cy/url?sa=i&rct=j&q=&esrc=s&frm=1&source=images&cd=&cad=rja&uact=8&ved=0CAcQjRxqFQoTCMWKlOfk6ccCFYZWGgodLegKBQ&url=http%3A%2F%2Fwww.derecho-online.com%2F&psig=AFQjCNGXfMVQ59ur3nxDgxo95u3cABmAEQ&ust=1441882372068314https://www.google.com.cy/url?sa=i&rct=j&q=&esrc=s&frm=1&source=images&cd=&cad=rja&uact=8&ved=0CAUQjRxqFQoTCOqQsovl6ccCFQcyGgodMXgHRQ&url=https%3A%2F%2Fconservancy.umn.edu%2Fbitstream%2F90845%2F1%2FHSchneider_UROP2010.pdf&psig=AFQjCNGXfMVQ59ur3nxDgxo95u3cABmAEQ&ust=1441882372068314

  • 6 .

    Tatter leaf-citrange stunt virus .

    Prune dwarf virus (PDV), Prunus necrotic ring spot virus (PNRSV) Apple chlorotic leaf spot virus (ACLSV) , in vitro.

    PPV PNRSV in vitro.

    Citrus ringspot virus .

    ACLSV and PNRSV in vitro.

    15/10/2015 25

  • ELISA

    15/10/2015

    26

  • , ,

    , ,

    , ,

    , . , ,

    , ,

    , ..

    15/10/2015 27

  • -

    15/10/2015 28

  • Target crop Standard Year EPPO

    Bulletin

    Frui

    t /sm

    all f

    ruit

    cr

    ops

    Almond, apricot, peach and plum

    PM 4/30(1) 2001 31, 463478

    Cherry PM 4/29(1) 2001 31, 447462 Citrus trees and rootstocks PM 4/12(1) 1995 25, 737755

    Grapevine varieties and rootstocks

    PM 4/8(2) 1994 24, 347367

    Hazelnut PM 4/31(1) 2004 34, 145147

    Hop PM 4/16(2) 1997 27, 175184

    Malus, Pyrus and Cydonia spp.

    PM 4/27(1) 1999 & 2001

    29, 239252 & 31, 445446

    Olive trees and rootstocks PM 4/17(2) 2006 36, 7783 Ribes spp. PM 4/9(2) 2008 38, 1418

    Rubus spp. PM 4/10(2) 1994 24, 865873 Sambucus spp. PM 4/32(1) 2008 38, 1924

    Strawberry PM 4/11(2) 1994 24, 875889

    15/10/2015 29

  • 25 (VIONNER,

    , , ,

    , , ,

    , , ,

    , , AUTUMN ROYAL, EARLY

    SUPERIOR, BLACKROSE, BARLINCA,

    CRIMSON SEEDLESS, ITALIA, JULY MUSCAT,

    RED GLOBE, RUBY SEEDLESS, , VICTORIA, MONUKKA, )

    15/10/2015 30

  • 40 , 32 C 95% 16/8

    . 15/10/2015 31

  • in-vitro

    ,

    .

    15 ,

    23+2 C

    15/10/2015 32

  • , 1338-1 PINOT NOIR GR

    ITALIA

    1325-1 MATARO

    1329-1 CABERNET SAUVIGNON

    FANTASY

    1338-1 PINOT NOIR GR

    158/10 EARLY SUPERIOR

    1619-1 CARIGNAN

    1638-1 EMERALD RIESLING

    BLACK EMERALD

    1643-1 EARLY MUSCAT

    1613-1 SHIRAZ

    AUTUMN ROYAL

    1326-1 GEWURZTRAMINER

    1642-1 FLAME TOKAY

    1334-1 THOMPSON SEEDLESS

    1613-1 SHIRAZ

    1624-1 MUSCAT OTTONEL

    RED CLOBE

    1623-1 CABERNET FRANC

    1647-2 DELIGHT

    1625-1 CANNER

    1624-1 MUSCAT OTTONEL

    138-7 SUPLIMA

    CRIMSON SEEDLESS

    1615-1 PINOT NOIR

    1339-1 EMERALD SEEDLESS

    1639-1 BRONX SEEDLESS

    1327-1 CHENIN BLANC+

    1617-1 MERLOT

    PRIMA 1645-3 CHRISTMAS

    1616-1 MULLER THURGAN

    1337-1 MALVASIA BIANCA

    1617-1 MERLOT

    1641-1 SENECA

    PINOT BLANC 1328-1 CHARDONNAY

    1622-1 EMPEROR

    1341-1 CALMERIA

    1641-1 SENECA

    1621-1 QUEEN

    FANTASY SEEDLESS

    1333-1 GOLD

    1618-1 GRENACHE

    1620-1 ALLICANTE BOUCHET

    1621-1 QUEEN

    1332-1 CARDINAL

    AGIORGHTIKON 1335-1 CAMAY

    1646-2 MUSCAT FLAME

    1636-2 WHITE RIESLING

    1332-1 CARDINAL

    1340-1 FLAME SEEDLESS

    ORA

    1614-1 SEMILLON

    1637-1 SAINT EMILION

    1612-1 SYLVANER

    1340-1 FLAME SEEDLESS

    15/10/2015 33

  • , SL-98-15

    SL-98-15

    1627-1 COUDERC 1616

    SL-89-12-2

    SL-88-1-10

    SL-98-14

    SL-98-14

    SL-98-1

    1629-1 DOGRIDGE

    SL-87-6-9

    SL-89-15-2

    SL-98-13

    SL-98-13

    1331-1 5BB

    1632-1 99R

    SL-87-2-8 -

    SL-88-6-8

    SL-98-12

    SL-98-12

    1634-2 SALT CREEK

    SL-89-12-2

    SL-87-4-4

    SL-89-12-1

    SL-98-11

    SL-98-11

    1630-1 33EM

    SL-89-15-2

    SL-87-6-3

    SL-87-3-4

    SL-98-8

    SL-98-8

    1628-1 COUDERC 3309

    SL-87-5-4

    SL-88-8-2

    SL-89-9-5

    SL-98-6

    1336-1 OPPENHEIM

    SL-88-6-8

    SL-87-5-5

    SL-88-9-5

    SL-98-5

    SL-98-6

    1335-1 420A

    SL-89-7-1

    SL-88-9-3

    SL-88-7-1

    SL-98-3

    SL-98-5

    1633-1 110R

    SL-87-2-7

    SL-90-1-19

    SL-89-2-6

    SL-98-1

    SL-98-3

    1631-2 41B

    SL-88-6-9

    SL-90-1-15

    SL-87-3-6

    I

    25/10/2015 34

  • ,

    15/10/2015 35

  • : PLRV, PVA, PVS, PVX, PVY, PVM.

    : , 36 oC 90%, 40 .

    in vitro: , 0.5 mm

    in vitro:

    25/10/2015 36

  • 25/10/2015 37

  • (Royal Gold, Spring Crest, Red June, Arm King, Suellen Crepel, Fair Lane, Florida King, Flavour Top, Royal April, H. Harley, Bullards, , ),

    (Griotte DV Nord, Utah Giar, B. Lapine, B. Revershon),

    (Santa Rosa, Stanley, President, Red Beauty, Black Amber, Tende)

    (Palen, Dixie red, Brecaucia Licia, , Monique). 15/10/2015 38

  • in vitro

    in vitro

    :

    1) Lloyd and McCown medium for woody plants

    2) MS (Murashige and Skoog), 0.5mg 6-benzylaminopurine, 0.5 indole-3-butyric acid, 0.1 mg gibberellic acid,

    3) MS 100 mg inositol, 100 mg Adenine Sulfate.2H2O, 1 mg N6Benzyladenine, 0.4 mg Thiamine.HCl

    4) MS 100 mg inositol, 100 mg Adenine Sulfate.2H2O, 1 mg N6Benzyladenine, 0.4 mg Thiamine.HCl, 0.5 mg indole-3-butyric acid, 1 mg Pyridoxine, I mg Nicotinic acid

    15/10/2015 39

  • - ,

    in vitro - .

    15/10/2015 40

  • ,

    .

    7

    Spring Time 15 Red Haven 6

    Flavour Top 14 5

    13

    Stanley 23 12 Arm King 3

    President 22 May Grande 10 Red June 2

    Sanda Rosa 21 8 Spring Grest 1

    33 Royal gold 16 8

    B. Lapine 30 Spring Time 15 7

    28 Flavour Top 14 Red Haven 6

    Tente 26 . 13 5

    15/10/2015 41

  • 2 . 2 BLACK 2

    TARTARIAN

    1 . 2 1

    2 2 2

    . 2 RIVER CHOCK 1 1

    I 2 2 A 1 FLORIDA KING 1

    GREE GAGE 2 1 2 2 H. 1 1

    2 2 (RED BEAUTY) MONIQUE 1 BLACK TARTARIAN

    2 1 2

    1 . 1 1

    GREE GAGE 1 2

    2

    2 1

    2 PRESIDENT 1 Y 1

    2 1 . 2

    2 RIVER CHOCK 2

    2 1

    2 RED BEAUTY 1 1

    15/10/2015 42

  • 15/10/2015 43

  • , ,

    ,

    ,

    ,

    , ,

    15/10/2015 44

  • , , Salustiana, Page, SRA, Navel late, Star Ruby, Novela Anthous, , Intertonato, Berna , , , , , Shambar, , , Olinda, Marisol, Moro,

    Lane Late, Nova, Xo, Valencia

    15/10/2015 45

  • : Moro Lane Late Nova Page Star Ruby

    25/10/2015 46

  • E in vitro

    20 30C, 20 35C, 25 38C 5 40C. 22C

    38C 40C 56 .

    in vitro / in vitro

    25/10/2015 47

  • E

    in vitro 2-4 cm 1-3

    )Ribavirin (C81245) 20, 30, 40 50 mg/lt

    ) ethotrexate 30, 40 and 50 mg/lt 22C 30-50 ,

    in vitro / in vitro

    15/10/2015 48

  • E in vitro

    0

    20

    40

    60

    80

    100

    120

    1 2 3 4 5 6

    Ribavirin mg/lt

    Shoot survival %

    CTV elimination%

    15/10/2015 49

  • E ,

    in vitro

    in vitro CEVd HSVd.

    10 C 4

    4 C 4

    Ribavirin 40 50 mg/lt

    15/10/2015 50

  • E CEVd HSVd

    in vitro

    0

    5

    10

    15

    20

    25

    30

    22C/8 weeks 10C/5 weeks 4C/5 weeks

    Infected shoots number

    survival shoots number

    viroids elimination

    15/10/2015 51

  • 15/10/2015 52

  • ,

    Frost Valencia, Valencia Delta, Midnight, Champman

    Washington navel, Navelina, Nawhall, Gillette

    Lanelate, Fisher, Cara-cara ()

    Sanquinelli, Ruby, Tarocco, Moro

    Marsh seedless, Star Ruby, Rio Red, Oroblanco, Melogold,

    Chandler, Flame

    Clementine, Nova, Fortuna, Robinson, Murcott

    Sunburst, Kinnow, Ortanique, Ellendale,

    Orlando, Page, Monreal, Caffin, Sue-Linda Temple, Fina

    STG 77, STG 174, STG 201, STG 212, STG 2627

    52

    30, 38, 41, 48, 67

    15/10/2015

    53

  • :

    : ,

    , , , ,

    , , ,

    , , , ,

    ..

    : , ..

    15/10/2015 54

  • A , 2015 55